Exon grafting yields a “two active-site” lysozyme

Protein Design through Systematic Catalytic Loop Exchange in the (β/α)8 Fold

“…[16][17][18] Furthermore, recent work demonstrated that more profound changes in the enzymatic function are possible through loop swapping as a component of the directed evolution strategy. [19][20][21] On the other hand, the design of new enzymes by generating variability through point mutation of an existing gene has shown modest progress. 22 As stated above, for the natural evolution of proteins, this is likely because more drastic changes are needed.…”