Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors

Mielnichuk, Natalia; Joya, Constanza M.; Monachesi, María A.; Bertani, Romina P.

doi:10.1007/978-1-0716-3617-6_5

Methods in Molecular Biology

2024

DOI: 10.1007/978-1-0716-3617-6_5

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Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors

Natalia Mielnichuk,

Constanza M. Joya,

María A. Monachesi

et al.

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2024

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Silversol^®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways

Thakkar,

Gajera,

Godse

et al. 2024

Preprint

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Antibacterial effect of a colloidal nanosilver formulation Silversol was investigated against an antibioticresistant strain of Staphylococcus aureus. Lower concentrations of the test formulation exerted bacteriostatic and, its higher concentrations exerted bactericidal effect against this pathogen. Silversol at sub-lethal concentration was found to disturb multiple physiological traits of S. aureus such as growth, antibiotic susceptibility, membrane permeability, efflux, protein synthesis and export, biofilm and exopolysaccharide production, etc. Transcriptome data revealed the genes coding for transcriptional regulators, efflux machinery, transferases, beta-lactam resistance, oxidoreductases, metal homeostasis, virulence factors, and arginine biosynthesis to get expressed differently under influence of the test formulation. Genes (argG and argH) involved in arginine biosynthesis emerged among the major targets of Silversol in S. aureus.

show abstract

Silversol^®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways

Thakkar,

Gajera,

Godse

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors

Cited by 1 publication

References 23 publications

Silversol^®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways

Silversol^®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways

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Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors

Cited by 1 publication

References 23 publications

Silversol®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways

Silversol®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways

Contact Info

Product

Resources

About

Silversol^®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways

Silversol^®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways