Experimental purification of paclitaxel from a complex mixture of taxanes using a simulated moving bed

Abstract

-A laboratory-scale simulated moving bed (SMB) was designed and tested for the separation of paclitaxel, a powerful anti-cancer agent known as Taxol @ , from impurities of a plant tissue culture (PTC) broth. The innovative strategy of a pseudo-binary model, where mixtures A and B were treated as single solutes A and B, was used in the linear standing wave analysis to fix the SMB operating parameters for a multicomponent and complex system. Linear standing wave design was used to specify the zone flow rates and the switching time for the laboratory-scale SMB unit, with two steps of separation. The SMB consists of four packed columns, where each column is 12.5 cm in length and 1.5 cm in diameter. Two sequential separation steps were used to recover paclitaxel from a small feed batch (less than one liter). Placlitaxel was recovered from the complex plant tissue culture broth in 82% yield and 72% purity.

Characterization of cytotoxic compound from mangrove derived fungi Irpex hydnoides VB4

“…It is estimated that there may be as many as 1 million different endophyte species; however, only handful of them are isolated and studied [16] . Of late the FDA (Food and Drug Administration) has approved Taxol from endophyte Taxomyces andreanae for the treatment of advanced breast cancer, lung cancer, and refractory ovarian cancer [17] . Anticancer activity of 14 [18] .…”

Endophytic Fungi as Alternative and Reliable Sources for Potent Anticancer Agents

“…Before, being isolated from the endophytic fungus Taxomyces andreanae, it was isolated for the irst time, from the bark of Taxus brevifolia, commonly called yew plant from the South American paciic. The drug was subsequently approved by the Food and Drug Administration (FDA), USA, for the treatment of selected cancers [46]. The diverse sources of taxol from endophytic fungi are shown in Table 1.…”

Current Understanding of Endophytes: Their Relevance, Importance, and Industrial Potentials

“…Additionally, the endophyte partner can extensively enhance plant resistance to biotic and abiotic challenges [79]. These beneficial features has been observed in infected plants exposed to several abiotic stress such as drought (166), heavy metal [80], high salinity [47], As well as biotic one including microbial infections [47], insect pest [81] and herbivores attack [91].…”

“…This compound impedes with the duplication of cancer cells, sinking or suspending their growth and spreading. FDA (Food and Drug Administration) has agreed Taxol is beneficial for the cure of advanced breast cancer, lungs cancer, refractory ovarian cancer [91]. Taxol was firstly isolated from the bark of tree belonging to taxus family (Taxus brevifolia), its main general resource [92].…”