Experimental justification of the method of mesenchymal stem cell autotransplantation for regeneration of the femoral head bone tissue

Murzich, A.; Пашкевич, Л А; Жерносеченко, А. А.

doi:10.29235/1814-6023-2020-17-1-7-19

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…One of the current tasks in traumatology and orthopedics is the search for osteoplastic material with osteoconduction and osteoinduction effects on tissues exposed to a purulent process [1][2][3][4][5][6][7][8][9][10]. Today, cellular technologies are increasingly used in clinical situations associated with failed tissue repair.…”

Section: Introductionmentioning

confidence: 99%

Technology for Repairing Osteomyelitic Bone Defects Using Autologous Mesenchymal Stromal Cells on a Collagen Matrix in Experiment

Mitrofanov¹,

Живцов²,

Orlinskaya³

et al. 2021

Sovrem Tehnol Med

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The aim of the study was to develop a technology for repairing an osteomyelitic bone defect using autologous adipose tissue mesenchymal stromal cells (MSCs) bound to a collagen matrix and to test the efficacy of this technique. Materials and Methods. The study was carried out with 17 rabbits. A bone defect was created using a milling cutter applied to the proximal third of the leg. The wound (8.0×4.0 mm and a depth of 4.0 mm) involved the periosteum, cortical layer, and cancellous substance. Staphylococcus aureus strain was used as an infectious agent. After the development of chronic osteomyelitis, the animals underwent osteonecrectomy. In the study group, autologous MSCs in Collatamp EG collagen carrier were placed into the bone defect. MSCs were obtained from adipose tissue and cultured in the matrix for 5 days. In control, the defect was filled with the collagen matrix without cells. Results. On day 14 upon the initiation of chronic osteomyelitis, bacteriological examination of the discharge from the fistula showed the presence of mixed bacterial flora (Staphylococcus aureus and Escherichia coli) in all operated animals. Results of X-ray, laboratory, and histological tests confirmed the formation of a focus of chronic osteomyelitis. Two months after the treatment (collagen with or without MSCs) began, all animals of the study group showed mature bone tissue regenerated in the affected zone. In the control group, proliferation of osteoblasts on the surface of the bone trabeculae was also observed; however, mature osteoid tissue was more often detected in the study group (35.0 vs 20.0% in control). In the study group (MSCs + collagen matrix), there was a decrease in bone marrow fibrosis (50.0 vs 100.0% in control) and cartilage formation (30.0 and 66.7%, respectively). After full treatment, newly formed bone trabeculae were detected more often (100.0 vs 60.0% in control); they were more mature and filled the defect area more efficiently. Conclusion. Our results indicate that the use of a collagen matrix with autologous MSCs is a promising plastic material for repairing osteomyelitic defects following necrectomy. The MSCs were able to increase the density of the filling material in the bone cavity, significantly accelerate the formation of bone beams around the matrix, and increase the tissue volume around the implant. The presence of MSCs significantly decreased the interference of a connective tissue component with osteogenesis and chondrogenesis.

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Section: Introductionmentioning

confidence: 99%

Technology for Repairing Osteomyelitic Bone Defects Using Autologous Mesenchymal Stromal Cells on a Collagen Matrix in Experiment

Mitrofanov¹,

Живцов²,

Orlinskaya³

et al. 2021

Sovrem Tehnol Med

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Modern concepts of the osteogenic potential of mesenchymal stem cells and the creation of bioengineering structures for bone tissue repair

Жерносеченко

2020

Vescì Nac. akad. navuk Belarusì, Ser. med. navuk

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The following review summarizes the latest studies on in vitro osteogenic mesenchymal stem cell differentiation and selection of scaffolds that can maintain the viability and functional activity of these cells for bone tissue repair. In the last time, there have been investigated a lot of issues such as the stimulation and development osteogenic differentiation of MSCs, the growth factors – inducers of osteogenesis in MSCs, the creation of 3D constructions of cells in different scaffolds. A deeper understanding of the osteogenic differentiation mechanisms can result in the novel therapeutic opportunities of bone disease treatment. Special attention is given to materials for scaffold designs and template–cell interactions, which is of great importance for the structuring and functioning of an engineered tissue.

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Experimental justification of the method of mesenchymal stem cell autotransplantation for regeneration of the femoral head bone tissue

Cited by 2 publications

References 18 publications

Technology for Repairing Osteomyelitic Bone Defects Using Autologous Mesenchymal Stromal Cells on a Collagen Matrix in Experiment

Technology for Repairing Osteomyelitic Bone Defects Using Autologous Mesenchymal Stromal Cells on a Collagen Matrix in Experiment

Modern concepts of the osteogenic potential of mesenchymal stem cells and the creation of bioengineering structures for bone tissue repair

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