Experimental Evaluation of the Impact of Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles on the Carrageenan-Induced Intestinal Inflammation

Abstract

Aim: To evaluate the effects of orally administered gadolinium orthovanadate GdVO4:Eu3+ nanoparticles (VNPs) on the course of chronic carrageenan-induced intestinal inflammation. Methods: Samples of small intestinal tissue were collected from four groups of rats (intact, after administration of VNPs, with carrageenaninduced intestinal inflammation, with carrageenan-induced intestinal inflammation orally exposed to VNPs) to assess the intestinal morphology and HSP90α expression. Levels of seromucoid, C-reactive protein, TNF-α, IL-1β and IL-10 were determined in blood serum. Results: Oral exposure to VNPs was associated with neither elevation of inflammation markers in blood serum nor HSP90α overexpression in the small intestine, i.e. no toxic effects of VNPs were observed. Carrageenan-induced intestinal inflammation was accompanied by higher levels of TNF-α and IL-1β, as well as HSP90α upregulation in the intestinal mucosa, compared with controls. Administration of VNPs to rats with enteritis did not lead to statistically significant changes in concentrations of circulating pro-inflammatory cytokines with the trend towards their increase. Conclusion: No adverse effects were observed in rats orally exposed to VNPs at a dose of 20 μg/kg during two weeks. Using the experimental model of carrageenan-induced enteritis, it was demonstrated that VNPs at the dose used in our study did not affect the course of intestinal inflammation.

Effect of the food additive E407a on E-cadherin expression in the small intestine

“…Балл от 0 до 5 присваивался при наличии, соответственно, 0-2, 3-5, 6-8, 9-11, 12-14, 15 и более окрашенных клеток. В каждом микропрепарате оценивали 5 полей зрения [11].…”

Advances in the Study of Marine Products with Lipid-Lowering Properties

“…Some authors demonstrated that the low molecular weight carrageenan significantly reduces blood serum levels of TC, TG and LDL-C and improves the concentration of HDL-C in HFD fed mice, with a much stronger activity than that of high molecular weight carrageenan [50]. Furthermore, a considerable number of studies have shown that carrageenan causes inflammation [51][52][53], which should be fully considered in further studies, as this puts forward some limitations on the risk-free application of carrageenan. Although with certain side effects, further in-depth research on molecular mechanisms will help the development and utilization of Carrageenan.…”