Experimental evaluation of the efficacy of sanitation procedures in operating rooms

Frabetti, Alessia; Vandini, Alberta; Balboni, Pier Giorgio; Triolo, Fabio; Mazzacane, Sante

doi:10.1016/j.ajic.2009.03.011

Cited by 22 publications

(21 citation statements)

References 10 publications

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“…[5] Colony counting is a verified method for determining contamination rate. [6] To measure the sensitivity of our tests, swabs outside the surgical field were taken and cultured for growth. Pre- and postoperative colony counts and postoperative counts from MIS and traditional discectomies were compared using the paired Student t -test.…”

Section: Methodsmentioning

confidence: 99%

Comparison of operating field sterility in open versus minimally invasive microdiscectomies of the lumbar spine

Yew²,

Kimball³

et al. 2013

Surg Neurol Int

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Background:Postoperative wound infection is a preventable risk that can lead to significant adverse outcomes and increased cost of care. Minimally invasive surgeries (MIS) have been found to have lower rates of postoperative infection compared with the traditional approach. To assess if the reported difference is related to intraoperative contamination or to other factors, we assessed the surgical field for sterility.Methods:We compared 10 MIS versus 10 traditional microdiscectomies. Swabs of the operating field were obtained before and after the procedure from multiple sites in the operating room. Positive and negative controls were taken of the skin immediately before and after preparation of the incision site. All swabs were plated out on Columbia blood agar plates and grown for 48 hours. Colony counting was performed to determine growth.Results:There was no statistically significant difference in the colony counts of swab sites in traditional microdiscectomies compared with MIS microdiscectomies. There was no significant contamination of the operating field using either approach.Conclusions:In this prospective study, we found that there was no significant difference in bacterial counts in swabs of operative sites in either traditional or MIS microdiscectomies, suggesting that the decreased rate of postoperative infection in the reported literature for MIS cases may be related to other factors, such as patient selection and/or postoperative care.

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Section: Methodsmentioning

confidence: 99%

Comparison of operating field sterility in open versus minimally invasive microdiscectomies of the lumbar spine

Yew²,

Kimball³

et al. 2013

Surg Neurol Int

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“…Ultrasound examinations using the probes and sterile gel were then simulated on cowhide pieces (20 9 20 cm) that had been sterilized with ethylene oxide gas. We measured the number of bacteria transmitted from the volunteers to the cowhide by pressing Rodac plates (Becton-Dickinson, Sparks, MD, USA) containing 5% sheep blood agar [6] onto the cowhide surface and then incubating the plates at 37°C for 4 days. Thereafter, bacterial colonies on the plates were counted, and the number of bacteria (CFU) transmitted by ultrasound examination was determined.…”

Section: Bacterial Transmission Via Ultrasound Probesmentioning

confidence: 99%

Evaluation of procedures for decontaminating ultrasound probes

et al. 2011

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“…It is clear that microorganisms can adapt to a variety of environmental physical and chemical conditions, and it is therefore not surprising that resistance to extensively used antiseptics and disinfectants has been reported [20], [21]. For these reasons, the importance of cleaning procedures that are aimed to control the load of pathogenic bacteria indicates that a new and sustainable strategy is necessary.…”

Section: Introductionmentioning

confidence: 99%

Hard Surface Biocontrol in Hospitals Using Microbial-Based Cleaning Products

et al. 2014

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BackgroundHealthcare-Associated Infections (HAIs) are one of the most frequent complications occurring in healthcare facilities. Contaminated environmental surfaces provide an important potential source for transmission of many healthcare-associated pathogens, thus indicating the need for new and sustainable strategies.AimThis study aims to evaluate the effect of a novel cleaning procedure based on the mechanism of biocontrol, on the presence and survival of several microorganisms responsible for HAIs (i.e. coliforms, Staphyloccus aureus, Clostridium difficile, and Candida albicans) on hard surfaces in a hospital setting.MethodsThe effect of microbial cleaning, containing spores of food grade Bacillus subtilis, Bacillus pumilus and Bacillus megaterium, in comparison with conventional cleaning protocols, was evaluated for 24 weeks in three independent hospitals (one in Belgium and two in Italy) and approximately 20000 microbial surface samples were collected.ResultsMicrobial cleaning, as part of the daily cleaning protocol, resulted in a reduction of HAI-related pathogens by 50 to 89%. This effect was achieved after 3–4 weeks and the reduction in the pathogen load was stable over time. Moreover, by using microbial or conventional cleaning alternatively, we found that this effect was directly related to the new procedure, as indicated by the raise in CFU/m2 when microbial cleaning was replaced by the conventional procedure. Although many questions remain regarding the actual mechanisms involved, this study demonstrates that microbial cleaning is a more effective and sustainable alternative to chemical cleaning and non-specific disinfection in healthcare facilities.ConclusionsThis study indicates microbial cleaning as an effective strategy in continuously lowering the number of HAI-related microorganisms on surfaces. The first indications on the actual level of HAIs in the trial hospitals monitored on a continuous basis are very promising, and may pave the way for a novel and cost-effective strategy to counteract or (bio)control healthcare-associated pathogens.

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Experimental evaluation of the efficacy of sanitation procedures in operating rooms

Cited by 22 publications

References 10 publications

Comparison of operating field sterility in open versus minimally invasive microdiscectomies of the lumbar spine

Comparison of operating field sterility in open versus minimally invasive microdiscectomies of the lumbar spine

Evaluation of procedures for decontaminating ultrasound probes

Hard Surface Biocontrol in Hospitals Using Microbial-Based Cleaning Products

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