Experimental Campylobacter jejuni infection in the chicken: an animal model of axonal Guillain-Barre syndrome.

Li, C Y; Xue, Ping; Tian, W Q; Liu, R C; Yang, Chuankai

doi:10.1136/jnnp.61.3.279

Cited by 67 publications

(53 citation statements)

References 21 publications

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“…In light of the wide occurrence of anti-GSL Abs in these patients, recent research on GBS has focused on GSL mimicry between LPSs in C. jejuni and endogenous GSLs in the peripheral nervous system that may be involved in the pathological mechanisms of GBS (Ang et al, 2004). Strain HS19 of C. jejuni was isolated from GBS patients and paralytic chickens (Li et al, 1996) and found to be associated with 29% of C. jejuniassociated GBS patients in the United States and 83% of cases in Japan (Nishimura et al, 1997;Allos et al, 1998). Although GSL mimicry as a causative mechanism remains uncertain for GBS and related diseases, the terminal tetrasaccharides Galβ1-3GalNAcβ1-4(NeuAcα2-3) Galβ1-4 of a GM1-like epitope and NeuAcα2-8NeuAcα2-3Galβ1-4Hep of a GD3-like epitope have been chemically determined in the LPSs from HS19 (Aspinall et al, 1992(Aspinall et al, , 1994Yuki et al, 1993).…”

Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

Molecular mimicry: Sensitization of Lewis rats with Campylobacter jejuni lipopolysaccharides induces formation of antibody toward GD3 ganglioside

Usuki

Thompson

Rivner

et al. 2005

J of Neuroscience Research

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Recently we have reported cases of demyelinating inflammatory neuropathy showing elevated titers of anti-GD3 antibodies, which occurs rarely in Guillain-Barré syndrome. To examine the correlation between the anti-GD3 antibody titer and Campylobacter jejuni infection, we sensitized female Lewis rats with lipopolysaccharides (LPSs) from serotype HS19 of C. jejuni and examined changes in nerve conduction velocity and nerve conduction block (P/D ratio). After 16 weeks of sensitization, animals revealed decreases of nerve conduction velocity and conduction block (P/D ratio) and high titer of anti-GD3 antibodies. These anti-GD3 antibodies also blocked transmission in neuromuscular junctions of spinal cord-muscle cells cocultures. The GD3 epitope was verified to be located on the Schwann cell surface and nodes of Ranvier in rat sciatic nerve. To determine the target epitope for GD3 antibodies in causing nerve dysfunction, the LPS fraction containing the GD3 epitope was purified from the total LPS by using an anti-GD3 monoclonal antibody-immobilized affinity column. Subsequently, chemical analysis of the oligosaccharide portion was performed and confirmed the presence of a GD3-like epitope as having the following tetrasaccharide structure: NeuAcα2-8NeuAc2-3Galβ1-4Hep. Our data thus support the possibility of a contribution of GD3 mimicry as a potential pathogenic mechanism of peripheral nerve dysfunction. Keywordsganglioside; Guillain-Barré; syndrome; anti-ganglioside antibody; GD3; Campylobacter jejuni; LPS Guillain-Barré syndrome (GBS) is an acute immune-mediated peripheral neuropathy consisting of acute inflammatory demyelinating polyneuropathy (AIDP), acute motor neuropathy (AMAN), and related disorders. Anti-GM1 antibodies are often detected in patients with AIDP and AMAN (Kornberg et al., 1994;Arasaki et al., 1998;Hiraga et al., 2005). Many investigators have reported the association between an antecedent Campylobacter jejuni (C. jejuni) infection and subsequent development of GBS. The lipopolysaccharides (LPSs) of a certain serotype of C. jejuni have been shown to possess carbohydrate epitopes similar to *Correspondence to: Dr. Robert K. Yu, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912-2697. ryu@mcg.edu. NIH Public Access NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript gangliosides, which may serve as the putative pathogenic triggers for subsequent GBS onset. Thus, these disorders may develop as a result of C. jejuni infection. In addition to anti-GQ1b Abs, anti-GD3 Abs have been detected in the Miller Fisher variant of GBS (Chiba et al., 1992;Carpo et al., 1998;Susuki et al., 2001;Willison and Yuki, 2002;Willison et al., 2004).Recently we have reported cases of AIDP and chronic inflammatory demyelinating polyneuropathy (CIDP) showing high elevations of anti-GD3 Abs, which rarely occurs in GBS (Usuki et al., 2005). The question then arises of the origin of the antiglycolipid (anti-GSL) Abs in GBS, in particular, the mechanism of el...

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Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

Molecular mimicry: Sensitization of Lewis rats with Campylobacter jejuni lipopolysaccharides induces formation of antibody toward GD3 ganglioside

Usuki

Thompson

Rivner

et al. 2005

J of Neuroscience Research

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“…Vriesendorp (1997) pointed out that the immune mechanisms by which infection with C. jejuni can create peripheral injury to axons instead of myelin should be studied. In this regard, Li et al (1996) were able to demonstrate development of paralysis in chickens infected with a strain of C. jejuni isolated from a human GBS patient who developed acute motor axonal neuropathy. One-third of the experimental chickens developed paralysis 5-18 days a�er oral administration of the C. jejuni culture.…”

Section: Gbs and C Jejunimentioning

confidence: 88%

Campylobacter jejuni: Public health hazards and potential control methods in poultry: a review

Hariharan¹,

Murphy²,

Kempf³

2004

Vet. Med. - Czech

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Certain strains of Campylobacter jejuni are implicated not only in diarrhea in humans, but also in the rare, but more serious Guillain-Barré syndrome, which may be fatal. Since poultry are the major reservoirs of C. jejuni, reducing contamination of poultry meat with this organism will decrease risk to the human consumer. Poultry meat which is contaminated with Campylobacter spp. and other human enteropathogens is safe for human consumption if handled properly while raw, and cooked completely. Recent experimental studies tend to indicate that diet formulations excluding animal proteins and fat may help towards reducing colonization of C. jejuni in the ceca of poultry, but attempts to combine this strategy with other methods including the use of probiotics aimed at competitive exclusion of C. jejuni, or prebiotics, which promote the growth of beneficial bacteria in the large intestine, and thereby reduce C. jejuni colonization is worth studying. Whether reduction of C. jejuni by these methods will cause proportionate increase of C. coli, which is emerging as a more drug resistant human pathogen, is not known. Colonization reduction should ideally be combined with innovative approaches at the processing plant to bring down contamination to a negligible level, although presently there is no “acceptable or safe quantitative level” for campylobacters in raw chicken meat.

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“…C. jejuni infection appears to be closely associated with the axonal forms of GBS as reported from studies carried out in China and Japan. Li et al [36] described C. jejuni induced neuritis in chickens mimicking acute axonal neuropathy as seen in human AMAN (acute motor axonal neuropathy) when fed with C. jejuni O:19. But this association is not consistent in western countries where the cases of AMAN with antecedent C. jejuni infection are less than 10%.…”

Section: Discussionmentioning

confidence: 99%

Automatic Evaluation of Test Strips for Anti-Ganglioside Antibodies in Patients with Guillain-Barré Syndrome Using EUROLineScan Software

Vaishnavi¹,

Behura²,

Prabhakar³

2014

AiM

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Campylobacter jejuni infection has been implicated in the pathogenesis of Guillain-Barré syndrome (GBS) due to production of humoral immune response against neural antigens. A case-control study was performed in a tertiary care teaching hospital for the estimation of anti-ganglioside antibodies in GBS patients and their controls. Blood samples were collected from 59 GBS cases, 58 neurological controls (NC) and 60 non-neurological control (NNC) patients for automatic estimation of IgG and IgM antibodies to seven gangliosides using EUROLineScan software. Antibodies of IgG class for GM1 were highly significant in GBS (p = 0.000) and NC (p = 0.031) compared to NNC. However GBS group was not significant (p = 0.413) compared to NC. For GM2 ganglioside, GBS and NC groups were significant (p = 0.000) compared to NNC, but GBS group was not significant (p = 0.999) compared to NC. For GM3 ganglioside, GBS and NC groups were significant (p = 0.000) compared to NNC; but GBS group was insignificant (p = 0.858) compared to NC with similar trend for all other ganglioside antibodies. When IgM class of antibodies was evaluated for GM1, GBS group was not significant (p = 0.604) whereas NC group was significant (p = 0.000) compared to NNC. GBS group was not significant compared to NC (p = 0.011). The trend was the same for GM2 antibodies. For GM3, GBS group was significant (p = 0.010) and NC was near significant (p = 0.055) compared to NNC. However GBS group was not significant (p = 0.808) compared to NC. No groups were significant (p > 0.05) in relation to the remaining gangliosides except for GQ1b where GBS group (p = 0.001) and NC group were significant (p = 0.000) compared to NNC. GBS group was also significant (p = 0.001) compared to NC and NNC. Anti-gangliosides antibodies were present in highly significant levels in the GBS group, though they were also present in the non-paralytic neurological control patients compared to the non-neurological control group.

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Experimental Campylobacter jejuni infection in the chicken: an animal model of axonal Guillain-Barre syndrome.

Abstract: Objective-To develop and characterise an animal model of paralytic neuropathy after Campylobacter jejuni infection.

Cited by 67 publications

References 21 publications

Molecular mimicry: Sensitization of Lewis rats with Campylobacter jejuni lipopolysaccharides induces formation of antibody toward GD3 ganglioside

Molecular mimicry: Sensitization of Lewis rats with Campylobacter jejuni lipopolysaccharides induces formation of antibody toward GD3 ganglioside

Campylobacter jejuni: Public health hazards and potential control methods in poultry: a review

Automatic Evaluation of Test Strips for Anti-Ganglioside Antibodies in Patients with Guillain-Barré Syndrome Using EUROLineScan Software

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Experimental Campylobacter jejuni infection in the chicken: an animal model of axonal Guillain-Barre syndrome.

Abstract: Objective-To develop and characterise an animal model of paralytic neuropathy after Campylobacter jejuni infection.

Cited by 67 publications

References 21 publications

Molecular mimicry: Sensitization of Lewis rats with Campylobacter jejuni lipopolysaccharides induces formation of antibody toward GD3 ganglioside

Molecular mimicry: Sensitization of Lewis rats with Campylobacter jejuni lipopolysaccharides induces formation of antibody toward GD3 ganglioside

Campylobacter jejuni: Public health hazards and potential control methods in poultry: a review

Automatic Evaluation of Test Strips for Anti-Ganglioside Antibodies in Patients with Guillain-Barr&#233; Syndrome Using EUROLineScan Software

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Product

Resources

About

Automatic Evaluation of Test Strips for Anti-Ganglioside Antibodies in Patients with Guillain-Barré Syndrome Using EUROLineScan Software