Experimental and natural borna disease virus infections: presence of viral RNA in cells of the peripheral blood

Vahlenkamp, Th.; Enbergs, H.; Müller, Hermann

doi:10.1016/s0378-1135(00)00242-x

Cited by 31 publications

(21 citation statements)

References 44 publications

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“…Therefore, it is possible to consider that this case is not a laboratory contamination. The degree of genetic divergence (0-6.5%) between these BDV PCR products and the reference strains (strains V and He/80) is in accordance with those described in previous studies [5,33,59,62,65]. Furthermore, the comparison of BDV sequences from animals of the same or different species shows the same level of divergence whether in France or elsewhere [62].…”

Section: Bdv Infections In Francesupporting

confidence: 89%

Borna disease: current knowledge and virus detection in France

Dauphin¹,

Legay

Pitel

et al. 2002

Vet. Res.

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-For over two centuries, Borna disease (BD) has been described as a sporadically occurring infectious meningoencephalomyelitis affecting horses and sheep in Central Europe. Over the last decade, the BD epidemiology has been discussed. Firstly, its geographical distribution seems larger than what was previously thought. Secondly, the disease can affect a large number of warm-blooded animal species, including humans. The aetiological agent is the Borna disease virus (BDV), an enveloped, nonsegmented negative-stranded RNA virus classified in the new virus family Bornaviridae (Mononegavirales order). It can induce severe clinical signs of encephalitis with striking behavioural disturbances and may cause death. BDV genome has recently been detected in France in the blood and brain of several animal species (horses, bovines, foxes). meningoencephalomyelitis / Borna disease virus / epidemiology / diagnosisRésumé -La maladie de Borna : connaissances actuelles et détection du virus en France. La maladie de Borna est connue depuis plus de deux siècles en Europe centrale comme une méningoencé-phalomyélite d'origine infectieuse affectant les chevaux et les moutons. Depuis ces dix dernières années, l'épidémiologie de la maladie a été révisée puisque la répartition géographique de la maladie de Borna s'avère plus large que rapportée jusqu'alors. En outre, elle peut affecter un grand nombre d'espèces animales à sang chaud, y compris l'homme. L'agent étiologique est le BDV (virus de la maladie de Borna), virus enveloppé, à ARN simple brin, non segmenté et de polarité négative, récem-ment classifié dans la nouvelle famille des Bornaviridae, (ordre des Mononegavirales). Le BDV peut induire des signes cliniques sévères d'encéphalite virale avec des troubles comportementaux importants et il peut entraîner la mort de l'animal. Récemment, le génome du BDV a été mis en évidence en France dans le sang et le cerveau de plusieurs espèces animales (chevaux, bovins, renards). méningoencéphalomyélite / virus de la maladie de Borna / épidémiologie / diagnostic 127

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Section: Bdv Infections In Francesupporting

confidence: 89%

Borna disease: current knowledge and virus detection in France

Dauphin¹,

Legay

Pitel

et al. 2002

Vet. Res.

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“…Whether the frequently observed absence of antibodies or their presence at low titers in naturally infected sheep (10,16,24,32) are due to the presence of plasma antigens and CIC has to be the focus of further investigations. Plasma antigenemia and CIC might also explain the detection of viral RNA in the absence of detectable antibodies, a condition found only in natural BDV infections (44).…”

Section: Discussionmentioning

confidence: 99%

“…The BDV p40-specific RT-PCR was performed as described recently (44). The BDV p24-specific primers used to investigate the swab and urine samples were BDV p24 s (5Ј-AGCTAGTGACGGAGCTGG-3Ј, nucleotides (nt) 1501 to 1518 [9]), BDV p24 as (5Ј-ATGCGCGGAGGTGCAGGA-3Ј, nt 1822 to 1805), BDV p24 nested s (5Ј-CGCATCGAGGCAGGGTTTGA-3Ј, nt 1578 to 1597), and BDV p24 nested as (5Ј-CGGCGGTTGATGCGTAGAGG-3Ј, nt 1773 to 1754).…”

Section: Animalsmentioning

confidence: 99%

“…The BDV p24-specific primers used to investigate the swab and urine samples were BDV p24 s (5Ј-AGCTAGTGACGGAGCTGG-3Ј, nucleotides (nt) 1501 to 1518 [9]), BDV p24 as (5Ј-ATGCGCGGAGGTGCAGGA-3Ј, nt 1822 to 1805), BDV p24 nested s (5Ј-CGCATCGAGGCAGGGTTTGA-3Ј, nt 1578 to 1597), and BDV p24 nested as (5Ј-CGGCGGTTGATGCGTAGAGG-3Ј, nt 1773 to 1754). Amplification was performed using the same temperature profile as described for the BDV p40-specific primers (44). The sensitivity for both the BDV-p24-specific (unpublished) and BDV-p40-specific (44) RT-PCR has been determined to be between 1 to 10 RNA molecules by the use of in vitro-synthesized RNA standards from different laboratories.…”

Section: Animalsmentioning

confidence: 99%

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Persistence of Borna Disease Virus in Naturally Infected Sheep

Vahlenkamp

Konrath

Weber

et al. 2002

J Virol

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“…Findings of BDV RNA and proteins in peripheral blood mononuclear cells indicate the possibility of hematogenous transmission (Solbrig et al, 2003;Vahlenkamp et al, 2000). Pisoni and colleagues reported the possible sexual transmission of BDV infection.…”

Section: The Transmission Of Bdv Infectionmentioning

confidence: 99%