Exosome membrane-modified M2 macrophages targeted nanomedicine: Treatment for allergic asthma

Pei, Wenjun; Li, Xueqin; Bi, Runlei; Zhang, Xin; Zhong, Min; Yang, Hui; Zhang, Yingying; Lv, Kun

doi:10.1016/j.jconrel.2021.08.024

Cited by 69 publications

(36 citation statements)

References 24 publications

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“…EVs might have homing effects on their original cells or tissues. A recent study showed that M2-EVs exhibited a tropism effect on M2 cells in a mouse model of allergic asthma [ 52 ], suggesting that Mφ-EVs may be favorable for macrophage-related immune diseases. As a type of cell-derived nanomaterial, the therapeutic potential of M2-EVs can be readily and precisely improved by genetic and chemical modifications, and loading with specific therapeutic reagents in response to different disease conditions.…”

Section: Resultsmentioning

confidence: 99%

Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections

Wang

Liu²,

Li³

et al. 2022

Journal of Controlled Release

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Section: Resultsmentioning

confidence: 99%

Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections

Wang

Liu²,

Li³

et al. 2022

Journal of Controlled Release

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“…20 Exosomes have been emerging as important players in allergies and autoimmune diseases due to their ability to modulate both the innate and adaptive immunity responses, and may have potential as diagnostic and therapeutic tools. 21,22 A number of recent studies have revealed multiple regulatory effects of exosomes on KCs. Zhang et al demonstrated that exosomes in the plasma of The exosomes promoted intrinsic apoptosis of these keratinocytes via down-regulation of the X-linked inhibitor of apoptosis protein (XIAP), a key apoptotic regulator in these cells.…”

Section: Discussionmentioning

confidence: 99%

Plasma Exosomes from Children with Atopic Dermatitis May Promote Apoptosis of Keratinocytes and Secretion of Inflammatory Factors in vitro

Zhu¹,

Sun²,

Ma³

et al. 2022

CCID

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Purpose Exosomes are important regulators of keratinocytes (KCs) that have been implicated in a variety of skin disorders. The effect of circulatory exosomes on KCs in pediatric atopic dermatitis (AD) has not been well studied. This study aims to explore the effect of plasma exosomes on KC activation, apoptosis and inflammation in pediatric AD patients. Patients and Methods Exosomes were extracted from plasma collected from 20 pediatric AD patients and 20 age-matched healthy controls. AD-exosomes were added with KCs at concentrations of 0 g/L, 10 g/L, 20 g/L and 30 g/L. Proliferation of KCs in each group was measured using Ki67 staining flow cytometry. Apoptosis was measured using Annexin V-FITC/PI double staining flow cytometry. KCs were divided into three groups according to the source of the exosomes they were cultured with: patients with AD, healthy controls and blank controls. Q-PCR was used to detect the activation (K6) and differentiation (K10) of cells, as well as inflammatory indicators (thymic stromal lymphopoietin (TSLP) and IL-33). Results The proliferation rate of KCs treated with 20 g/L exosomes from AD patients was significantly lower than that of other groups, while the apoptosis rate was significantly increased. Additionally, expression levels of K6, K10, TSLP and IL-33 were all up-regulated compared to keratinocytes treated with exosomes from healthy controls. Conclusion Exosomes from the peripheral blood of pediatric AD patients can regulate the activation, apoptosis and inflammatory cytokine secretion of KCs in vivo, which may participate in the pathogenesis of AD.

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“…Based on this, a nanocomplex composed of smart silencer of Dnmt3aos (Dnmt3aos smart silencer ) encapsulated polylactic-co-glycolic acid (PLGA) core and EV membrane of M2 macrophages shell was exploited. M2 macrophages EV membrane endowed nanocomplex with the ability to achieve targeted delivery of Dnmt3aos smart silencer to injured lung tissue when PLGA improved the stability of nanocomplex thereby effectively delayed AA progression [ 86 ].…”

Section: Lung-related Diseasementioning

confidence: 99%

Tailored Extracellular Vesicles: Novel Tool for Tissue Regeneration

Qian

et al. 2022

Stem Cells International

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Extracellular vesicles (EVs) play an essential part in multiple pathophysiological processes including tissue injury and regeneration because of their inherent characteristics of small size, low immunogenicity and toxicity, and capability of carrying a variety of bioactive molecules and mediating intercellular communication. Nevertheless, accumulating studies have shown that the application of EVs faces many challenges such as insufficient therapeutic efficacy, a lack of targeting capability, low yield, and rapid clearance from the body. It is known that EVs can be engineered, modified, and designed to encapsulate therapeutic cargos like proteins, peptides, nucleic acids, and drugs to improve their therapeutic efficacy. Targeted peptides, antibodies, aptamers, magnetic nanoparticles, and proteins are introduced to modify various cell-derived EVs for increasing targeting ability. In addition, extracellular vesicle mimetics (EMs) and self-assembly EV-mimicking nanocomplex are applied to improve production and simplify EV purification process. The combination of EVs with biomaterials like hydrogel, and scaffolds dressing endows EVs with long-term therapeutic efficacy and synergistically enhanced regenerative outcome. Thus, we will summarize recent developments of EV modification strategies for more extraordinary regenerative effect in various tissue injury repair. Subsequently, opportunities and challenges of promoting the clinical application of engineered EVs will be discussed.

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Exosome membrane-modified M2 macrophages targeted nanomedicine: Treatment for allergic asthma

Cited by 69 publications

References 24 publications

Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections

Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections

Plasma Exosomes from Children with Atopic Dermatitis May Promote Apoptosis of Keratinocytes and Secretion of Inflammatory Factors in vitro

Tailored Extracellular Vesicles: Novel Tool for Tissue Regeneration

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