“…The applicant provided many publications at this respect. Some of these publications review the use of in vitro methods and their usefulness to study the digestion in dogs (Harmon, 2007 ; De Godoy et al, 2016 ; Deschamps et al., 2022 ); others provide data on in vitro studies only (Van Zelst et al, 2015 ; Lee et al, 2017 ; Kim et al., 2021 ) or do not allow for a full comparison of the results with those obtained in vivo (Gajda et al., 2005 ); a couple describe methods that may not be comparable to the one described by Boisen in 2007 (Smeets‐Peeters et al, 1999 ; Bosch et al, 2016 ); and some were developed from Boisen's or can be considered comparable but do not provide validation of in vivo reference values at pre‐caecal level (Dufour‐Etienne et al, 1992 ; Tonglet et al, 2001 ; Hervera et al, 2007 ; Hervera et al, 2009 ; Hooda et al, 2012 ; Biagi et al, 2016 ; Penazzi et al, 2021 ). Some of the latter showed good correlation between the digestibility coefficients in vitro and in vivo (faecal data); the conditions of the studies and diets evaluated may play a major role in the correlations observed.…”