Experimental alterations of the bound state of cerebral lysosomal enzymes

Clendenon, Nancy R.; Komatsu, Takashi; Allen, Nicholas; Gordon, Wanda A.

doi:10.1212/wnl.21.2.103

Cited by 7 publications

(2 citation statements)

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“…In addition, there was also a small but significant (P<0.001)increase in both cerebrosides and sulfatides in gray matter. It is interesting to note that decreased aryl sulfatase A activity has been reported in mouse brain homogenates associated with vitamin E deficiency (17). Increases in cerebrosides and sulfatides have also been observed in the muscles of rabbits maintained on a vitamin E-deficient diet (16).…”

Section: Discussionmentioning

confidence: 99%

Effect of vitamin E deficiency on the lipid class and fatty acid composition of rat brain gray and white matter

Vatassery¹,

Berry²,

Younozai³

et al. 1976

Lipids

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Three-week old male Sprague-Dawley rats were placed on a control or vitamin E-deficient diet for 9 months. The total lipid and cholesterol contents of brain gray and white matter areas in the vitamin E-deficient group did not differ from controls. The concentration of cerebrosides was lower in white matter but higher in gray matter of deficient animals. However, sulfatide was significantly (P less than 0.001) higher in white and gray matter of deficient animals compared with controls. Lysolecithin was not found in vitamin E-deficient gray matter but was present in control gray matter lipids. No marked differences were found in the concentrations or relative amounts of sphingomyelin, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine, or phosphatidyl inositol in the phospholipids of gray or white matter of vitamin E-deficient rats as compared to controls. In addition, no remarkable differences were found in the fatty acid composition of total lipid extracts of gray or white matter from vitamin E-deficient rats when compared with controls.

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Section: Discussionmentioning

confidence: 99%

Effect of vitamin E deficiency on the lipid class and fatty acid composition of rat brain gray and white matter

Vatassery¹,

Berry²,

Younozai³

et al. 1976

Lipids

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“…Aliquots of the homogenates were incubated within 1 hour as described below [231. The total activities of these hydrolases do not change appreciably under the conditions of sample storage and homogenate preparation described above [3,4,15,20]. /5-Glucuronidase (/?-G) (EC 3.2.1.31) was determined by a modification [8] of the method of Talalay et al [22] utilizing phenolphthalein-/?-glucuronide as sub strate.…”

Section: Methodsmentioning

confidence: 99%

Lysosomal Acid Hydrolase Activities in the Lungs of Fetal, Neonatal, Adult, and Senile Mice

Traurig¹

1976

Gerontology

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The activities of four lysosomal acid hydrolases (LAH) in the lungs of two strains of mice changed significantly throughout the life cycle. In the C57B1/6J animals, acid phosphatase (AP) and β-glucuronidase (β-G) were maximally active during early neonatal life then gradually declined to the adult levels by 4–5 weeks of age. After reaching the adult level, acid phosphatase activities did not change significantly through 20 months of age. In contrast, β-G activities increased markedly with advanced age. N-Acetyl-β-D-glucosaminidase (GAD) activities did not change significantly during late fetal, neonatal or young adult stages but increased significantly with advancing age. In the lungs of the CFW animals, the increase in activities of β-G and GAD between young adult life and advanced age was highly significant, whereas there was no notable change in the activities of acid phosphatase or arylsul-fatase (AS). The specific activities of the hydrolases in the lungs of the C57B1/6J strain were quite similar to those in the lungs of the CFW strain. The activities of all four hydrolases were markedly elevated in two spontaneous adenomatous tumors found in the lungs of old mice. The data indicate that LAH play a significant role in lung growth and maturation, and in changes associated with aging.

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