Experimental design optimization of the capillary electrophoresis separation of leucine enkephalin and its immune complex

Babar, Sheikh Md. Enayetul; Song, Eun Joo; Hasan, Md Nabiul; Yoo, Young Sook

doi:10.1002/jssc.200700145

Cited by 11 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the Box-Behnken method has been applied to several affinity CE methods, for example, by predicting dissociation constants of carbonic anhydrase and its ligand as a function of injection time, capillary length, and applied voltage [24]. Also, buffer pH, concentration, and applied voltage were varied to optimize peak efficiencies, migration time, and resolution of leucine enkephalin and its immuno complex [25]. …”

Section: Introductionmentioning

confidence: 99%

Optimization of capillary electrophoresis conditions for a glucagon competitive immunoassay using response surface methodology

et al. 2009

View full text Add to dashboard Cite

The capillary electrophoresis (CE) conditions for a competitive immunoassay of glucagon were optimized for highest sensitivity of the immunoassay and resolution of the electrophoretic peaks using a Box-Behnken design. Injection time, voltage ramp time, and separation voltage were varied between three levels and two responses, bound-to-free (B/F) ratio of the immunoassay peaks and resolution between the peaks, were measured. Analysis of variance was applied to fit a predictive model, and a desirability function was used to simultaneously optimize both responses. A 10 sec injection, 1.6 min ramp time, and a 22 kV separation voltage were the conditions found when high B/F was given more emphasis than high resolution. To test the model, calibration curves of a glucagon immunoassay were measured at the optimum and least optimum CE conditions. Optimal conditions increased the sensitivity of the immunoassay by 388% compared to the least optimum conditions while maintaining adequate resolution.

show abstract

Section: Introductionmentioning

confidence: 99%

Optimization of capillary electrophoresis conditions for a glucagon competitive immunoassay using response surface methodology

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Previously it has been shown that the choice of buffer can affect immune complex formation . We tested separations in 1× TBE, pH 8.0 with 1% w/v MC‐88 000.…”

Section: Resultsmentioning

confidence: 99%

Microchip immunoaffinity electrophoresis of antibody–thymidine kinase 1 complex

2015

View full text Add to dashboard Cite

Thymidine kinase-1 (TK1) is an important cancer biomarker whose serum levels are elevated in early cancer development. We developed a microchip electrophoresis immunoaffinity assay to measure recombinant purified TK1 (pTK1) using an antibody that binds to human TK1. We fabricated poly(methyl methacrylate) microfluidic devices to test the feasibility of detecting antibody (Ab)-pTK1 immune complexes as a step towards TK1 analysis in clinical serum samples. We were able to separate immune complexes from unbound antibodies using 0.5X phosphate buffer saline (pH 7.4) containing 0.01% Tween-20, with 1% w/v methylcellulose that acts as a dynamic surface coating and sieving matrix. Separation of the antibody and Ab-pTK1 complex was observed within a 5 mm effective separation length. This method of detecting pTK1 is easy to perform, requires only a 10 μL sample volume, and takes just 1 minute for separation.

show abstract

“…For the optimization of chemical parameters of biodiesel production process from palm oil we used the most efficient method found in our previous work [12]. The Box-Behnken design was employed as a statistical means to optimize this production process [13].…”

Section: Resultsmentioning

confidence: 99%

Transesterification of Palm Oil to Biodiesel and Optimization of Production Conditions i.e. Methanol, Sodium Hydroxide and Temperature

Siddiqua¹

2015

JENR

View full text Add to dashboard Cite

Biodiesel is an alkyl ester of long chain fatty acids and considered as an alternative to lower the appalling consequence of fuel on the environment. It is produced by transesterification of a fat or oil with a short chain primary alcohol like methanol and alkali like sodium hydroxide (NaOH). Palm oil (Elaeis guineensis) was used as source to produce biodiesel and Box Behnken experimental design was applied to see the effect of various process parameters, i.e. methanol quantity, alkali concentration and temperature for the optimization of calorific value of biodiesel. Response surface plots and contour plot were created in order to perceive the optimum condition. Though, all the three variables significantly affected the calorific value of the palm biodiesel, but it was found that methanol was more effective variable than alkali concentration and temperature. It was observed that 12.5 ml methanol/50 ml oil and 0.4 gm NaOH/50 ml oil and 55°C temperature were optimum condition, where the calorific value of palm biodiesel is 9297.206 kcal/kg.

show abstract

Experimental design optimization of the capillary electrophoresis separation of leucine enkephalin and its immune complex

Cited by 11 publications

References 18 publications

Optimization of capillary electrophoresis conditions for a glucagon competitive immunoassay using response surface methodology

Optimization of capillary electrophoresis conditions for a glucagon competitive immunoassay using response surface methodology

Microchip immunoaffinity electrophoresis of antibody–thymidine kinase 1 complex

Transesterification of Palm Oil to Biodiesel and Optimization of Production Conditions i.e. Methanol, Sodium Hydroxide and Temperature

Contact Info

Product

Resources

About