Experimental study on the heating/cooling and temperature uniformity performance of the microchannel temperature control device for nucleic acid PCR amplification reaction of COVID-19

Ling, Weisong; Zhou, Wei; Cui, Jiarong; Zheng, Shuilin; Wei, Qinglan; Chu, Xia

doi:10.1016/j.applthermaleng.2023.120342

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…For example, as reported in Ref. [17], the heating and cooling rates of the TEC Peltier element were about 8.3 °C/s and 8 °C/s, respectively. Recently, Ling et al [18] proposed a gradual thermal conductivity design to increase the temperature uniformity, and they reached average heating/cooling rates of 5.52 and 5.28 °C/s, respectively.…”

Section: Temperature Profilesmentioning

confidence: 57%

“…Demonstrating the limits of this method, their heating/cooling rates were around 7.2 and 6.12 • C/s, respectively. Compared with the heating and cooling rates of the PCR thermal cycler using the thermoelectric cooler effect or water-cooled or air-cooled effect [17,18] above, the device proposed in this work shows a significant improvement in both the heating and cooling processes. Figure 2A shows the temperature profile of 35 ultra-fast photothermal heating/cooling cycles within 7 min.…”

Section: Temperature Profilesmentioning

confidence: 97%

See 1 more Smart Citation

Photothermal-Based Multiplex Nested Digital PCR System for Rapid Detection of Foodborne Pathogens

Li,

Liang,

et al. 2024

Micromachines

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The rapid and sensitive detection of foodborne pathogens is crucial for ensuring food safety. Among virus testing methods, polymerase chain reaction (PCR) has served as the gold-standard technique in most food safety regulation organizations. However, to enhance the speed and efficiency of PCR, novel approaches are continually being explored. In this work, leveraging the photothermal effects and high thermal conductivity of gold nanoparticles, we have significantly improved the heating and cooling rates of thermal cycles, enabling ultra-fast PCR detection. Specifically, we present a pre-degassing multiplex digital PCR chip integrated with gold nanoparticles. We further developed a portable system with a light source for photothermal heating cycling, along with an optoelectronic sensor to analyze PCR amplification products after rapid thermal cycling. As proof of concept, the proposed chip and portable device was applied for the on-site detection of several types of foodborne pathogens, including Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella. The whole system could distinguish those pathogens within 20 min, showing good potential for the rapid detection of multiple types of foodborne pathogens.

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Section: Temperature Profilesmentioning

confidence: 57%

Section: Temperature Profilesmentioning

confidence: 97%

Photothermal-Based Multiplex Nested Digital PCR System for Rapid Detection of Foodborne Pathogens

Li,

Liang,

et al. 2024

Micromachines

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show abstract

“…Moreover, our calibration device encompasses features enabling simultaneous adjustment analyses involving both thermal parameters alongside composite optical parameters compared against single parameter adjustments, such as those focused solely on addressing heating/cooling rate within qPCR instruments [14,47]. Based on the simulation principles provided in this article, it is evident that the application of this device also includes the calibration of multiple-channel optical parameters and physical methods for analyzing melting curves.…”

Section: Discussionmentioning

confidence: 99%

“…Such defects can lead to differences in the optical path lengths between edge wells and central wells of qPCR instrument Block resulting in variations in the excitation light energy received by fluorescent groups in different wells, and ultimately affecting the reliability of experimental results, namely Ct values. Similarly, temperature differences between wells within the Block can also lead to such edge effects with even small optical or temperature differences between wells may be exponentially amplified during the amplification process, ultimately affecting experimental results [14,15].…”

Section: Of 22mentioning

confidence: 99%

Physical Simulation-Based Calibration for Quantitative Real-Time PCR

Zhu,

Liu,

Xiao

2024

Applied Sciences

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The fluorescence quantitative polymerase chain reaction (qPCR) instrument has been widely used in molecular biology applications, where the reliability of the qPCR performance directly affects the accuracy of its detection results. In this paper, an integrated, physics-based calibration device was developed to improve the accuracy and reliability of qPCR, realizing the calibration of qPCR instruments’ standard curve through physical simulations. With this calibration device, the collected temperature was used as the control signal to alter the fluorescence output, which allowed different probes to simulate the Ct values corresponding to samples with varying initial concentrations. The temperature and optical performance of this calibration device were tested, followed by a comparative analysis comparing the on-machine test results with standard substances to assess the linearity and uniformity of the Ct values of the measured qPCR instrument. It has been proven that this physical calibration device can effectively replace the biochemical standard substance to carry out comprehensive calibration of the temperature and optical parameters of the qPCR instrument and provide a more reliable method for the periodic calibration and quality control of the qPCR instrument. This contributes to the accuracy and reliability of fluorescence qPCR instruments in the field of molecular biology.

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Innovative strategies and approaches for enhancing performance in optical probe-based biosensors for point-of-care testing

Jin,

Zhang,

et al. 2024

TrAC Trends in Analytical Chemistry

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Experimental study on the heating/cooling and temperature uniformity performance of the microchannel temperature control device for nucleic acid PCR amplification reaction of COVID-19

Cited by 4 publications

References 29 publications

Photothermal-Based Multiplex Nested Digital PCR System for Rapid Detection of Foodborne Pathogens

Photothermal-Based Multiplex Nested Digital PCR System for Rapid Detection of Foodborne Pathogens

Physical Simulation-Based Calibration for Quantitative Real-Time PCR

Innovative strategies and approaches for enhancing performance in optical probe-based biosensors for point-of-care testing

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