Experimental colitis is comorbid with social interaction deficits and anxiety‐like behaviors in mice: mechanistic intuitions into neuroinflammation and Claudin 5 expression in the hippocampus

Abstract

Inflammatory bowel disease (IBD) is accompanied by psychiatric disorders, including Schizophrenic‐like manifestations. Although incompletely illustrated, intestinal mucosal membrane damage and blood‐brain barrier (BBB) penetrability may have significant roles in psychiatric symptoms of IBD. This study aimed to investigate role of the Claudin‐5 (CLDN5) (a regulator of the permeability of BBB) and neuroinflammatory response in the comorbid behavioral disorders in experimental colitis in mice. Acetic acid was used to induce colitis in mice. 7 days after induction of colitis, behaviors including social interaction and locomotor activity as well as anxiety‐like behaviors were evaluated. Then, the colon was extracted for gross and microscopic evaluations. The expression of CLDN5, TNF‐α, IL1β and IL23 was measured by RT‐PCR in the colon and hippocampus. Histopathologic evaluations demonstrated mucosal, submucosal, and crypt‐related damages in the colon. The negative and positive number of social interactions significantly increased in the colitis group. A considerable increase in locomotor activities (horizontal and vertical components) shown in the colitis group. Mice in colitis group spent less time in the central zone in the open field apparatus. Gene expressions of TNF‐α, IL1β, and IL23 increased and CLDN5 decreased in the colitis group. The barrier function of the intestine and brain would be impaired, partially at least, following colitis (as we observed decrease in CLDN5 gene expression). Furthermore, we found that beside inflammatory response in the colon, a neuro‐immune response triggered in the hippocampus following colitis. These alterations probably, mediated comorbid behavioral disorders in acetic acid‐induced colitis in mice.