Using steel plates attached with epoxy resin adhesive to strengthen prestressed reinforced concrete bridges has become a common method to increase bearing capacity in engineering because of the simple technology, low cost and good strengthening effects. The strengthening method of steel plates has been gradually applied to repair damaged bridges in practical engineering. After a cross-line box girder bridge was struck by a vehicle, the steel bars and concrete of a damaged girder were repaired and strengthened by steel plates, and then the ultimate bending bearing capacity was studied through a destructive test. The results of the destructive test were compared with those of an undamaged girder to verify the effect of the repair and strengthening of the damaged girder. The results showed that the actual flexural bearing capacity of the repaired girder strengthened by steel plates was 1.63 times the theoretical bearing capacity, 36.7% more than that of the damaged girder and 95.3% of that of an undamaged girder. The flexural cracking moment of the repaired girder strengthened by steel plates reached 66.3% of that of the undamaged girder. The maximum crack width decreased by 24.6%, and the maximum deflection increased by 2.7%, compared with the undamaged girder when the repaired girder strengthened by steel plates finally failed. Moreover, this method of attaching steel plates can increase the ductility of bridges and reduce the degree of cracking. Additionally, the actual safety factor of the repaired girder was greater than three, and it had a large safety reserve.