Flexible substrates play a vital role in the design of wearable antennas. Analysis and fabrication of rectangular microstrip patch antenna have been demonstrated in this paper on a least explored flexible PVC-based transparent sheet. The substrate’s dielectric constant and loss tangent have been calculated by conducting a standard ring resonator test. The effect of bending on an antenna with different bending diameters along the x-axis and y-axis has been observed on Vector Network Analyzer (VNA) to verify the flexibility of substrate material for wearable applications.