Experimental estimation of postmortem interval using multivariate analysis of proton NMR metabolomic data

Hirakawa, Keiko; Koike, Kazuhiko; Uekusa, Kyoko; Nihira, Makoto; Yuta, Kohtaro; Ohno, Youkichi

doi:10.1016/j.legalmed.2009.02.007

Cited by 26 publications

(18 citation statements)

References 22 publications

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“…The analysis of the metabolome modifications, relying on multiple changes of the global profile, seems to be more promising than traditional methods for a reliable PMI estimation. Several works have been devoted to the use of NMR metabolomics of BFs or tissues to monitor the modifications in endogenous metabolites induced by death and to find potential PMI biomarkers (Hirakawa et al 2009 ; Rosa et al 2015 ; Zelentsova et al 2016 ; Snytnikova et al 2017a , 2017b ). Different animal models have been studied and several biological matrices, such as blood, muscles, VH, AH, and cornea have been analysed.…”

Section: Metabolome Modifications After Deathmentioning

confidence: 99%

Forensic NMR metabolomics: one more arrow in the quiver

et al. 2020

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Introduction NMR metabolomics is increasingly used in forensics, due to the possibility of investigating both endogenous metabolic profiles and exogenous molecules that may help to describe metabolic patterns and their modifications associated to specific conditions of forensic interest. Objectives The aim of this work was to review the recent literature and depict the information provided by NMR metabolomics. Attention has been devoted to the identification of peculiar metabolic signatures and specific ante-mortem and post-mortem profiles or biomarkers related to different conditions of forensic concern, such as the identification of biological traces, the estimation of the time since death, and the exposure to drugs of abuse. Results and Conclusion The results of the described studies highlight how forensics can benefit from NMR metabolomics by gaining additional information that may help to shed light in several forensic issues that still deserve to be further elucidated.

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Section: Metabolome Modifications After Deathmentioning

confidence: 99%

Forensic NMR metabolomics: one more arrow in the quiver

et al. 2020

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“…The use of metabolomics in PMI is not limited to the microbiome. In 2009, Hirakawa et al [21] investigated metabolic alterations after death in rat muscles by proton ( 1 H) NMR spectra. The results of the study suggested that the metabolic profiles of the tissues varied in accordance with the manner of death, and correlated with PMI, as well.…”

Section: Role Of Metabolomics In Forensic Medicine 2121 Postmortementioning

confidence: 99%

“…NMR-based metabolic profiling Hirakawa et al [21] MS-based metabolic profiling Brockbals et al [14] Dai et al [22] Wu et al [23] Du et al [24] Kaszynski et al [25] Sato et al [26] Kang et al [27] Intoxication and abuse of drugs MS-based metabolic profiling Kim et al [7] Shima et al [29] Tsai et al [5] Krumbiegel et al [30] Diagnosis of disease and cause of death NMR-based metabolic profiling Ghauri et al [38] MS-based metabolic profiling Hu et al [37] Proteomics Postmortem interval…”

Section: Postmortem Intervalmentioning

confidence: 99%

Omics era in forensic medicine: towards a new age

Akçan¹,

Taştekin²,

Yıldırım³

et al. 2020

Turk J Med Sci

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Introduction The topic "forensic medicine and sciences" is a subdivision of science which is described as the practice of medical/ paramedical scientific knowledge to establish facts in civil and criminal law [1]. Forensic medicine has a multidisciplinary structure in collaboration with other areas such as forensic chemistry, toxicology, biology, psychiatry, and genetics, etc. Since forensic sciences share a common scientific methodology with other science disciplines it is open to collaborate or benefit from novel technologies appeared in any scientific discipline. Therefore, omics emerges as a revolutionary step in forensic sciences and sure carries it towards a new age. The publications regarding omics in various disciplines have become a rising scientific trend worldwide, during the last decade. Furthermore, the number of forensic studies utilizing the methodology of omics has enormously increased in last years. In order to draw attention of forensic and medical professionals, and encourage new studies; the roles and benefits of omics in forensic medicine and sciences are discussed in this review. 2. Omics Omics is a novel multidisciplinary field of various scientific methodologies. Omics defines the collective technologies that measure some characteristics (roles, relationships, and actions) of certain molecules like metabolites, proteins and genes, ending in-omics, as in metabolomics, proteomics, genomics, etc. In past years, omics technologies were applied to diverse fields, because of the ability to assess little changes in a large-scale data with exhaustive metabolite evaluation [2]. With the development of technology, omics-based strategies become cheaper, faster, and very informative, and offer potent alternatives to conventional technics. Furthermore, sample selection has a key role to success in target study. Various human samples can be used for analysis such as blood [3,4], urine [5], hair [6,7], nail [8-10], faeces[11,12], aqueous humour [13] etc. The interactions of omics with each other have been shown in Figure. 2.1. Metabolomics 2.1.1. Definition of metabolomics Metabolomics comprises the studies that are focused on assessment of metabolites and related markers of an Background/aim: Forensic medicine and sciences is a multidisciplinary branch of science, which frequently benefit from novel technologies. State of the art omics technologies have begun to be performed in forensic medicine and sciences, particularly in postmortem interval, intoxication, drugs of abuse, diagnosis of diseases and cause of death. This review aims to discuss the role and use of great omics (metabolomics, proteomics, genomics and transcriptomics) in forensic sciences, in detail. Materials and methods: A detailed review of related literature was performed, and studies were subdivided as per the type of omics. Results and conclusion: Omics seems as a revolutionary step in forensic science and sure carries it towards a new age. The number of forensic studies utilizing omics steadily increases in last years. Omics strategi...

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“…Metabolic profiling utilizes high-resolution analytical methods, such as nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS), for the quantitative analysis of hundreds of small molecules (<1000 Da) in biological samples [ 6 ]. Several studies have been working on the postmortem metabolic characteristics by taking advantages of 1 hydrogen-nuclear magnetic resonance ( 1 H-NMR) [ 7 – 9 ]. Nonetheless, merely components with high abundance in tissues could be identified by means of 1 H-NMR due to its limited dynamic range and low sensitivity [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling of femoral muscle from rats at different periods of time after death

Lin

Xie

et al. 2018

PLoS ONE

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Clarification of postmortem metabolite changes can help characterize the process of biological degradation and facilitate investigations of forensic casework, especially in the estimation of postmortem interval (PMI). Metabolomics can provide information on the molecular profiles of tissues, which can aid in investigating postmortem metabolite changes. In this study, liquid chromatography-mass spectrometric (LC-MS) analysis was performed to identify the metabolic profiles of rat femoral muscle at ten periods of time after death within 168 h. The results obtained by reversed-phase liquid chromatography (RPLC)- and hydrophilic interaction liquid chromatography (HILIC)- electrospray ionization (ESI±) have revealed more than 16,000 features from all four datasets. Furthermore, 915 of these features were identified using an in-house database. Principal component analysis (PCA) demonstrated the time-specific features of molecular profiling at each period of time after death. Moreover, results from partial least squares projection to latent structures-discriminant analysis (PLS-DA) disclosed a strong association of metabolic alterations of at least 59 metabolites with the time since death, especially within 48 h after death, which expounds these metabolites as potential indicators in PMI estimation. Altogether, our results illustrate the potentiality of metabolic profiling in the evaluation of PMI and provide candidate metabolite markers with strong correlation with time since death for forensic purpose.

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Experimental estimation of postmortem interval using multivariate analysis of proton NMR metabolomic data

Cited by 26 publications

References 22 publications

Forensic NMR metabolomics: one more arrow in the quiver

Forensic NMR metabolomics: one more arrow in the quiver

Omics era in forensic medicine: towards a new age

Metabolic profiling of femoral muscle from rats at different periods of time after death

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