Experience with subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) on unfertilized aged human oocytes

Tsirigotis, M.; Bennett, Valerie; Nicholson, Natasha; Khalifa, Y.; Hogewind, Georg; Yazdani, Nahid; Craft, Ian

doi:10.1007/bf02211724

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…Percutaneous epididymal sperm aspiration (PESA) Percutaneous epididymal sperm aspiration for intracytoplasmic sperm injection was described in 1994 by Craft and colleagues. 124 It offers a relatively quick, minimally invasive and inexpensive method for sperm retrieval. Originally described in patients with OA, 125 it remains the first choice in that group due its minimally invasive nature.…”

Section: Microtesementioning

confidence: 99%

Surgical techniques for the management of male infertility

Lopushnyan¹,

Walsh²

2011

Asian J Androl

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Evaluation and surgical treatment of male infertility has evolved and expanded, now leading to more precise diagnoses and tailored treatments with diminished morbidity and greater success. Surgeries for male infertility are divided into four major categories: (i) diagnostic surgery; (ii) surgery to improve sperm production; (iii) surgery to improve sperm delivery; and (iv) surgery to retrieve sperm for use with in vitro fertilization and intracytoplasmic sperm injection (IVF-ICSI). While today we are more successful than ever in treating male infertility, pregnancy is still not always achieved likely due to factors that remain poorly understood. Clinicians treating infertility should advocate for couple-based therapy, and require that both partners have a thorough evaluation and an informed discussion before undergoing specific surgical therapies.

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Section: Microtesementioning

confidence: 99%

Surgical techniques for the management of male infertility

Lopushnyan¹,

Walsh²

2011

Asian J Androl

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“…Techniques of intracytoplasmic (ICSI) or subzonal (SUZI) sperm injection and intracytoplasmic cell injection (ICCI) are used to fertilize metaphase II and/or immature oocytes (Tsirigotis et al, 1994;Yanagimachi, 1995 andTarín, 1995). Transfer of male germ or embryonic or somatic nuclei into MII or GV cytoplasts might enable creating the new type of oocytes carrying the haploid introduced nuclear genome of male through meiotic maturation.…”

Section: Intracytoplasmic and Subzonal Sperm Injection And Intracytoplasmic Cell Injectionmentioning

confidence: 99%

Assisted Reproductive Technology in Mammals

2011

Egyptian Journal of Animal Production

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This review describes the use of modern reproductive biotechnologies or assisted reproductive techniques (ART) including artificial insemination, embryo transfer, in vitro fertilization, gamete/embryo micromanipulation, semen sexing, genome resource banking, and somatic cell nuclear transfer (cloning) for mammalian species. Such biotechnologies allow more offspring to be obtained from selected parents to increase productivity of milk and meat, reduce the interval between generations, therapy of diseases and conservation of endangered mammalian species. Practically, current reproductive biotechnologies are species-specific because of differences in estrous cycle, seasonality, structural anatomy, gamete physiology and site for semen deposition or method of embryo transfer.

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“…One-day-old reinsemination [1,2], or 1-day-old-ICSI [3][4][5][6][7] may be performed when in vitro fertilization (IVF) completely fails to achieve fertilization in some patients. In addition, when oocytes collected for intracytoplasmic sperm injection (ICSI) turn out to be immature oocytes, ICSI is sometimes performed on in vitro matured oocytes.…”

mentioning

confidence: 99%

Spindle and Chromosome Abnormality of Aged, Matured Oocytes in Human

et al. 2002

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There are cases in which the 1-day-old unfertilized oocytes after IVF, or in vitro matured oocytes obtained from denuded immature oocytes after ICSI, are used clinically. We investigated the morphological change of metaphase II spindle and chromosomal alignment in those oocytes. The spindles and chromosome were stained using an anti-α-tubulin antibody and Hoechst 33258 respectively. One-day-old oocytes displayed significant increases in abnormalities o f s p i n d l e s t r u c t u r e ( 4 0 . 4 % v s . 1 7 . 6 % ) a n d chromosomal alignment (40.4% vs. 29.4%). Oocytes matured in vitro from MI and PI oocytes displayed spindle morphological abnormalities at the rates of 41.7% and 36.0% respectively, and chromosomal alignment abnormality at the rates of 45.8% and 44.0% respectively. These results suggest that 1-day-old oocytes and in vitro matured oocytes obtained from denuded immature oocytes after ICSI might have lowered developmental potential. More than 50% of those oocytes were determined to be unsuitable for use as gametes.

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Experience with subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) on unfertilized aged human oocytes

Cited by 5 publications

References 24 publications

Surgical techniques for the management of male infertility

Surgical techniques for the management of male infertility

Assisted Reproductive Technology in Mammals

Spindle and Chromosome Abnormality of Aged, Matured Oocytes in Human

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