Experimental data-sets on the histopathological and immunohistological assessment of the Interrenal gland (adrenal homolog) of Japanese medaka (Oryzias latipes) fish exposed to graphene oxide

Evaluation of pancreatic δ- cells as a potential target site of graphene oxide toxicity in Japanese medaka (Oryzias latipes) fish

“…To verify the effects on endocrine pancreas, we hypothesized that the effects of GO on the islet organs of medaka are sex-specific and depends on the route of exposure. Due to the lack of any published report, for verification of our hypothesis, we have randomly selected some experimental fish from our previous experiments (IMR fish, Dasmahapatra and Tchounwou, 2022a ; b ; IP fish, Dasmahapatra et al, 2020a ; b ). IMR fish were exposed to GO by immersion, while in IP fish, GO was administered directly to the peritoneal cavity of the fish by injection ( Fig.…”

“…The procedure of GO synthesis, dilution, sonication, application to the animal was described previously ( Dasmahapatra et al, 2020a ; b , Dasmahapatra and Tchounwou, 2022a ; b ). Briefly, the GO used in the experiment was either synthesized in the laboratory and dispersed in the nanopure water at a concentration of 2 mg/mL ( Dasmahapatra et al, 2020a ; b ) or obtained from a commercial source (2 mg/mL dispersed in water; Sigma-Aldrich, St. Louis, MO, USA;).…”

“…Like IP experiments, the adult medaka fish we used for evaluation of pancreatic islet organs (endocrine pancreas) after IMR exposure were also obtained from our previous GO experiments on Japanese medaka ( Dasmahapatra et al, 2022a , b ). In these experiments, we have used only one concentration of GO (20 mg/L) for exposure (96 h in GO with refreshing of media every 24 h and then depurated for 21 days in a GO-free environment).…”

“…The GO exposure was continued for 96 h with refreshing of media every 24 h. During exposure (4 days) and post-exposure periods (21 days) the fish were fed twice (as per a.m. and p.m. schedule with brine shrimp nauplii and Tetramin Flakes) and the collection of eggs from each experimental tank were continued. After required period of treatment (96 h), the fish were transferred to fresh BSS with no GO and maintained in clean 1 L glass jars for 21 days with refreshing of media every 24 h and continued egg collection until the end of the experiment ( Dasmahapatra and Tchounwou, 2022a ; b ). After 21days depuration (post-exposure), the survived fish (all nine pairs; no death) were anesthetized in MS 222 (100 mg/L) and processed for immunohistochemistry ( OECD, 2009 ).…”

“…For the past several years, significant efforts have been made by OECD, USEPA, EU, and others, to develop strategies for screening and identifying EDCs using model organisms living in different ecosystems ( OECD, 2018 ). Our laboratory has been evaluating GO, as a potential EDC using Japanese medaka fish ( Oryzias latipes ) as the experimental model ( Dasmahapatra et al, 2020a ; b ; Myla et al, 2021a ; b ; Asala et al, 2021 , 2022 ; Dasmahapatra and Tchounwou, 2022a ; b ). Our studies demonstrated that the medaka males are more sensitive to GO than the females ( Dasmahapatra et al, 2020a ; b ).…”