Experiences with Freund's complete adjuvant test (FCAT) when screening for contact allergens in colophony

Boman, Anders; Karlberg, Ann‐Therése; Wahlberg, J.E.

doi:10.1111/j.1600-0536.1988.tb05485.x

Cited by 36 publications

(22 citation statements)

References 16 publications

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“…The organic layers were washed with a saturated solution of NaHC03 (3 X 25 mL) and then with water (25 mL), dried over MgSO4, filtered, and evaporated under reduced pressure to give the crude epoxide, which was purified by column chromatography (20% AcOEt/hexane) to give 1.00 g (91 % yield) of 3 as a colorless oil. 1H NMR (CDC13) 6 1.35 ( Closed challenge testing was used according to previous experience (11). For induction the animals received intradermal injections (0.1 mL of 3% solution in FCA/H20 emulsion) in the upper back on days 0,6, and 10.…”

Section: ]Octan-4-ol(4)mentioning

confidence: 99%

Interactions of Allergenic Hydroperoxides with Proteins: A Radical Mechanism?

Lepoittevin¹,

Karlberg²

1994

Chem. Res. Toxicol.

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1-(1-Hydroperoxy-1-methylethyl)cyclohexene was synthesized as a model compound for the study of the interaction of 15-hydroperoxyabietic acid-like terpenes with proteins. Two related epoxides, 1-(1-hydroxy-1-methylethyl)-2-oxabicyclo[4.1.0]heptane and 2,2-dimethyl-1-oxaspiro-[2.5]octan-4-ol, were also prepared as reference materials. Treatment of the hydroperoxide with FeCl3 and N alpha-Ac-Cys-OMe led to the formation of the corresponding alcohol and of both epoxides. The allergenic activity of these compounds was tested in guinea pigs using the Freund's complete adjuvant test. The hydroperoxide was found to be a strong sensitizer while both epoxides were found to be inactive at the same doses. The generation of highly-reactive radicals in the epidermis could lead to the formation of antigenic structures, the first step of the allergic contact dermatitis mechanism.

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Section: ]Octan-4-ol(4)mentioning

confidence: 99%

Interactions of Allergenic Hydroperoxides with Proteins: A Radical Mechanism?

Lepoittevin¹,

Karlberg²

1994

Chem. Res. Toxicol.

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“…The FCAT procedure was performed m the modified version (9) according to our earlier expenence (10). 3 mtradermal InJeCtiOns of a 5% solution of hydrogenated rosm m Freund's complete adjuvant (FCA) were used for mduction.…”

Section: Freund's Complete Adjuvant Test ( Fcat)mentioning

confidence: 99%

Hydrogenation reduces the allergenicity of colophony (rosin)

Karlberg¹,

Boman²,

Nilsson³

1988

Contact Dermatitis

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Abietic acid is the main component of rosin. It is readily oxidized by air and its oxidation products are considered to be mainly responsible for the allergenic effect. Hydrogenation of the conjugated double bonds of abietic acid decreases its susceptibility to air oxidation and would thus reduce the allergenicity of rosin. Portuguese gum rosin was therefore hydrogenated and its allergenicity was compared with that of unmodified rosin in animal experiments and by patch testing in humans. Its sensitizing potential was determined in 2 studies. No response was found according to the FCAT method while the GPMT gave significant response in the animals challenged with the highest test concentration. Hydrogenated rosin showed no eliciting activity in animals induced with unmodified rosin according to the GPMT method. A marked decrease was found in the frequency of allergic reactions to hydrogenated rosin compared to the reactions to unmodified rosin in patients with known allergy to gum rosin. We conclude that elimination of the unsaturated non-aromatic compounds by hydrogenation considerably reduces the allergenicity of Portuguese gum rosin.

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“…18,19 At induction the animals received an occlusive epidermal application on the shaved upper back on days 0, 2, 7, and 9. About 200 mg of the test material was applied on pieces of filter paper (4 × 2 cm) at each of the four inductions.…”

Section: Introductionmentioning

confidence: 99%

Atmospheric oxidation of poly(oxyethylene) alcohols. Identification of ethoxylated formates as oxidation products and study of their contact allergenic activity

Bergh

Shao

Magnusson

et al. 1999

Journal of Pharmaceutical Sciences

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Ethoxylated alcohols are widely used as surfactants. In the present study we have continued our investigations on the degradation with time upon air exposure of the ethoxylated alcohols at normal storage and handling. As a result, a new group of ethoxylated formates with the general formula C12H25(OCH2CH2)nOCHO (n = 0-4) was identified in C12H25(OCH2CH2)5OH stored and handled at room temperature. To facilitate the identification work, reference compounds were synthesized. The formates showed no allergenic activity in the sensitization studies performed. In previous investigations on the same ethoxylated alcohol, we have identified formaldehyde and ethoxylated aldehydes among the oxidation products formed. Formaldehyde is a common contact allergen, and the ethoxylated aldehydes were shown to have a sensitizing capacity of the same magnitude as formaldehyde. The instability of the ethoxylated alcohols and formation of oxidation products may give an allergenic contribution to hand eczema caused by work with water and surfactants. To investigate the clinical significance in man an appropriate diagnostic patch testing in exposed humans is required.

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Experiences with Freund's complete adjuvant test (FCAT) when screening for contact allergens in colophony

Cited by 36 publications

References 16 publications

Interactions of Allergenic Hydroperoxides with Proteins: A Radical Mechanism?

Interactions of Allergenic Hydroperoxides with Proteins: A Radical Mechanism?

Hydrogenation reduces the allergenicity of colophony (rosin)

Atmospheric oxidation of poly(oxyethylene) alcohols. Identification of ethoxylated formates as oxidation products and study of their contact allergenic activity

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