Experimental Concordant Xenografting in Small Animals

Kemp, E

doi:10.1007/978-3-642-72140-3_57

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…We transplanted metanephroi from an E15 Lewis rat embryo across a concordant xenogeneic barrier (21) into the peritoneum of 10 week‐old C57Bl/6 J mice. Two weeks later, either no trace of the metanephros could be found in mice, or a yellowish piece of tissue, too small to embed, was observed in the omentum.…”

Section: Xenotransplantation Of Embryonic Kidneysmentioning

confidence: 99%

Transplantation of embryonic organs - kidney and pancreas

Hammerman

2004

American Journal of Transplantation

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One novel solution to the shortage of human organs available for transplantation envisions 'growing' new organs in situ via xenotransplantation of developing anlagen from animal embryos. We and others have shown that renal anlagen (metanephroi) transplanted into animal hosts undergo differentiation and growth, become vascularized by blood vessels of host origin and exhibit excretory function. Metanephroi can be stored for up to 3 days in vitro prior to transplantation with no impairment in growth or function post-implantation. Metanephroi can be transplanted across both concordant (rat to mouse) and highly disparate (pig to rodent) xenogeneic barriers. Similarly, pancreatic anlagen undergo growth, differentiation and secrete insulin in a physiological manner following intraperitoneal placement. Implantation of the embryonic pancreas, is followed by selective differentiation of islet as compared to acinar components. Here we review studies exploring the potential therapeutic use of embryonic kidney or pancreas transplantation.

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Section: Xenotransplantation Of Embryonic Kidneysmentioning

confidence: 99%

Transplantation of embryonic organs - kidney and pancreas

Hammerman

2004

American Journal of Transplantation

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“…Concordant xenografting or xenotransplantation is transplantation of organs between closely related species, such as between primates or rodents. This definition was based on the difference in speed between hyperacute and acute rejection in allogeneic transplantation (Kemp, 1998). Johnsson et al described more than 100 days of survival of CsA treated concordant heart xenotransplants (Johnsson et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Composite tissue xenopreservation: Preliminary results of staged VCA in rat to mouse model

et al. 2023

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BackgroundThe time between procurement and transplantation of composite tissues, especially regarding the limited donor pool, is a challenge effecting the outcomes of the transplantation. Current preservation techniques mainly include either cold preservation with a solution or machine perfusion using blood or certain oxygen‐carrying solutions. However, none enables preservation beyond 24 h. Increasing this time to several days will provide better usage of the donor pool, safer transplantation of VCA with significant muscle content, and gives time to stabilize a patient before long surgical procedures. Herein, we described a novel strategy of xenopreservation (preservation via xenotransplantation) to preserve composite tissues for 7 days, followed by staged transplantation.Materials and MethodsWe used two concordant species, female Sprague Dawley rats (n = 10) and female CF‐1 mice (n = 10) in this study. Four of pair of animals are used for anatomical study. The groin flap of the rat was used as a xenograft and xenotransplanted to the neck area of the carrier mouse. Cyclosporine (CsA) was administered used as immunosuppressant. After 7 days of preservation on the mouse neck, xenotransplanted groin flap (called xenopreserved flap) was re‐harvested, skin and vessels samples were collected for histopathological evaluation, and the xenopreserved flap was transplanted to the donor rat's opposite groin area. Anastomoses were performed between the flap's pedicle and the femoral vessels. Clinical observation regarding inflammation and tissue perfusion of the xenopreserved flap was monitored daily. Fifteen days after the second surgical procedure, the rats were euthanized, and skin and vessel samples were collected. Histologic evaluation, including inflammatory cell numbers, was performed. Wilcoxon test was used to compare the changes in inflammation severity and p < .05 was set for statistical significance.ResultsAll xenopreserved groin flaps except one survived. Mean lymphocyte count before the second operation (at the end of the xenopreservation procedure) was 20,22 ± 0.44 and reduced to 13,14 ± 0.47 at the end of 15 days, and the difference was statistically significant (p < .05).ConclusionThis proof‐of‐concept study with preliminary results showed that xenotransplantation might be a novel strategy for preservation of VCA for a certain period of time. However, additional translational studies are needed to modulate the tissue changes following xenopreservation.

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Experimental Concordant Xenografting in Small Animals

Cited by 2 publications

References 12 publications

Transplantation of embryonic organs - kidney and pancreas

Transplantation of embryonic organs - kidney and pancreas

Composite tissue xenopreservation: Preliminary results of staged VCA in rat to mouse model

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