Experimental etch-and-rinse adhesives doped with bioactive calcium silicate-based micro-fillers to generate therapeutic resin–dentin interfaces

Profeta, Andrea Corrado; Mannocci, Francesco; Foxton, Richard M.; Watson, Timothy F.; Feitosa, Victor Pinheiro; Carlo, B. De; Mongiorgi, R; Valdrè, Giovanni; Sauro, Salvatore

doi:10.1016/j.dental.2013.04.001

Cited by 66 publications

(66 citation statements)

References 53 publications

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“…This hypothesis was also validated when immersing the specimens in the xylenol orange solution (Profeta et al, 2013;Toledano et al, 2013). In general, load cycled specimens treated with XO-dye produced a clearly outlined fluorescence due to a consistent Ca-minerals deposited within the bonding interface and inside the dentinal When specimens were load cycled in held 24 h, the complex moduli achieved at peritubular and intertubular dentine attained the highest values (68.45 and 80.62 GPa, respectively) among all tests, and it was ~2.74 and 4.72 fold higher than values attained in the unloaded groups (25 and 17 GPa, respectively).…”

Section: Resultsmentioning

confidence: 90%

“…When sustained load was applied for 72 h, a tight fluorescein sorption throughout the thickness of the HL was detected, which extended until the total adhesive thickness, but it was more concentrated in the dentine vicinity (Figs 3/5A, 3/5B). In some tubules, a discrete spectral overlap (yellow) was appreciated in the emission of profile of both dyes (red and green), indicating further pathways of water uptake, presence of micro-canals for the advance of permeability and future degradation of the dentine-polymer interaction through nanoleakage (Profeta et al, 2013;Toledano et al, 2013). This mineral depleted and non-sufficiently infiltrated etched dentine was also observable as a dentine substrate scarcely remineralised, at both intertubular and peritubular locations (Fig 2E), indicating the pathway for further degradation.…”

Section: Resultsmentioning

confidence: 99%

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Nanoscopic dynamic mechanical analysis of resin–infiltrated dentine, under in vitro chewing and bruxism events

Toledano

Osorio

Cabello

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

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HIGHLIGHTSSustained mechanical loading promotes failures at the resin-dentine interface.Higher viscoelastic properties of tubular dentine concentrate stress at the interface.Load cycling in sine or square wave promote healing at the resin-dentine interface. 4 ABSTRACTThe aim of this study was to evaluate the induced changes in mechanical behavior and bonding capability of resin-infiltrated dentine interfaces, after application of mechanical stimuli. Dentine surfaces were subjected to partial demineralisation through 37% phosphoric acid etching followed by the application of an etch-and-rinse dentine adhesive, Single Bond (3M/ESPE). Bonded interfaces were stored in simulated body fluid during 24 h, and then tested or submitted to the mechanical loading challenge.Different loading waveforms were applied: No cycling (I), 24 h cycled in sine (II) or square (III) waves, sustained loading held for 24 h (IV) or sustained loading held for 72 h (V). Microtensile bond strength (MTBS) was assessed for the different groups.Debonded dentine surfaces were studied by field emission scanning electron microscopy (FESEM). At the resin-dentine interface, both the hybrid layer (HL) and the bottom of the hybrid layer (BHL), and both peritubular and intertubular were evaluated using a nanoindenter in scanning mode. The load and displacement responses were used to perform the nano-Dynamic Mechanical analysis and to estimate the complex and storage modulus. Dye assisted Confocal Microscopy Evaluation was used to assess sealing ability. Load cycling increased the percentage of adhesive failures in all groups.Specimens load cycled in held 24 h attained the highest complex and storage moduli at HL and BHL. The storage modulus was maximum in specimens load cycled in held 24 h at peritubular dentine, and the lowest values were attained at intertubular dentine. The storage modulus increased in all mechanical tests, at peritubular dentine. An absence of micropermeability and nanoleakage after loading in sine and square waveforms were encountered. Porosity of the resin-dentine interface was observed when specimens were load cycled in held 72 h. Areas of combined sealing and permeability were discovered 5 at the interface of specimens load cycled in held 24h. Crack-bridging images appeared in samples load cycled with sine waveform, after FESEM examination.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Nanoscopic dynamic mechanical analysis of resin–infiltrated dentine, under in vitro chewing and bruxism events

Toledano

Osorio

Cabello

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

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“…The rest of the molars were immersed in 0.5 wt% xylenol orange solution (Xo: Sigma-Aldrich Chemie Gmbh, Riedstr, Germany), excited at 514-nm for 24 h at 37 ºC (pH 7.2). The latter is a calcium-chelator fluorophore commonly used in hard tissues remineralization studies, due to its ability to form complexes with divalent calcium ions (Profeta et al, 2013). Specimens were copiously rinsed with water and treated in an ultrasonic water bath for 2 min.…”

Section: Confocal Microscopy Evaluationmentioning

confidence: 99%

“…The resolution of CLSM images was 1024 x 1024 pixels. Five optical images were randomly captured from each resin-dentin interface, and micrographs representing the most common features of nanoleakage observed along the bonded interfaces were selected (Profeta et al, 2013;Toledano et al, 2013b). Three specimens from each subgroup were immersed.…”

Section: Confocal Microscopy Evaluationmentioning

confidence: 99%

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A zinc chloride-doped adhesive facilitates sealing at the dentin interface: A confocal laser microscopy study

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

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The aim of this study was to understand the effect of Zn-doping of adhesives and mechanical load cycling on the micromorphology of the resin-dentin interdiffusion zone (of sound and caries affected dentin). The investigation considered two different Zndoped adhesive approaches and evaluated the interface using confocal laser scanning microscopy. Sound and carious dentin-resin interfaces of unloaded specimens were deficiently resin-hybridized, in general. These samples showed a rhodamine B-labeled hybrid layer and adhesive layer completely affected by fluorescein penetration (nanoleakage) through the porous resin-dentin interface, but thicker after phosphoric acid-etching and more extended in carious dentin. Zn-doping promoted an improved sealing of the resin-dentin interface at dentin, a decrease of the hybrid layer porosity, and an increment of dentin mineralization. Load cycled augmented the sealing of the Zn-doped resin-dentin interfaces, as porosity and nanoleakage diminished, and even disappeared in caries-affected dentin substrata conditioned with EDTA. Sound and carious dentin specimens treated with the xylenol orange technique produced a clearly outlined fluorescence when resins were Zn-doped, due to a consistent Ca-mineral deposits within the bonding interface and inside the dentinal tubules, especially when load cycling was applied on specimens treated with Zn-doped bonding components of self-etching adhesives. Micropermeability at the resin-dentin interface diminished after combining EDTA pretreatment, ZnCl2-doping and mechanical loading stimuli on restorations. It is clearly preferable to include the zinc compounds into the bonding constituents of the self-etching adhesives, instead of into the primer ingredients. The promoted new mineral segments contributed to reduce or avoid both porosity and nanoleakage from the load cycled Zn-doped resin dentin interface.

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Evaluating the shear bond strength and remineralization effect of calcium silicate‐based and conventional self‐adhesive resin cements to caries‐affected dentin

Tavangar

Safarpour

Parizi

et al. 2022

Clinical & Exp Dental Res

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Objective Given the importance of preserving caries‐affected dentin (CAD) in conservative dentistry, the shear bond strength (SBS) of different resin cements to CAD has been investigated. Here, we aimed to compare the SBS and remineralizing effect of a calcium silicate (TheraCem) and conventional self‐adhesive cement (Panavia SA) on the SBS of CAD. Materials and Methods Forty‐eight extracted third molars (24 sound and 24 CAD) were used. In each group, 12 teeth were prepared for bonding to TheraCem or Panavia SA. After removal of the enamel and caries, resin composite cylinders were luted on the prepared dentin. After 28 days of storage in the artificial saliva, SBS was measured and the failure mode analysis was investigated. The images of fractured sections were analyzed using scanning electron microscopy and energy‐dispersive X‐ray to evaluate the Ca/P weight ratio. Results SBS of CAD and sound dentin was not different when cemented with TheraCem (9.56 ± 4.51 vs. 9.17 ± 2.76, p = .806), but the CAD showed significantly lower SBS to Panavia SA (9.4 ± 2.36 vs. 7.39 ± 2.18, p = .015). The Ca/P ratio in CAD was significantly higher when bonded to both TheraCem and Panavia‐SA than that of the controls (p = .001); however, this ratio was not different for those bonded to TheraCem compared to Panavia SA. Conclusions Based on our results, TheraCem as a calcium silicate cement shows better SBS to attach the restoration to CAD as compared to Panavia SA. Obliteration and mineralization of the dentinal tubules in TheraCem were also higher than in Panavia SA. However, their ability to improve the amount of the Ca/P ratio in CAD was similar.

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Experimental etch-and-rinse adhesives doped with bioactive calcium silicate-based micro-fillers to generate therapeutic resin–dentin interfaces

Cited by 66 publications

References 53 publications

Nanoscopic dynamic mechanical analysis of resin–infiltrated dentine, under in vitro chewing and bruxism events

Nanoscopic dynamic mechanical analysis of resin–infiltrated dentine, under in vitro chewing and bruxism events

A zinc chloride-doped adhesive facilitates sealing at the dentin interface: A confocal laser microscopy study

Evaluating the shear bond strength and remineralization effect of calcium silicate‐based and conventional self‐adhesive resin cements to caries‐affected dentin

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