Exosomal miR‐663b targets Ets2‐repressor factor to promote proliferation and the epithelial–mesenchymal transition of bladder cancer cells

Yin, Xinbao; Zheng, Xueping; Liu, Ming; Wang, Dong; Sun, Hui; Qiu, Yue; Chen, Jun; Shi, Benkang

doi:10.1002/cbin.11292

Cited by 29 publications

(25 citation statements)

References 28 publications

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“…Exosomal miR-663b circulates in the plasma of BCa patients. Overexpression of miR-663b promotes cell proliferation and tumor development at an significant level by targeting Ets2-repressor factor (ERF), while upregulating the ERF can eliminate the promotion function of miR-663b ( 52 ). Exosomal miR-4644 is another miRNA that promotes cell proliferation.…”

Section: The Biological Roles Of Exosome-derived Ncrnas In Bcamentioning

confidence: 99%

Biological Roles and Clinical Significance of Exosome-Derived Noncoding RNAs in Bladder Cancer

et al. 2021

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Bladder cancer (BCa) is a common heterogeneous urinary system tumor with high malignancy and limited advancement in treatment. Limited understanding of BCa has not contributed to any significant progress in diagnosis or treatment, exploring the mechanisms underlying BCa has become an urgent research focus. Exosomes, a type of extracellular vesicle (EV), have drawn substantial interest for their important roles in mediating intracellular communication. Exosomes shuttle numerous bioactive molecules, and noncoding RNAs (ncRNAs) are among the most numerous. ncRNAs including microRNA, long noncoding RNA, and circular RNA are sorted and packaged into exosomes selectively and transferred into recipient cells to regulate their function. Exosomal ncRNAs are associated with hallmarks of BCa, such as proliferation, apoptosis, epithelial-mesenchymal transition (EMT), cell cycle arrest, lymphangiogenesis, and chemotherapy resistance. Exosomal ncRNAs can also be detected in urine and serum, making them encouraging biomarkers for BCa diagnosis and prognosis. More importantly, exosomes exhibit excellent biocompatibility and potential for diversified applications. The delivery of bioactive substances and drugs into specific cells has become a promising approach for precision therapy for BCa patients. In addition, cancer vaccines have also received increasing attention. In this review, we summarize the current research on the regulatory roles of exosomal ncRNAs in BCa tumorigenesis and progression, as well as their potential clinical value in accelerating the diagnosis and therapy of BCa.

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Section: The Biological Roles Of Exosome-derived Ncrnas In Bcamentioning

confidence: 99%

Biological Roles and Clinical Significance of Exosome-Derived Noncoding RNAs in Bladder Cancer

et al. 2021

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“…Six miRNAs were co-predicted by all three databases [Figure 4A and 4B]. According to these results, circ_0049447 may act as a sponge for certain miRNAs whose validated functions include potential roles in GC development [19–37] [Table 2]. To further explore the underlying mechanisms, we detected the expression of these predicted miRNAs in MGC-803 cells overexpressed circ_0049447.…”

Section: Resultsmentioning

confidence: 88%

“…[16][17][18] Six miRNAs were co-predicted by all three databases [Figure 4A and 4B]. According to these results, circ_0049447 may act as a sponge for certain miRNAs whose validated functions include potential roles in GC development [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] [Table 2].…”

Section: Prediction Of Mirnas Bound By Circ_0049447mentioning

confidence: 95%

Circ_0049447 acts as a tumor suppressor in gastric cancer through reducing proliferation, migration, invasion, and epithelial-mesenchymal transition

Tang

Guo

et al. 2021

Chinese Medical Journal

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Background: Although increasing abnormal expression of circular RNAs (circRNAs) has been revealed in various cancers, there were a small number of studies about circRNAs in gastric cancer (GC). Here, we explored the expression and function of a novel circRNA, circ_0049447, in GC. Methods: A total of 80 GC tissues and non-tumorous tissues were collected from the First Affiliated Hospital of China Medical University. And all cells were cultured with 10% fetal bovine serum and incubated at 37°C and 5% CO 2 . The expression of circ_0049447 was quantified by real-time polymerase chain reaction. The biological function of circ_0049447 on proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) was evaluated by cell counting kit-8 (CCK-8), colony formation assay, transwell migration and invasion assay, and Western blotting. Luciferase report assay was used to verify the direct binding between circ_0049447 and predicted microRNA (miRNA). Furthermore, a xenograft mouse model was used to validate the function of circ_0049447 in vivo . Results: We demonstrated that circ_0049447 was downregulated in GC ( P < 0.001). The area under the receiver operating characteristic curve reached 0.838, while sensitivity was 82.3% and specificity was 77.2%. CCK-8 and colony formation assay showed that overexpression of circ_0049447 could inhibit the proliferation ( P < 0.05). Transwell migration and invasion assay showed upregulated circ_0049447 could impede migration in GC cells ( P < 0.05). In addition, overexpression of circ_0049447 could impede GC cell EMT. Upregulation of miR-324-5p in GC specimens and direct binding between miR-324-5p with circ_0049447 proven by luciferase reporter assay indicated that circ_0049447 may inhibit GC by sponging certain miRNA. Conclusion: Circ_0049447 acts as a tumor suppressor in GC through reducing proliferation, migration, invasion, and EMT, and it is a promising biomarker for diagnosis.

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“…In another study, exosomes derived from highly metastatic human oral cancer cells were found to transfer two onco-miRs, miR-1246 and miR-342-3p, to poorly metastatic cells at adjacent or distance sites and induced increased cell motility and invasive capacity [35]. Exosomal miRNAs such as miR-663b [36], miR-21 [37], miR-105 [38], miR181C [39], miR-106 [40], and miR-222 [41] and other lnc RNAs such as Sox2ot [42], ZFAS1 [43], and HOTTIP [44] promoted tumor migratory properties in several cancer types.…”

Section: The Protumorigenic Activity Of Exosomesmentioning

confidence: 98%

Bioengineering of Extracellular Vesicles: Exosome-Based Next-Generation Therapeutic Strategy in Cancer

et al. 2021

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Extracellular nano vesicles and exosomes hold compelling evidence in intercellular communication. Exosomal intracellular signal transduction is mediated by the transfer of cargo proteins, lipids, micro (mi)RNAs, long noncoding (lnc)RNAs, small interfering (si)RNAs, DNA, and other functional molecules that play a pivotal role in regulating tumor growth and metastasis. However, emerging research trends indicate that exosomes may be used as a promising tool in anticancer treatment. This review features a majority of the bioengineering applications of fabricated exosomal cargoes. It also encompasses how the manipulation and delivery of specific cargoes—noncoding RNAs (ncRNAs), recombinant proteins, immune-modulators, chemotherapeutic drugs, and other small molecules—may serve as a precise therapeutic approach in cancer management.

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Exosomal miR‐663b targets Ets2‐repressor factor to promote proliferation and the epithelial–mesenchymal transition of bladder cancer cells

Cited by 29 publications

References 28 publications

Biological Roles and Clinical Significance of Exosome-Derived Noncoding RNAs in Bladder Cancer

Biological Roles and Clinical Significance of Exosome-Derived Noncoding RNAs in Bladder Cancer

Circ_0049447 acts as a tumor suppressor in gastric cancer through reducing proliferation, migration, invasion, and epithelial-mesenchymal transition

Bioengineering of Extracellular Vesicles: Exosome-Based Next-Generation Therapeutic Strategy in Cancer

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