Experimental Studies on Lateral Root Formation in Radish Seedling Roots. I. General Methods, Developmental Stages, and Spontaneous Formation of Laterals

Blakely, L. M.; Durham, Mark; Evans, Tony; Blakely, R. M.

doi:10.1086/337308

Cited by 112 publications

(73 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1A). This result confirms the work of previous investigators (Blakely et al, 1982). Furthermore, we found that when roots that had produced laterals in response to a particular concentration of exogenous auxin were subsequently exposed to a higher concentration of IAA, numerous supernumerary lateral roots spaced between existing ones were formed (Fig.…”

Section: Response Of Cultured Roots To Exogenous Iaasupporting

confidence: 92%

“…We used this method to cause a 1.7-fold increase in AAO in seedling radish roots grown in culture supplemented with 10 m CuSO 4 . We also measured the response of these roots to auxin by determining the lateral root frequency, which increases with auxin concentration (Blakely et al, 1982;Kerk, 1990) (Fig. 1A).…”

Section: Response Of Cultured Roots To Exogenous Iaamentioning

confidence: 99%

“…Using the radish (Raphanus sativus) root auxin bioassay system (Blakely et al, 1982), we found that increased levels of AAO in root segments in sterile culture decreased the response of radish roots to auxin. Moreover, if auxin is first reacted in vitro with AAO and then bioassayed, root cultures fail to exhibit a normal response.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Auxin Metabolism in the Root Apical Meristem

Kerk¹,

2000

View full text Add to dashboard Cite

Within the root meristem of flowering plants is a group of mitotically inactive cells designated the quiescent center (QC). Recent work links the quiescent state to high levels of the growth regulator auxin that accumulates in the QC via polar transport. This in turn results in elevated levels of the enzyme ascorbic acid oxidase (AAO), resulting in a reduction of ascorbic acid (AA) within the QC and mitotic quiescence. We present evidence for additional interactions between auxin, AAO, and AA, and report that, in vitro, AAO oxidatively decarboxylates auxin, suggesting a mechanism for regulating auxin levels within the QC. We also report that oxidative decarboxylation occurs at the root tip and that an intact root cap must be present for this metabolic event to occur. Finally, we consider how interaction between auxin and AAO may influence root development by regulating the formation of the QC.

show abstract

Section: Response Of Cultured Roots To Exogenous Iaasupporting

confidence: 92%

Section: Response Of Cultured Roots To Exogenous Iaamentioning

confidence: 99%

See 1 more Smart Citation

Auxin Metabolism in the Root Apical Meristem

Kerk¹,

2000

View full text Add to dashboard Cite

show abstract

“…Several lines of evidence indicate that auxin is necessary for the development of lateral roots. Application of IAA to growing plants stimulates lateral root development and lateral root elongation (Torrey, 1950;Blakely et al, 1982;Muday and Haworth, 1994). Conversely, growth of tomato roots on agar containing auxin-transport inhibitors, including NPA, decreases the number of lateral roots (Muday and Haworth, 1994).…”

mentioning

confidence: 99%

Inhibition of Auxin Movement from the Shoot into the Root Inhibits Lateral Root Development in Arabidopsis

1998

View full text Add to dashboard Cite

In roots two distinct polar movements of auxin have been reported that may control different developmental and growth events. To test the hypothesis that auxin derived from the shoot and transported toward the root controls lateral root development, the two polarities of auxin transport were uncoupled in Arabidopsis. Local application of the auxin-transport inhibitor naphthylphthalamic acid (NPA) at the root-shoot junction decreased the number and density of lateral roots and reduced the free indoleacetic acid (IAA) levels in the root and [ 3 H]IAA transport into the root. Application of NPA to the basal half of or at several positions along the root only reduced lateral root density in regions that were in contact with NPA or in regions apical to the site of application. Lateral root development was restored by application of IAA apical to NPA application. Lateral root development in Arabidopsis roots was also inhibited by excision of the shoot or dark growth and this inhibition was reversible by IAA. Together, these results are consistent with auxin transport from the shoot into the root controlling lateral root development.

show abstract

“…Many previous studies showed that auxin substantially affects the development of lateral roots (Blakely et al, 1982;MacIsaac et al, 1989;Malamy and Benfey, 1997;Lloret and Casero, 2002). However, auxin is a type of phytohormone, and only has a role as a signal that triggers the development of lateral root primordia.…”

mentioning

confidence: 97%