Experimental immunology Exposure to hypergravity increases serum interleukin-5 and pulmonary infiltration in mice with allergic asthma

Abstract

ObjectiveWe evaluated the effect of acute hypergravity (HG) on the immune response in a murine model of allergic asthma.Material and methodsTwenty-eight BALB/c mice were used. Group A (control group, n = 7) mice were sensitized and challenged with normal saline. Group B (control HG exposure group, n = 7) mice were sensitized, challenged with saline, and exposed to acute HG (+10 Gz) for 4 hours. Group C (asthma group, n = 7) mice were challenged with intraperitoneal and intranasal ovalbumin (OVA) to induce asthma. Group D (asthma HG exposure group, n = 7) mice were exposed to HG for 4 hours after the induction of asthma. We estimated the total and OVA-specific serum IgE, serum titers of various cytokines, and the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid. Histopathology of the lung was also evaluated.ResultsThe serum level of interleukin (IL)-5 was significantly higher in Group D (12.9 ±4.9 pg/ml) compared to that in Group C (4.7 ±6.5 pg/ml, p = 0.017). In BAL fluid, the number of neutrophils was significantly increased in Group D compared to Group C (p = 0.014). Group D demonstrated a higher infiltration of inflammatory cells (9973.8 ±1642.7 cells/mm2) compared to Group C (7666.3 ±586.5 cells/mm2, p = 0.017). This tendency of increase in infiltration was not significant in non-asthmatic animals (Group A: 4488.8 ±176.1 cells/mm2 vs. Group B: 4946.3 ±513.7 cells/mm2, p > 0.05).ConclusionsAcute HG exacerbated the allergic response by increasing serum IL-5 levels and promoting pulmonary infiltration of inflammatory cells.

Hormetic Effect of Chronic Hypergravity in a Mouse Model of Allergic Asthma and Rhinitis

“…However, no studies have investigated the effect of chronic hypergravity in animals with allergic disorders. In our previous study, mice with allergic asthma showed no significant change in serum OVA-specific IgE after exposure to short-term hypergravity (10G for 4 hours) 20 . Therefore, we could suggest that changes in the allergic response in animals with allergic disorders may be unrelated to IgE-related mechanisms.…”

“…After exposure to short-term hypergravity, serum titers of IL-1β showed no significant change20. On the other hand, Liu and colleagues reported that IL-1β titers in rat brain were significantly increased after short-term exposure to hypergravity of 14G22.…”

Impacts of the Spaceflight to the Immune System

“…It might be the result of a stress response to rotatory stimuli. Another interesting aspect of this research is that, Jang et al [7] suggested that changes in the allergic response in animals with allergic disorders may be unrelated to IgE-related mechanisms, because of the study showed no significant change in serum OVA-specific IgE after exposure short term hypergravity (10 G for 4 hours).…”

Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes

“…A possible explanation for our results may be that the increased IL10 gene expression may represent a delicate regulatory pathway to restore the homeostasis under inflammatory conditions. Thus, IL10 may be a surrogate marker of a heightened inflammatory process and act to counterregulate the effects of proinflammatory mediators [ 35 , 40 ]. In regard to the correlation analysis, we demonstrated that IL10 gene expression was significantly positively correlated with lipid parameters and also with physical and glycemic parameters.…”