Experimental and theoretical study on DPPH radical scavenging mechanism of some chalcone quinoline derivatives

Hamlaoui, Ikram; Bencheraiet, Reguia; Bensegueni, Rafik; Bencharif, Mustapha

doi:10.1016/j.molstruc.2017.11.118

Cited by 38 publications

(26 citation statements)

References 27 publications

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“…In brief, free Cur solution and Cur‐loaded NPs solution at 10 μg/mL (in ethanol) were suffered at 25ºC and 50ºC for 30 min. The scavenging activity assay was carried out by recording the absorbance of DPPH solution (100 μM) at 517 nm in the presence and absence of the remnant Cur with a UV‐vis spectrophotometer (Hamlaoui, Bencheraiet, Bensegueni, & Bencharif, ; Tonelli et al, ). The free radical scavenging potency of the curcumin was expressed as the percentage of DPPH that was decreased compared with that of the control condition after 30 min of preservation in the dark.…”

Section: Methodsmentioning

confidence: 99%

Curcumin encapsulation and protection based on lysozyme nanoparticles

Cui

Zhou

Huang

et al. 2019

Food Science & Nutrition

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Curcumin possesses antioxidant, anti‐inflammatory, and other properties. However, this compound exhibits low bioavailability because of its poor solubility and stability. In this paper, lysozyme nanoparticles were fabricated through solvent evaporation, and then, the solubilization and protection capability of curcumin were investigated. Lysozyme nanoparticles were characterized by dynamic light scattering technique, atomic force microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The load capacity and stability in thermal environment were further explored. Results showed that the lysozyme nanoparticle displayed a spherical structure (127.9 ± 2.12 nm) with favorable distribution. The solubility of curcumin can increase to 22 μg/mL. After encapsulation by lysozyme nanoparticles, the retentive curcumin can reach up to 67.9% and 30.25% at 25°C and 50°C, respectively, significantly higher than that of free curcumin. Meanwhile, experiments on DPPH free radicals indicated the curcumin loaded by lysozyme nanoparticle possessed higher free radical scavenging activity than that of free curcumin with same treatments. The results confirmed that lysozyme nanoparticles exhibit potential applications in solubilizing and protecting the environment‐sensitive hydrophobic functional components.

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Section: Methodsmentioning

confidence: 99%

Curcumin encapsulation and protection based on lysozyme nanoparticles

Cui

Zhou

Huang

et al. 2019

Food Science & Nutrition

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“…The antioxidant activity of the as‐loaded GA in the GA‐loaded CA fiber mats and films was evaluated by the DPPH assay. DPPH assay has been widely used to measure the antioxidant activity of various substances . DPPH radical (DPPH•) is a stable free radical that accepts an electron or hydrogen radical to become a stable molecule .…”

Section: Resultsmentioning

confidence: 99%

“…DPPH assay has been widely used to measure the antioxidant activity of various substances. 32,39,40 DPPH radical (DPPH•) is a stable free radical that accepts an electron or hydrogen radical to become a stable molecule. 12 GA can donate hydrogen radical and transform DPPH radical into its reduced form or nonradical form DPPH-H.…”

Section: Antioxidant Activity Of Ga-loaded Ca Fiber Matsmentioning

confidence: 99%

Gallic acid‐loaded electrospun cellulose acetate nanofibers as potential wound dressing materials

Wutticharoenmongkol

Hannirojram

Nuthong

2019

Polymers for Advanced Techs

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Gallic acid (GA)–loaded cellulose acetate (CA) nanofiber mats with 10 to 40 wt.% GA contents (based on the weight of CA) were fabricated by electrospinning. The effects of GA contents and applied potential on the morphology and the average diameters of fibers were studied. The electrospun fiber mats containing 20 and 40 wt.% GA were investigated for their potential use as carrier of GA in wound dressing application. The GA‐loaded CA films were prepared by solvent casting technique for use in comparative studies. Determination of the release characteristics of GA from the GA‐loaded fiber mats and films was carried out by the total immersion and the transdermal diffusion through a pig skin method in acetate buffer solution (pH 5.5) or normal saline (pH 7.0) at either 32 or 37°C, respectively. In the total immersion method, the maximum amounts of the GA released from the fiber mats containing 20 and 40 wt.% GA in the acetate buffer were approximately 97% and 71% (based on the weight of initial GA), while those of the GA released into the normal saline were approximately 96% and 81%, respectively. Lower values were observed in the experiments of the transdermal diffusion through a pig skin method. The corresponding GA‐loaded CA films showed the lower amounts of GA released into media. The as‐loaded and the as‐released GA remained its antioxidant activity as investigated by 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) assay. Lastly, the GA‐loaded CA fiber mats exhibited antibacterial activity against Staphylococcus aureus, which showed the potential for use as wound dressing materials.

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“…For antioxidant activity of phospholipid, most studies focused on the free radical scavenging ability, and there are few reports on the corresponding mechanism. Combining with antioxidant mechanisms, we speculated that unsaturated fatty acids converted into radicals at the first step, during which both hydrogen atom transfer and single‐electron transfer followed by proton transfer (SET‐PT) have been regarded as two main possible mechanisms (Hamlaoui, Bencheraiet, Bensegueni, & Bencharif, ; Maciel, Almeida, Da, & De, ), but futher exploration is still necessary for its corresponding antioxidant mechanism. DPPH, a stable nitrogen‐centered free‐radical containing asymmetric valence electrons (Hamlaoui et al., ), could be paired up with the electrons from unsaturated fatty acids to form reduced product (DPPH‐H) at the same time.…”

Section: Resultsmentioning

confidence: 99%

Egg Yolk Phosphatidylethanolamine: Extraction Optimization, Antioxidative Activity, and Molecular Structure Profiling

Sun

Chen

Bao

et al. 2019

Journal of Food Science

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Although phosphatidylethanolamine (PE) is an important functional phospholipid, there have been very few reports on its antioxidant activity and corresponding molecular composition. Crude PE was extracted from egg yolk with various solvents, and response surface modeling was carried out to determine the optimum extraction conditions for PE, under which the PE content in extracts reached 58.94 µg/mL. The crude PE was purified using silica gel-based column chromatography. High-purity PE (98%), identified by high-performance liquid chromatography-evaporative lightscattering detector, was obtained using isocratic elution with a mixed solvent (chloroform: methanol: acetic acid = 18:5:1) eluent. PE purified from egg yolk exhibited high radical-scavenging activity, determined by electron paramagnetic resonance (EPR). The result was attributed to the high unsaturated fatty acids (83.10%) content in egg yolk PE, and the unsaturated fatty acids were identified as PE-16:0/18:1 9 , PE-16:0/18:2 9,12 , 8,11,14 , PE-18:0/18:1 9 , PE-18:0/18:2 9,12 ,8,11,14 by MALDI-TOF MS combined with gas chromatography mass spectrometry.Practical Application: In this work, an attempt has been made to explore the antioxidant activity of PE that extracted and purified from egg yolk and its corresponding molecular composition. Owing to its plentiful unsaturated fatty acids (83.10%), purified PE from egg yolk exhibited a high radical-scavenging activity that indicated that egg-yolk PE had a strong antioxidant activity, and it might exert possible beneficial effects on the human health.

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Experimental and theoretical study on DPPH radical scavenging mechanism of some chalcone quinoline derivatives

Cited by 38 publications

References 27 publications

Curcumin encapsulation and protection based on lysozyme nanoparticles

Curcumin encapsulation and protection based on lysozyme nanoparticles

Gallic acid‐loaded electrospun cellulose acetate nanofibers as potential wound dressing materials

Egg Yolk Phosphatidylethanolamine: Extraction Optimization, Antioxidative Activity, and Molecular Structure Profiling

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