Exosomes participate in the carcinogenesis and the malignant behavior of gastric cancer

Yan, Yongmin; Fu, Guangzhen; Ye, Yafei; Ming, Liang

doi:10.1080/00365521.2016.1278458

Cited by 48 publications

(39 citation statements)

References 69 publications

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“…Interestingly, some circRNAs exhibit a strong degree of tumor tissue specificity and are even associated with important clinical features, including tumor stage, grade, metastasis, and recurrence [50,51]. Additionally, circRNAs in exosomes secreted by tumor cells can be transferred to normal cells, indicating that exo-circRNAs are important in the peritoneal metastasis of tumors [52].…”

Section: Discussionmentioning

confidence: 99%

hsa_circ0021347 as a Potential Target Regulated by B7-H3 in Modulating the Malignant Characteristics of Osteosarcoma

Wang

Zhang

Kang

et al. 2019

BioMed Research International

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In our previous study, we showed that B7-H3 played crucial roles in osteosarcoma (OS) development and might serve as a negative regulator of in osteoimmunology and help tumor cells escape immune surveillance. However, little is known about B7-H3 deficiency and its corresponding circRNA alteration or their relationship with osteosarcoma progression. erefore, we established stable silencing of B7-H3 in OS cells and validated our results with western blotting and real-time PCR detection. en, we performed a circRNA array to analyze the differential expression of circRNAs between the control and B7-H3 knockdown cells. e association between target circRNA expression and the clinicopathological features of patients with OS was further analyzed. As a result, hsa_circ0021347 was selected and validated to be significantly downregulated in OS tissues and cell lines and showed a strong negative relationship with B7-H3 expression in OS. In addition, clinicopathological features showed that hsa_circ0021347 in OS tissues was negatively associated with Enneking stage and positively associated with patients' survival. Finally, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and PANTHER pathway analyses were performed to predict a network of hsa_circ0021347/miRNAs interactions to help us develop potential biomarkers for clinical diagnosis and design therapeutic strategies for OS.

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Section: Discussionmentioning

confidence: 99%

hsa_circ0021347 as a Potential Target Regulated by B7-H3 in Modulating the Malignant Characteristics of Osteosarcoma

Wang

Zhang

Kang

et al. 2019

BioMed Research International

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“…Exosomes are small membrane vesicles ranging from 30 to 100 nm in size and play a critical role in intercellular communication by their various contents . There are increasing evidences that cancer‐derived exosomes may contribute to tumorigenesis and metastasis and served as a potential biomarker . Meanwhile, due to their presence in most body fluids, exosomes might sever as noninvasive clinical biomarkers .…”

Section: Discussionmentioning

confidence: 99%

“…57 There are increasing evidences that cancer-derived exosomes may contribute to tumorigenesis and metastasis and served as a potential biomarker. 58,59 Meanwhile, due to their presence in most body fluids, exosomes might sever as noninvasive clinical biomarkers. 60 Accordingly, we extracted exosomes from CL187 cell supernatant and CRC patient peripheral blood, and demonstrated that LEA/PODXL highly accumulated in the exosomes using ND-1-probed western blotting assay.…”

Section: Discussionmentioning

confidence: 99%

Identification of LEA, a podocalyxin‐like glycoprotein, as a predictor for the progression of colorectal cancer

et al. 2018

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Large external antigen (LEA) is considered as a colorectal cancer (CRC)‐associated antigen, which was found via mAb ND‐1 generated using hybridoma technology, but its molecular features remain unknown. To facilitate the clinical application of LEA, we identified LEA as a podocalyxin‐like protein 1 (PODXL) with molecular weight of approximately 230 kDa, a hyperglycosylated protein, using immunoprecipitation and mass spectrometry in combination, and verified that ND‐1‐recognized epitope is on the terminal sialic acid of LEA. Correlation analysis between LEA and PODXL in molecular weight, immunological cross‐reactivity, and gene expression dependence supported the PODXL identity of the LEA. Moreover, we assessed the clinical significance of the LEA in 89 pairs of primary CRC tissues and adjacent nontumor colorectal tissues using ND‐1 by quantum dot‐based immunohistochemistry (QD‐IHC). High LEA expression was correlated significantly with T stage (P = 0.010). Patients with high LEA expression showed significantly poorer prognosis than those with LEA low expression (P = 0.007). Multivariate analysis indicated LEA expression as an independent predictor. Furthermore, the comparative analysis showed that mAb ND‐1‐based IHC analysis toward sugar residue of PODXL has higher sensitivity and specificity to evaluate the LEA/PODXL expression than mAb 3D3‐based method toward core protein of PODXL in CRC cell lines and clinical samples. In addition, we first found that LEA/PODXL can be secreted in exosomes from cancer cells and CRC patient peripheral blood. Our results demonstrate that LEA is an independent predictor for CRC progression and has the potential to be applied for clinical setting with high sensitivity, high specificity, and noninvasive access.

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“…In any case, it is clear that EVs can have deep effects on recipient cells, especially in pathological conditions [ 58 , 59 , 60 , 61 , 62 , 63 , 64 ]. Moreover, these cargoes can be at least partially transferred into the recipient cells, and there are examples (see below) of all the different categories of molecules that EVs carry with them.…”

Section: Extracellular Vesicles As Vehicles For the Horizontal Tramentioning

confidence: 99%

Extracellular Vesicle‐Associated RNA as a Carrier of Epigenetic Information

Schiera

Liegro

2017

Genes

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Post-transcriptional regulation of messenger RNA (mRNA) metabolism and subcellular localization is of the utmost importance both during development and in cell differentiation. Besides carrying genetic information, mRNAs contain cis-acting signals (zip codes), usually present in their 5′- and 3′-untranslated regions (UTRs). By binding to these signals, trans-acting factors, such as RNA-binding proteins (RBPs), and/or non-coding RNAs (ncRNAs), control mRNA localization, translation and stability. RBPs can also form complexes with non-coding RNAs of different sizes. The release of extracellular vesicles (EVs) is a conserved process that allows both normal and cancer cells to horizontally transfer molecules, and hence properties, to neighboring cells. By interacting with proteins that are specifically sorted to EVs, mRNAs as well as ncRNAs can be transferred from cell to cell. In this review, we discuss the mechanisms underlying the sorting to EVs of different classes of molecules, as well as the role of extracellular RNAs and the associated proteins in altering gene expression in the recipient cells. Importantly, if, on the one hand, RBPs play a critical role in transferring RNAs through EVs, RNA itself could, on the other hand, function as a carrier to transfer proteins (i.e., chromatin modifiers, and transcription factors) that, once transferred, can alter the cell’s epigenome.

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Exosomes participate in the carcinogenesis and the malignant behavior of gastric cancer

Abstract: Exosomes play a vital role in gastric cancer carcinogenesis and metastasis.

Cited by 48 publications

References 69 publications

hsa_circ0021347 as a Potential Target Regulated by B7-H3 in Modulating the Malignant Characteristics of Osteosarcoma

hsa_circ0021347 as a Potential Target Regulated by B7-H3 in Modulating the Malignant Characteristics of Osteosarcoma

Identification of LEA, a podocalyxin‐like glycoprotein, as a predictor for the progression of colorectal cancer

Extracellular Vesicle‐Associated RNA as a Carrier of Epigenetic Information

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