Exosomes from human saliva as a source of microRNA biomarkers

Michael, Amanda; Bajracharya, Siddhartha; Yuen, Peter S.T.; Zhou, Hua; Star, Robert A.; Illei, Gabor G.; Alevizos, Ilias

doi:10.1111/j.1601-0825.2009.01604.x

Cited by 647 publications

(466 citation statements)

References 23 publications

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“…Exosomes also contain ESCRT-II subunits able to specifically bind mRNA. It was also discovered the presence of microRNAs within exosomes isolated from fresh and frozen glandular and whole human saliva [54] Recently, exosomes from human saliva have been characterized for their mRNA content and 509 mRNA were found. In vitro, exosomes were able to transfer their genetic information to human oral keratinocytes modifying gene expression.…”

Section: Rna Contentmentioning

confidence: 99%

Exosomes as intercellular signalosomes and pharmacological effectors

Record

Subra

Silvente-Poirot

et al. 2011

Biochemical Pharmacology

481

336

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International audienceCell secretion is a general process involved in various biological responses. Exosomes are part of this process and have gained considerable scientific interest in the past five years. Several steps through investigations across the last 20years can explain this interest. First characterized during reticulocyte maturation, they were next characterized as a key player in the immune response and cancer immunotherapy. More recently they were characterized as vectors of mRNAs, miRNAs and also lipid mediators able to act on target cells. They are the only type of vesicles released from an intracellular compartment from cells in viable conditions. They appear as a vectorized signaling system operating from inside a donor cell towards either the periphery, the cytosol, or possibly to the nucleus of target cells. Exosomes from normal cells trigger positive effects, whereas those from pathological ones, such as tumor cells or infected ones may trigger non-positive health effects. Therefore regulating the biogenesis and secretion of exosomes appear as a pharmacological challenge to intervene in various pathophysiologies. Exosome biogenesis and molecular content, interaction with target cells, utilisation as biomarkers, and functional effects in various pathophysiologies are considered in this review

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Section: Rna Contentmentioning

confidence: 99%

Exosomes as intercellular signalosomes and pharmacological effectors

Record

Subra

Silvente-Poirot

et al. 2011

Biochemical Pharmacology

481

336

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“…Extracellular vesicles (EVs) are found in the extracellular space as well as in almost all body fluids, including blood [1], urine [2], saliva [3] and cerebrospinal fluid [4]. EVs carry and protect cargoes, including proteins, nucleic acids and lipids, from their cells of origin [5,6] (Figure 1(a)).…”

Section: Introductionmentioning

confidence: 99%

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Guo

Tao

Dawn

2018

J of Extracellular Vesicle

150

111

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Extracellular vesicles (EVs), which can be found in almost all body fluids, consist of a lipid bilayer enclosing proteins and nucleic acids from their cells of origin. EVs can transport their cargo to target cells and have therefore emerged as key players in intercellular communication. Their potential as either diagnostic and prognostic biomarkers or therapeutic drug delivery systems (DDSs) has generated considerable interest in recent years. However, conventional methods used to study EVs still have significant limitations including the time-consuming and low throughput techniques required, while at the same time the demand for better research tools is getting stronger and stronger. In the past few years, microfluidics-based technologies have gradually emerged and have come to play an essential role in the isolation, detection and analysis of EVs. Such technologies have several advantages, including low cost, low sample volumes, high throughput and precision. This review summarizes recent advances in microfluidics-based technologies, compares conventional and microfluidics-based technologies, and includes a brief survey of recent progress towards integrated “on-a-chip” systems. In addition, this review also discusses the potential clinical applications of “on-a-chip” systems, including both “liquid biopsies” for personalized medicine and DDS devices for precision medicine, and then anticipates the possible future participation of cloud-based portable disease diagnosis and monitoring systems, possibly with the participation of artificial intelligence (AI).

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“…It has been shown that placental-specific miRNAs, released via exosomes, can be exported from the placental trophoblast into the maternal circulation, where they can be found abundantly. MicroRNAs have been found within exosomes isolated from human saliva (Michael et al , 2010 ). These exosomes may transfer their genetic information to human oral keratinocytes, thus modifying gene expression in the cell.…”

Section: Nucleic Acidsmentioning

confidence: 99%

Exosomes: the ideal nanovectors for biodelivery

Fais

Logozzi

Lugini

et al. 2012

Biological Chemistry

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Nanomedicine aims to exploit the improved and often novel physical, chemical, and biological properties of materials at the nanometric scale, possibly with the highest level of biomimetism, an approach that simulates what occurs in nature. Although extracellularly released vesicles include both microvesicles (MVs) and exosomes, only exosomes have the size that may be considered suitable for potential use in nanomedicine. In fact, recent reports have shown that exosomes are able to interact with target cells within an organ or at a distance using different mechanisms. Much is yet to be understood about exosomes, and currently, we are looking at the visible top of an iceberg, with most of what we have to understand on these nanovesicles still under the sea. In fact, we know that exosomes released by normal cells always trigger positive effects, whereas those released by cells in pathological condition, such as tumor or infected cells, may induce undesired, dangerous, and mostly unknown effects, but we cannot exclude the possibility that exosomes may also be detrimental for the body in normal conditions. However, whether we consider extracellular vesicles as a whole, thus including MVs, it appears that even in normal conditions, extracellular vesicles may lead to unwanted effects, depending on gender and age. This review aims to critically emphasize existing data in the literature that support the possible roles of exosomes in both diagnostic and therapeutic scopes.

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Exosomes from human saliva as a source of microRNA biomarkers

Cited by 647 publications

References 23 publications

Exosomes as intercellular signalosomes and pharmacological effectors

Exosomes as intercellular signalosomes and pharmacological effectors

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Exosomes: the ideal nanovectors for biodelivery

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