This work focused on the effects of laser energy density on the relative density, microstructure, and microhardness of Inconel 718 alloy manufactured by selective laser melting (SLM). The microstructural architectures, element segregation behavior in the interdendritic region and the evolution of laves phases of the as-SLMed IN718 samples were analyzed by optical metallography (OM), scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), and electron probe microanalysis (EPMA). The results show that with an increase in the laser volume energy density, the relative density and the microhardness firstly increased and then decreased slightly. It also facilitates the precipitation of Laves phase. The variation of mechanical properties of the alloy can be related to the densification degree, microstructure uniformity, and precipitation phase content of Inconel 718 alloy.