EXOSC10/Rrp6 is essential for the eight-cell embryo/morula transition

Petit, Fabrice G.; Jamin, Soazik P.; Kernanec, Pierre-Yves; Becker, E.; Halet, Guillaume; Primig, Michael

doi:10.1016/j.ydbio.2021.12.010

Cited by 8 publications

(12 citation statements)

References 63 publications

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“…EXOSC10 is important for the 3' maturation of the 5.8s rRNA in yeast [7] and in pre-rRNA precursors processing in human [8]. Recent work using a mouse Exosc10 gene deletion and targeted inactivation via the Cre-lox system revealed an important role in spermatogonia [9], and a developmental arrest at the eight-cell/morula transition; we note that EXOSC10 was found to accumulate at the periphery of nucleolus precursor bodies (NPBs), which suggests an important role in embryonic rRNA processing and protein synthesis [10].…”

Section: Ivyspringmentioning

confidence: 61%

“…To specifically inactivate Exosc10 in the ovarian germ line, we used the STOCK Tg(Gdf9-iCre)5092Coo/J (Gdf9-iCre, The Jackson Laboratory, SN011062) mouse line [12], and the Exosc10 +/and Exosc10 f/f mouse lines [9,10]. Gdf9-iCre mice were crossed with Exosc10 +/mice to generate Exosc10 +/-; Gdf9-iCre mice.…”

Section: Micementioning

confidence: 99%

“…Mouse genotyping was performed at weaning and after euthanasia. Genomic DNA was extracted from tail tips and subjected to a standard PCR assay as described in Petit et al [10]. The set of primers used to discriminate the different Exosc10 alleles (wild type or +, knock-out or -, floxed or f, and conditional knock-out or cKO) and to detect the Gdf9-iCre transgene are listed in Supplementary Fig.…”

Section: Micementioning

confidence: 99%

“…To investigate the role of Exosc10 during oogenesis, we used a Cre/loxP system to generate a conditional mutant mouse line that lacks functional Exosc10 in oocytes. We initially crossed the mice harboring the Exosc10-null allele (previously described [9,10] and Fig. S1A) with the Gdf9-iCre mouse line to express the Cre recombinase in oocytes from the primordial follicle stage onwards [12].…”

Section: Targeted Inactivation Of Exosc10 In Oocytes Impairs the Estr...mentioning

confidence: 99%

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Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice

Demini¹,

Kervarrec²,

Guillot³

et al. 2023

Int. J. Biol. Sci.

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EXOSC10 is a catalytic subunit of the nuclear RNA exosome, and possesses a 3'-5' exoribonuclease activity. The enzyme processes and degrades different classes of RNAs. To delineate the role of EXOSC10 during oocyte growth, specific Exosc10 inactivation was performed in oocytes from the primordial follicle stage onward using the Gdf9-iCre; Exosc10 f/-mouse model (Exosc10 cKO(Gdf9) ). Exosc10 cKO(Gdf9) female mice are infertile. The onset of puberty and the estrus cycle in mutants are initially normal and ovaries contain all follicle classes. By the age of eight weeks, vaginal smears reveal irregular estrus cycles and mutant ovaries are completely depleted of follicles. Mutant oocytes retrieved from the oviduct are degenerated, and occasionally show an enlarged polar body, which may reflect a defective first meiotic division. Under fertilization conditions, the mutant oocytes do not enter into an embryonic development process. Furthermore, we conducted a comparative proteome analysis of wild type and Exosc10 knockout mouse ovaries, and identified EXOSC10-dependent proteins involved in many biological processes, such as meiotic cell cycle progression and oocyte maturation. Our results unambiguously demonstrate an essential role for EXOSC10 in oogenesis and may serve as a model for primary ovarian insufficiency in humans. Data are available via ProteomeXchange with identifier PXD039417.

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Section: Ivyspringmentioning

confidence: 61%

Section: Micementioning

confidence: 99%

Section: Micementioning

confidence: 99%

Section: Targeted Inactivation Of Exosc10 In Oocytes Impairs the Estr...mentioning

confidence: 99%

See 2 more Smart Citations

Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice

Demini¹,

Kervarrec²,

Guillot³

et al. 2023

Int. J. Biol. Sci.

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“…Unlike DIS3L2, however, we showed that DIS3 is an essential gene in mice, as evidenced by the analysis of both knock-out and point catalytic mutations of the catalytic domain (unpublished). Interestingly, this is also the case for the second nuclear exosome-bound exoribonuclease, RRP6, which was recently identified to be essential for mouse embryo development past the morula stage (17). Notably, essentially nothing is known about the role of DIS3L at the organismal level.…”

Section: Introductionmentioning

confidence: 86%

DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice

Brouze

Szpila

Czerwińska

et al. 2022

Preprint

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Among numerous enzymes involved in RNA decay, processive exoribonucleases are the most prominent group responsible for the degradation of the entire RNA molecules. The role of mammalian cytoplasmic 3′-5′ exonuclease DIS3L at the organismal level remained unknown. Herein we established knock-in and knock-out mouse models to study DIS3L functions in mice. DIS3L is indeed a subunit of the cytoplasmic exosome complex, which disruption leads to severe embryo degeneration and death in mice soon after implantation. These changes could not be prevented by supplementing extraembryonic tissue with functional DIS3L through the construction of chimeric embryos. Preimplantation Dis3l-/- embryos were unaffected in their morphology and ability to produce functional embryonic stem cells showing that DIS3L is not essential for cell viability. There were also no major changes in the transcriptome level for both embryonic stem cells and blastocysts, as revealed by RNA sequencing experiments. Notably, however, DIS3L knock-out led to inhibition of the global protein synthesis. These results point to the essential role of DIS3L in mRNA quality control pathways crucial for proper protein synthesis during embryo development.

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EXOSC10/Rrp6 is essential for the eight-cell embryo/morula transition

Cited by 8 publications

References 63 publications

Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice

Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice

DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice

RNA exosome ribonuclease DIS3 degrades Pou6f1 to promote mouse pre-implantation cell differentiation

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