Experimental study for evaluation of the efficacy of a biofilm-embedded bacteria-based vaccine against Staphylococcus chromogenes-associated mastitis in sheep

Vasileiou, N.G.C.; Cripps, Peter; Ioannidi, K.S.; Katsafadou, Angeliki I.; Chatzopoulos, Dimitris; Barbagianni, M.S.; Tsioli, V.; Dermisiadou, Eleftheria; Karavanis, Emmanouel; Παπαδόπουλος, Νικόλαος; Lianou, Daphne Τ.; Mavrogianni, V.S.; Petinaki, Efthymia; Fthenakis, George C.

doi:10.1016/j.vetmic.2019.108480

Cited by 19 publications

(28 citation statements)

References 28 publications

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“…of S. chromogenes, as determined by the method of Miles and Misra [22]. The validity of these challenge models for inducing mastitis has been confirmed in many previous experimental studies [5,20,21,[23][24][25][26][27] by using clinical, bacteriological, cytological, ultrasonographic, proteomics and histological methods [6].…”

Section: Inoculation Proceduresmentioning

confidence: 75%

“…In experiment 2, ewes were inoculated with M. haemolytica or S. chromogenes strain 6684 (n = 3 and 3, respectively) directly into the gland cistern of the udder. Both strains had confirmed pathogenic action for the mammary gland of ewes [20,21]. All challenges were performed on the fifth day post-lambing.…”

Section: Experimental Overviewmentioning

confidence: 99%

“…The same technique was used to deposit 0.2 mL of sterile PBS into the contralateral teat duct of each ewe as a control. In experiment 2, the ewes were challenged as follows [5,21]. Initially, the teat was disinfected by using iodine povidone solution.…”

Section: Inoculation Proceduresmentioning

confidence: 99%

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Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes

et al. 2019

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The objective of the study was the investigation of the behaviour of cathelicidin-1 in the milk after experimental infection with two prominent bacterial pathogens (experiment 1: Mannheimia haemolytica, experiment 2: M. haemolytica and Staphylococcus chromogenes) as a potential early indicator for diagnosis of mastitis in sheep. In two experiments, after bacterial inoculation into the udder of ewes, bacteriological and cytological examinations of milk samples as well as proteomics examinations [two-dimensional gel electrophoresis analysis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) analysis] were performed sequentially. Cathelicidin-1 was detected and spot densities obtained from PDQuest v.8.0 were recorded. Associations were calculated between cell content and spot densities as well as between presence of mastitis in a mammary gland at a given time-point and detection of cathelicidin-1 in the respective milk sample. All inoculated mammary glands developed mastitis, confirmed by the consistent bacterial isolation from mammary secretion and increased leucocyte content therein. Spot density of cathelicidin-1 in samples from inoculated glands increased 3 h post-inoculation; spot density of cathelicidin-1 in samples from inoculated glands was higher than in samples from uninoculated controls. There was clear evidence of correlation between cell content and cathelicidin-1 spot densities in milk samples. There was significant association between presence of mastitis in the mammary gland and detection of cathelicidin-1 in the respective milk sample; overall accuracy was 0.818—this was significantly greater during the first 24 h post-challenge (0.903) than after the first day (0.704). In conclusion, detection of cathelicidin-1 in milk was significantly associated with presence of mastitis in ewes. The associations were stronger during the first 24 h post-infection than after the first day. Cathelicidin-1 has the advantage that it can be a non-specific biomarker, as simply a “positive”/“negative” assessment would be sufficient.

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Section: Inoculation Proceduresmentioning

confidence: 75%

Section: Experimental Overviewmentioning

confidence: 99%

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Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes

et al. 2019

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“…And this strong diffuse barrier hinders the penetration of antibiotics, especially positively charged ones [5,6], greatly improving the viability of bacteria [7]. The current studies found that P.a can use Quorum Sensing (QS) systems to regulate toxic factor formation named Las system (lasI/lasR) [8]. LasI encode the signal molecule 3-oxo-dodecanoyl homoserine lactone (3-OXO-C12-homoserinelactone, 3-O-C12-HSL) and transcriptional activator regulatory signaling molecules.…”

Section: Introductionmentioning

confidence: 97%

Study on the Mechanism of Application of Eucalyptus Volatile oil in Prevention and Treatment of Burn Wound Infections in Vitro

Yang¹,

Huang²,

Gao³

et al. 2020

Preprint

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Objective To investigate working mechanism of Eucalyptus volatile oil on the prevention and treatment of burn wound infections.Methods P.a biofilm model was used to investigate the effect of Eucalyptus volatile oil on bacterial biofilm. The expression of LasI mRNA in P.a was detected by RT-PCR.Results MIC test showed that the volatile oil of Eucalyptus Urophylla in a concentration of 20% or more could exert anti-bacterial effect. However, no zone of inhibition could be observed in the neither high nor low concentration of the Eucalyptus volatile oil. Scanning electron microscopy results showed a significant delay in volatile oil groups when compared with the control group. The expression of LasI mRNA in the volatile oil group was significantly lower than that in the control group. ConclusionAs Chinese medicine, the volatile oil of Eucalyptus Urophylla can affect the proliferation of P.a and biofilm formation by interfering with the expression of LasI, thus achieving the purpose of preventing and treating infection of burn patients.

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“…This barrier hinders the penetration of antibiotics, especially positively charged ones (5,6), greatly enhancing bacterial viability (7). To regulate the formation of toxic factors, PA can use the Quorum Sensing (QS) system named Las system (lasI/lasR) (8). LasI encodes the signal molecule 3-oxododecanoyl homoserine lactone (3-OXO-C12-homoserinelactone, 3-O-C12-HSL) and other signaling molecules that regulate transcriptional activators.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Eucalyptus Volatile Oil Application for Preventing and Treating Pseudomonas Aeruginosa Infection in Vitro

Yang¹,

Huang²,

Huang³

et al. 2020

Preprint

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Background The mechanism of eucalyptus volatile oil application on the prevention and treatment of burn infections caused by Pseudomonas aeruginosa (PA) was need to examined.Methods The effect of eucalyptus volatile oil on bacterial biofilm was investigated using a PA biofilm model. The expression of LasI mRNA in PA was detected by RT-PCR. Results The minimum inhibitory concentration test showed that the volatile oil of Eucalyptus urophylla in 20% concentration or more could exert an antibacterial effect. However, neither high nor low concentration of the eucalyptus volatile oil had a zone of inhibition. In scanning electron microscopy, the volatile oil group exhibited a significant delay compared with the control group. The volatile oil group also had a significantly lower LasI mRNA expression than the control group. Conclusion As a Chinese medicine, the volatile oil of E. urophylla can affect PA proliferation and biofilm formation by interfering with the expression of LasI, thereby successfully preventing and treating burn infection caused by PA.

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Experimental study for evaluation of the efficacy of a biofilm-embedded bacteria-based vaccine against Staphylococcus chromogenes-associated mastitis in sheep

Cited by 19 publications

References 28 publications

Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes

Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes

Study on the Mechanism of Application of Eucalyptus Volatile oil in Prevention and Treatment of Burn Wound Infections in Vitro

Mechanism of Eucalyptus Volatile Oil Application for Preventing and Treating Pseudomonas Aeruginosa Infection in Vitro

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