Exosomes and CAFs: partners in crime

Apollonio, Benedetta; Ramsay, Alan G.

doi:10.1182/blood-2015-07-655233

Cited by 6 publications

(4 citation statements)

References 10 publications

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“…In addition, bone marrow mesenchymal stem cells (BM-MSCs) play a critical role in maintaining normal hematopoiesis and the process of mobilization and homing, and in drug resistance and clinical recurrence, by secreting various cytokines, chemokines, and signaling molecules [10]. It is commonly acknowledged that exosomes are secreted by a variety of cells such as tumor cells and can induce the tumor microenvironment (TME) to support tumor growth and metastasis [11,12]. AML cells remodel the bone marrow niche leukemia-permissive microenvironment and inhibit hematopoiesis by deriving exosomes to BM-MSCs [13].…”

Section: Introductionmentioning

confidence: 99%

Hyperleukocytic Acute Leukemia Circulating Exosomes Regulate HSCs and BM-MSCs

Yang

et al. 2021

Journal of Healthcare Engineering

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Hyperleukocytic acute leukemia (HLAL) circulating exosomes are delivered to hematopoietic stem cells (HSCs) and bone marrow mesenchymal stem cells (BM-MSCs), thereby inhibiting the normal hematopoietic process. In this paper, we have evaluated and explored the effects of miR-125b, which is carried by HLAL-derived exosomes, on the hematopoietic function of HSCs and BM-MSCs. For this purpose, we have isolated exosomes from the peripheral blood of HLAL patients and healthy volunteers. Then, we measured the level of miR-125b in exosomes cocultured exosomes with HSCs and BM-MSCs. Moreover, we have used miR-125b inhibitors/mimic for intervention and then measured miR-125b expression and colony forming unit (CFU). Apart from it, HSC and BM-MSC hematopoietic-related factors α-globulin, γ-globulin, CSF2, CRTX4 and CXCL12, SCF, IGF1, and DKK1 expression were measured. Evaluation of the miR-125b and BAK1 targeting relationship, level of miR-125b, and expression of hematopoietic-related genes was performed after patients are treated with miR-125b mimic and si-BAK1. We have observed that miR-125b was upregulated in HLAL-derived exosomes. After HLAL-exosome acts on HSCs, the level of miR-125b is upregulated, reducing CFU and affecting the expression of α-globulin, γ-globulin, CSF2, and CRCX4. For BM-MSCs, after the action of HLAL-exo, the level of miR-125b is upregulated and affected the expression of CXCL12, SCF, IGF1, and DKK1. Exosomes derived from HLAL carry miR-125b to target and regulate BAK1. Further study confirmed that miR-125b and BAK1mimic reduced the expression of miR-125b and reversed the effect of miR-125b mimic on hematopoietic-related genes. These results demonstrated that HLAL-derived exosomes carrying miR-125b inhibit the hematopoietic differentiation of HSC and hematopoietic support function of BM-MSC through BAK1.

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Section: Introductionmentioning

confidence: 99%

Hyperleukocytic Acute Leukemia Circulating Exosomes Regulate HSCs and BM-MSCs

Yang

et al. 2021

Journal of Healthcare Engineering

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“…3 Cancer cell-derived exosomes have been shown to modulate the characteristics of the recipient cells by transferring chemoresistance, increase the metastatic potential, and enable stromal cell transformation to cancer-associated fibroblasts. 4,5 Interestingly, it was recently shown that exosomes secreted from the BMSCs support the growth and resistance to therapy of MM cells. 6,7 In addition to their functional properties upon uptake by recipient cells, exosomes were recently shown to possess intrinsic biological activity.…”

Section: Introductionmentioning

confidence: 99%

Caspase-3–dependent cleavage of Bcl-xL in the stroma exosomes is required for their uptake by hematological malignant cells

Vardaki

Sanchez

Fonseca

et al. 2016

Blood

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“…The main mechanism used by the tumor cell to generate phenotypic change is cell-to-cell interaction through exosomes, sometimes called "friends of crime", which are vesicles that contain miRNA, RNA and proteins whose mission is to generate a change in the function of the target cell in order to promote an inflammatory environment from which the tumor cell will benefit [31].…”

Section: Discussionmentioning

confidence: 99%