Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Accelerate Cutaneous Wound Healing by Enhancing Angiogenesis through Delivering Angiopoietin-2

Liu, Jinwen; Zhang, Yan; Yang, Fuji; Huang, Yuandong; Yu, Yao; Zhou, Liping; Sun, Zixuan; Cui, Dawei; Yan, Yongmin

doi:10.1007/s12015-020-09992-7

Cited by 84 publications

(60 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One example is the paracrine secretion of Angiopoietin 2 (Ang-2) [28]. Ang-2 is known to be secreted by some fibroblasts [29] and human mesenchymal stem cells [30]. The effect of co-cultures of these cells with the glycocalyx MVN in microfluidic cultures seems not to have been investigated so far.…”

Section: Resultsmentioning

confidence: 99%

Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays

Beyer

Blocki

Cheung

et al. 2021

Life

View full text Add to dashboard Cite

The endothelial glycocalyx forms the inner-most lining of human microvasculature. It ensures the physiological function of blood vessels and plays a crucial role in the occurrence and progression of microvascular diseases. The present communication aims to highlight the usefulness of high-resolution imaging of lectin (Bandeiraea Simplicifolia) stained endothelial glycocalyx in 3-dimensional microfluidic cell cultures. The microfluidic system allowed visualizing cancer cell extravasation, which is a key event in metastasis formation in cancer pathologies. In brief, microvascular networks were created through spontaneous vasculogenesis. This occurred from 3 dimensional (3D) suspensions of human umbilical vein endothelial cells (HUVECs) in hydrogels confined within microfluidic devices. Extravasation of MDA-MB-231 breast cancer cells from perfusable endothelial lumens was observed with confocal imaging of lectin-stained microvascular networks. The present work provides guidance towards optimizing the methodology used to elucidate the role of the endothelial glycocalyx during cancer cell extravasation. In particular, a high-resolution view of the endothelial glycocalyx at the site of extravasation is presented. The occurrence of glycocalyx defects is well aligned with the contemporary notion in the field that glycocalyx shedding precedes cancer cell extravasation.

show abstract

Section: Resultsmentioning

confidence: 99%

Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays

Beyer

Blocki

Cheung

et al. 2021

Life

View full text Add to dashboard Cite

show abstract

“…The study demonstrated the capability of EVs to stimulate angiogenesis through the transfer of DMTB1 protein to ECs [ 165 ]. Liu with colleagues determined that human umbilical cord MSC (HUMSC)-derived EVs promote cutaneous wound healing in rats after second-degree burns [ 166 ]. Data showed that after such EV application in vivo, the new epidermis was regenerated without a scar, and new vessels were nicely formed in the injury area compared to much worse results in the untreated control.…”

Section: Stem Cell-derived Extracellular Vesicles In Skin Wound Healingmentioning

confidence: 99%

“…Ang-1 participates in vessel stabilization, while Ang-2 regulates the interaction with cell-matrix by binding to integrin in new vessel growing. These biomolecules modulate angiogenesis activities via angiopoietin/TIE signaling pathway [ 166 ].…”

Section: Stem Cell-derived Extracellular Vesicles In Skin Wound Healingmentioning

confidence: 99%

Extracellular Vesicles in Skin Wound Healing

et al. 2021

View full text Add to dashboard Cite

Each year, millions of individuals suffer from a non-healing wound, abnormal scarring, or injuries accompanied by an infection. For these cases, scientists are searching for new therapeutic interventions, from which one of the most promising is the use of extracellular vesicles (EVs). Naturally, EV-based signaling takes part in all four wound healing phases: hemostasis, inflammation, proliferation, and remodeling. Such an extensive involvement of EVs suggests exploiting their action to modulate the impaired healing phase. Furthermore, next to their natural wound healing capacity, EVs can be engineered for better defined pharmaceutical purposes, such as carrying specific cargo or targeting specific destinations by labelling them with certain surface proteins. This review aims to promote scientific awareness in basic and translational research of EVs by summarizing the current knowledge about their natural role in each stage of skin repair and the most recent findings in application areas, such as wound healing, skin regeneration, and treatment of dermal diseases, including the stem cell-derived, plant-derived, and engineered EVs.

show abstract

“…Regarding the recovery of the vascular network, it has also been reported that MSC-derived exosomes can induce angiogenic activity in endothelial cells: (1) Human ASC-derived exosomes induce HUVEC tube formation by delivering miR-125a, thereby inhibiting the expression of the angiogenesis inhibitor delta-like 4[ 70 ]; (2) Human BM-MSC-EVs or rat BM-MSC-derived exosomes enhance angiogenesis in stroke mice[ 71 ] or in rats with renal ischemia-reperfusion (IR) injury[ 72 ]; (3) Exosomes from human endometrial MSCs can increase the expression of angiogenesis markers, including Tie2, vascular endothelial growth factor (VEGF), angiopoietin 1 (Ang1), and Ang2, and increase the proliferation, migration, and angiogenesis of human umbilical vein endothelial cells (HUVECs)[ 73 ]; and (4) In a rat burn model, treatment with hUCMSC-derived exosomes promoted Ang-2 protein expression in wounds and HUVECs through exosome-mediated Ang-2 transfer. The overexpression of Ang-2 in hUCMSC-derived exosomes further promoted the migration and tube formation of HUVECs, while knockout of Ang-2 in hUCMSC-derived exosomes eliminated these therapeutic and proangiogenic effects[ 74 ].…”

Section: Cutaneous Wound Healing By Msc-exosomesmentioning

confidence: 99%

Mesenchymal stem cell-derived exosomes: An emerging therapeutic strategy for normal and chronic wound healing

Zeng¹,

Liu²

2021

WJCC

View full text Add to dashboard Cite

Skin wound healing is a complex biological process. Mesenchymal stem cells (MSCs) play an important role in skin wound repair due to their multidirectional differentiation potential, hematopoietic support, promotion of stem cell implantation, self-replication, and immune regulation. Exosomes are vesicles with diameters of 40-100 nm that contain nucleic acids, proteins, and lipids and often act as mediators of cell-to-cell communication. Currently, many clinical scientists have carried out cell-free therapy for skin wounds, especially chronic wounds, using exosomes derived from MSCs. This review focuses on the latest research progress on the mechanisms of action associated with the treatment of wound healing with exosomes derived from different MSCs, the latest research progress on the combination of exosomes and other biological or nonbiological factors for the treatment of chronic skin wounds, and the new prospects and development goals of cell-free therapy.

show abstract

Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Accelerate Cutaneous Wound Healing by Enhancing Angiogenesis through Delivering Angiopoietin-2

Cited by 84 publications

References 55 publications

Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays

Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays

Extracellular Vesicles in Skin Wound Healing

Mesenchymal stem cell-derived exosomes: An emerging therapeutic strategy for normal and chronic wound healing

Contact Info

Product

Resources

About