Experience in implementing major national projects to combat degradation and desertification in Russia

Kulik, K.N.; Vlasenko, M.V.

doi:10.1016/j.cscee.2023.100583

Case Studies in Chemical and Environmental Engineering

2024

DOI: 10.1016/j.cscee.2023.100583

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Experience in implementing major national projects to combat degradation and desertification in Russia

K.N. Kulik,

M.V. Vlasenko

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2024

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Cited by 3 publications

References 18 publications

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Assessment of degradation transformations of agricultural lands

Zhichkina,

Mirgorodskaya,

Zhichkin

et al. 2024

BIO Web Conf.

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The use of soil for the production of crop products leads to changes in the natural properties of soils and their natural state. When various situations arise, soil properties can change in different forms and with unequal degrees of severity; a process that worsens quantitative and qualitative properties is defined as soil degradation. The significant role of soil in people’s lives should serve as an incentive for its rational use and to prevent the occurrence of degradation transformations leading to a decrease in its environmental and production functions. The purpose of the research is to assess soil contamination of agricultural lands in the Samara region with insecto-acaricides and herbicides. Objectives: - to establish the average and maximum content of residual quantities of pesticides in soils; - determine the area of contamination with residual quantities of pesticides. The research was carried out in 7 districts (Bezenchuksky, Syzransky, Stavropolsky, Volzhsky, Privolzhsky, Bolshechernigovsky, Elkhovsky) of the Samara region in 2020-2022. To assess soil contamination with residual amounts of pesticides, soil samples were taken in spring and autumn to the depth of the arable layer. In selected soil samples, the residual amount of insectoacaricides (DDT, HCH, HCB, metaphos) and herbicides (prometrin, atrazine, simazine, 2.4-D, trifluralin, THAN, dalapon) was determined. The analysis of contamination of agricultural land in the study region showed that the content of controlled pesticides in soils depended on both the period of soil sampling and the year of research.

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Assessment of degradation transformations of agricultural lands

Zhichkina,

Mirgorodskaya,

Zhichkin

et al. 2024

BIO Web Conf.

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Selection of reference genes for quantitative gene expression analyses in pedunculate oak (Quercus robur L.) using real-time quantitative PCR

Zybinskaya,

Tretyakova

2024

E3S Web Conf.

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Drought and desertification are global issues leading to numerous negative consequences, such as reducing the arable land area, decreasing soil fertility, and crop yield. An effective method to fight against degradation and desertification of agricultural lands is agroforestry, thus there is a need to obtain drought-resistant tree species for use in agroforestry on arid lands. One of the key species used for creating forest strips is pedunculate oak (Quercus robur L.). Understanding the molecular mechanisms of adaptation to drought conditions is necessary to obtain resistant tree species. Real-time quantitative PCR method allows studying gene expression changes under various stress factors, but accurate results require careful selection of reference genes. The aim of this study is to select reference genes for quantitative gene expression analysis of Q. robur using RT-qPCR. Modeling of soil drought in the laboratory conditions was conducted to evaluate the stability of housekeeping genes expression in Q. robur. The RT-qPCR data were analyzed using the RefFinder web tool. Results from various algorithms showed that ACT7 had the most stable expression, while EF1α had the least stable expression. ACT7 is recommended as the reference gene for gene expression studies of Q. robur under drought conditions.

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Search for motifs, associated with response to abscisic acid in promoters of genes of Quercus robur and Populus trichocarpa plants

Malov,

Derevshchikova

2024

E3S Web Conf.

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Climate changing and increasing anthropogenic impact put more and more fertile lands under treat of degradation. To counteract this process, diverse methods, including agroforestry, should be applied, which, regarding changing environmental condition, requires development of new drought resistant tree forms and varieties. Quercus robur and Populus trichocarpa are actively used for this cause on the territories of southern Russia, their genomes are sequenced and annotated automatically, which facilitates search for potential genes of interest, which impact drought tolerance. Abscisic acid is a key participant of water deficiency and other abiotic stress response regulation, so genes, which expression is activated by it, are of interest as targets for further molecular selection and expression regulation researches. Promoter elements determining gene expression in response to this signal are already known. In the absence of experimental data about certain genes and their products, discovering such elements in their promoters allows to predict promising genes of interest with high probability. In this research, we conducted search for abscisic acid response elements ABRE and CE1 in promoters of genes of Quercus robur and Populus trichocarpa, and potential genes of interest were found.

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Experience in implementing major national projects to combat degradation and desertification in Russia

Cited by 3 publications

References 18 publications

Assessment of degradation transformations of agricultural lands

Assessment of degradation transformations of agricultural lands

Selection of reference genes for quantitative gene expression analyses in pedunculate oak (Quercus robur L.) using real-time quantitative PCR

Search for motifs, associated with response to abscisic acid in promoters of genes of Quercus robur and Populus trichocarpa plants

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