Experimental methods for investigating protein adsorption kinetics at surfaces

Abstract

The adsorption of proteins at the solid-liquid interface is a process of fundamental importance in nature. Extensive reviews (MacRitchie, 1978; Andrade & Hlady, 1986; Norde, 1986) testify to the strong interest which has been shown in the problem during the past few decades. Norde & Lyklema (1978) have rightly pointed out that protein adsorption is scientifically intriguing; the phenomenology is complicated and includes many presently apparently irreconcilable observations.

Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

“…The resonant peaks (from left to right, TM 0 and TE 0 ) are shifted to higher angles by the coating. The refractive index and the thickness of the guiding film were determined from the positions of the peaks before and after the coating procedure [1,48,58]. After coating the refractive index (n F ) decreased from 1.77494 to 1.77217 and the thickness (d F ) increased from 175.44 nm to 183.37 nm, similar to the result of the ellipsometric measurements.…”

“…Both instruments used a wavelength of 623.82 nm for the illuminating beam. 1 Each experiment was typically repeated three times. Images of surface-adhered cells were taken by an inverted phase contrast microscope (Zeiss Axiovert Observer) using 20× objectives.…”

“…The sensing of biomacromolecule concentration, affinity or adsorption using modern, label-free methods such as OWLS and QCM [1], requires that the objects to be sensed (e.g., proteins) are first captured by a sensor. Hence, it is advantageous for the sensor surface to be as large as possible because more analytes can be captured.…”

Applications of Nanoparticles in Biology

“…[21] A two-step binding process with a fast reversible association followed by a slower irreversible denaturation was established. [22] This interaction could be reversed using cationic surfactants (Fig. 3b), restoring ChT activity.…”

Universal hydrophilic coating of thermoplastic polymers currently used in microfluidics

“…There is thus a need to develop polymeric materials with a high hybridization signal-to-background ratio to enable the sensitive detection of proteins. A large number of techniques based on various principles such as optical absorption, refractive index changes, radiolabeling, electromechanical microbalances, and fluorescence markers (see (41) and references therein) have been used to provide information on protein adsorption.…”