Experimental Evidence for the Influence of Cumulus-Oocyte-Complexes on Sperm Release from the Porcine Oviductal Sperm Reservoir

Brüssow, Klaus‐Peter; Torner, H.; Rátky, J.; Manabe, Noboru; Tuchscherer, Armin

doi:10.1262/jrd.17085

Cited by 21 publications

(24 citation statements)

References 49 publications

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“…Accordingly, the present article has not discussed the influence of 'the products of ovulation' entering the lumen of the oviduct (Harper, 1973a,b), although there have been repeated reviews of the topic (e.g., Hansen et al, 1991;Hunter, 1995Hunter, , 1998Brü ssow et al, 1999Brü ssow et al, , 2006. There is, moreover, the important caveat that no evidence has yet been published to indicate passage of whole follicular fluid along the length of the oviduct and into the isthmus reservoir soon after the time of ovulation.…”

Section: Influence Of Follicular Fluidmentioning

confidence: 99%

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Hunter

2007

Molecular Reproduction Devel

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To avoid inappropriate conclusions being drawn from the extensive use of in vitro preparations of sperm-oviduct epithelial binding, it is recalled that events in the genital tract of mammals are regulated by the gonads, primarily by their changing secretion of steroid hormones. Key observations from in vivo models are used to emphasise the dynamic interactions between viable sperm cells and the caudal (distal) portion of the oviduct isthmus, the site of the functional sperm reservoir. These include (1) preovulatory arrest and epithelial binding of intact sperm cells and thereby suppression of completion of capacitation, (2) peri-ovulatory activation and release from binding of discrete sub-populations of competent spermatozoa, and (3) post-ovulatory liberation of large numbers of spermatozoa. These observations underline the influence of endocrine regulation of sperm binding and release by peri-ovulatory Graafian follicles, a point brought out by the enhanced sperm release prompted by diverse treatments with solutions of progesterone. In the light of this evidence, the suitability of in vitro preparations for clarifying physiological events should be questioned, especially if myosalpingeal catecholamines diffusing out of the autonomic nervous system contribute to sperm activation and/or release. None of this is to infer that sperm cells themselves are without influence on their epithelial binding reaction(s). Nor is it to suggest that in vitro models of sperm-oviduct binding are without relevance to the development of sperm evaluation technologies. However, pre-ovulatory sperm-epithelial binding and a regulated peri-ovulatory release should be seen as vital tactics in the overall strategy of achieving successful monospermic fertilisation. Mol. Reprod. Dev. 75: 167-174, 2008.

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Section: Influence Of Follicular Fluidmentioning

confidence: 99%

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Hunter

2007

Molecular Reproduction Devel

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“…Laparoscopic intrauterine insemination is successfully used in sheep for different purposes (Gourley and Riese 1990;Moses et al 1997;Ehling et al 2003;PereyraBonnet et al 2011). In pigs such a method has been used only to a limited extent so far (Fantinati et al 2005;Brüssow et al 2006Brüssow et al , 2011. Although laparoscopic intrauterine insemination requires specialised technical equipment and expertise, it is fast and minimal invasive.…”

Section: Discussionmentioning

confidence: 99%

“…Laparoscopic intrauterine insemination was performed 31 h after hCG treatment with 20 ml/uterine horn of extended, fresh boar semen (32.2 × 106 sperm cells/ml; 65% motility). LIUI was carried out as described previously (Brüssow et al 2006(Brüssow et al , 2011. Briefly, general anaesthesia in gilts was induced with ketamine (0.15 ml/kg BW, Ursotamin ® , Serumwerk Dessau, Germany) and azaperone (0.03 ml/kg BW, Stresnil ® , Elanco Animal Health, Bad Homburg, Germany) and animals were fixed in a dorsal position.…”

Section: Methodsmentioning

confidence: 99%

“…Laparoscopic (oviductal) insemination was first described by Morcrom and Dukelow (1980). The potential of laparoscopic intrauterine insemination (LIUI) has been demonstrated in sperm-mediated gene transfer (Fantinati et al 2005) and evaluation of sperm migration (Brüssow et al 2006(Brüssow et al , 2011. The aim of the present study was to analyse the fertilization efficiency and the developmental diversity of embryos after single fixed-time LIUI.…”

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confidence: 99%

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Single fixed-time laparoscopic intrauterine insemination as a tool to obtain low-diversity porcine embryos

et al. 2013

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Double fixed-time insemination after ovulation induction is commonly used in pigs to obtain in vivo produced embryos at defined stages of development for downstream biotechnological applications. However, variations in the time of ovulation and fertilisation of the ovulated oocytes by spermatozoa, mainly in one of the inseminations, can cause diversities in embryo development. The aim of the present study was to reduce embryo diversity and to achieve a 'uniform outcome' of porcine embryo stages using single laparoscopic fixedtime insemination (LIUI). Altogether, 48 puberal German Landrace gilts were included in the study. Estrus of gilts was synchronized by 15-day long altrenogest (Regumate ® ) feeding and follicle development was stimulated with 850 IU eCG 24 h after the final altrenogest application. Ovulation was induced with 500 IU hCG 80 h after eCG. LIUI was performed 31 h after hCG treatment. Gilts under general anaesthesia were fixed in a dorsal position, a pneumoperitoneum was produced and three trocar cannulas were inserted into the abdomen for optics and instruments. Each uterine horn was carefully punctured 10-15 cm caudal from the utero-tubal junction with a 2.5 mm trocar. A 2.2 mm catheter was inserted about 3 cm into the uterine lumen and 20 ml of extended fresh boar semen (32.2 × 106 sperm cells/ml) were injected. Embryos were surgically flushed from the genital tract two (Day 2) and three (Day 3) days after insemination. Altogether, 778 oocytes/embryos were recovered (recovery rate 68 ± 17%); 45 of 48 gilts (93.8%) revealed fertilisation and 76.1% of the recovered embryos (n = 592) were at the 2-and 4-cell stage. On Day 2 (n = 22 gilts), a higher percentage of gilts (72.7%, P < 0.05) displayed only 2-cell embryos compared with gilts which had 2-and 4-cell (22.7%), or only 4-cell embryos (4.6%). On Day 3 (n = 23 gilts), the proportion of gilts with 2-cell, 2-and 4-cell, and only 4-cell embryos shifted to 4.3%, 0% and 95.7%, respectively (P < 0.05). The results of the present study demonstrate high rates of fertilisation and homogenously developed embryos after single fixed-time laparoscopic intrauterine insemination in gilts. Additionally, these results were achieved by inseminating a 60% lower number of sperm cells per insemination dose compared to usual doses used for transcervical insemination. In conclusion, LIUI can be recommended for the in vivo production of embryos in a homogeneous developmental stage, and also as an alternative method for low-dose insemination. Keywords: laparoscopic insemination; embryo diversity; pigList of abbreviations BW = body weight, CL = corpus luteum, eCG = equine chorionic gonadotropin, hCG = human chorionic gonadotropin, IU = international unit, LIUI = laparoscopic fixed-time inseminationIn vivo-derived embryos at a defined stage of development are often a necessary requirement for biotechnological applications and research. However, different developmental stages of the embryos (embryo diversity) at the time point of recovery remain an issue pigs (S...

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“…1): Group CONTROL (n=8) received IUI into the right horn, and the left horn served as nontreated control; Group LIGATURE (n=8) received IUI into the right horn, and the left horn was closed by endoscopic double ligature close to the bifurcation; and Group INTRAPERITONEAL (IPI, n=8) received IUI into the right uterine horn, the left horn was closed by double ligature and 20 ml of semen were deposited intraperitoneally at the surface of the left ovary. Endoscopic IUI was performed as described previously [19]. Briefly, anesthetized gilts were fixed in a dorsal position, and a pneumoperitoneum with CO2 was automatically produced (Endo Tech, Munich, Germany).…”

Section: Animals and Animal Treatmentmentioning

confidence: 99%

Sperm Migration in Pigs after Deep Intrauterine and Intraperitoneal Insemination

Brüssow

Torner

Rátky³

2011

Journal of Reproduction and Development

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Abstract. Deep intrauterine insemination in pigs allows sperm deposition only into one uterine horn, but bilateral fertilization of oocytes occurs. How the sperm reach the contralateral oviduct remains disputable. The aim of this experiment was to study possible transperitoneal and/or transuterine sperm migration ways. Follicle growth and ovulation were induced in 24 peripubertal gilts with eCG and hCG 72 h after eCG. Endoscopic intrauterine insemination (IUI) was performed 32 h after hCG with 20 ml of extended semen (60 × 10 6 spermatozoa) as follows: Group CONTROL (n=8) received IUI into the right horn, and the left horn served as non-treated control; Group LIGATURE (n=8) received IUI into the right horn, and the left horn was closed by endoscopic double ligature close to the bifurcation; Group INTRAPERITONEAL (IPI; n=8) received IUI into the right uterine horn, the left horn was closed by double ligature and semen was deposited intraperitoneally at the surface of the left ovary. Genital tracts were removed 65-66 h after hCG, the oviducts were flushed and ova (n=299) were analyzed for fertilization and cleavage. Furthermore, the accessory spermatozoa count/oocyte was graded as 0, without spermatozoa, 1, <5 spermatozoa, 2, 5-50 spermatozoa, 3, 50-100 spermatozoa and 4, >100 spermatozoa. The results indicate that low dose IUI into one horn provides a lower grade of accessory spermatozoa in the contra-lateral side (1.6 vs. 2.8). No spermatozoa were found in ova flushed from oviducts of the ligated uterine horn, even after intraperitoneal insemination (P<0.05), and no fertilization occurred, respectively. Our results clearly indicate that after low dose IUI into one uterine horn, spermatozoa reach the contralateral oviduct via transuterine migration.

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Experimental Evidence for the Influence of Cumulus-Oocyte-Complexes on Sperm Release from the Porcine Oviductal Sperm Reservoir

Cited by 21 publications

References 49 publications

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Single fixed-time laparoscopic intrauterine insemination as a tool to obtain low-diversity porcine embryos

Sperm Migration in Pigs after Deep Intrauterine and Intraperitoneal Insemination

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