Experimental Diabetes Model in Chick Embryos Treated with Streptozotocin

Abstract

The aim of the present study was to investigate whether diabetes model can be made by treatment of streptozotocin (STZ) in chick embryos and this model can be used to predict the effect of drug. When STZ (0.3 mg/egg) was injected into the albumen of fertile eggs on the 14th day of incubation, level of blood glucose significantly increased than that of the control on the 17th day of incubation, and level of serum insulin significantly decreased. In addition, the enhanced level of blood glucose in STZ-treated embryos reduced by injection of human insulin. In conclusion, STZ-treated embryos may be applicable to evaluate human insulin and anti-diabetes drugs as an experimental diabetes model.

The chick eye in vision research: An excellent model for the study of ocular disease

“…In contrast to the rat, adult chicks have been reported to be resistant to streptozotocin-induction of diabetes (Simon and Dubois, 1980; Stellenwerf and Hazelwood, 1979). However, chick embryos appear amenable to streptozotocin diabetes induction (Shi et al, 2014; Yoshiyama et al, 2005). Yoshiyama et al (2005) injected streptozotocin into the albumen of fertilized eggs day 14 of incubation and showed decreased insulin and elevated glucose levels at day 17.…”

“…However, chick embryos appear amenable to streptozotocin diabetes induction (Shi et al, 2014; Yoshiyama et al, 2005). Yoshiyama et al (2005) injected streptozotocin into the albumen of fertilized eggs day 14 of incubation and showed decreased insulin and elevated glucose levels at day 17. Shi et al (2014) then demonstrated that injection of streptozotocin in the amniotic layer at embryonic day 12 produced typical ocular sequelae of diabetes, including cataract, retinal thinning, ERG abnormalities, and insulin-related signaling changes that were similar to those reported in the streptozotocin rat model.…”

Modeling Chick to Assess Diabetes Pathogenesis and Treatment

“…Malformations associated with diabetic pregnancy affect many major organ systems, such as the central nervous, cardiovascular, gastrointestinal, urogenital, and musculoskeletal systems [83]. The infant of the diabetic mother has an increased risk for several neonatal complications, such as macrosomia, hypoglycemia, hypocalcemia, polycythemia, and hyperbilirubinemia.…”

Hambatan EGCG terhadap Ekspresi Vascular Endothelial Growth Factor (VEGF) dan Ekspresi Vascular -endothelial Cadherin (VE-Cadherin) dalam Vaskulo-angiogenesis Embrio ayam

“…Angiogenesis diperlukan dalam proses pertumbuhan embrio, maupun proses-proses perbaikan fisiologis saat postnatal. Proses vaskulo-angiogenesis pada embrio sering dipelajari dengan menggunakan embrio ayam (maupun famili aves lainnya) karena lebih mudah dipelajari [3,8], serta untuk mengurangi penggunaan mamalia sebagai hewan coba [9]. Angiogenesis yang berjalan abnormal mengakibatkan berbagai proses patologis.…”