Experimental study on the interaction between lead and serum albumin

Abstract

The interaction between lead (Pb 2+) and bovine serum albumin (BSA) was studied in the temperature range of 10-40 °C through metal-titration experiments under equilibrium conditions. Lead binding to BSA was monitored by measuring the intensity changes in the difference absorption band at 243 nm following the addition of increasing amounts of lead ions to an aqueous solution at constant protein concentration (2.5 × 10 -5 M). The equilibrium binding data were found to be well described by a Langmuir-type equation, with an almost temperature-independent saturation value. According to the estimated δH 0 and δS 0, the binding of Pb 2+ to BSA was both enthalpy-driven and entropy-driven, with the entropic contribution providing the major driving force. © 2012 Curtin University of Technology and John Wiley & Sons, Ltd

Preparation and Characterization of Magnetically Responsive Biosorbents from Coffee Industry Residues

“…After 3 h, the adsorbent was separated from the suspension using a permanent magnet and the clear solution was assayed for dye content.The initial dye concentration was varied between 25 and 1000 mg L -1 . The concentration of methylene blue in the liquid was determined spectrophotometrically at 665 nm using a molar extinction coefficient of 4.73 × 10 3 M -1 cm -1 [11].…”

Methodologies of Removal of Heavy Metals from Contaminated Water Using Various Waste Materials: A Comprehensive Review

“…Zuorro and Lavecchia [37] investigated the use of black tea waste adsorbent. The tea waste was washed repeatedly to remove all the color and was dried in the oven for 24 h at 60 °C.…”

“…As the solution concentration of Pb 2+ ions increased from 350 to 1750 mg L -1 , the removal increased from 94.4 to 98.6%. Surface adsorption, microprecipitation, chemisorption, ion exchange, and complexation were the mechanisms for metal uptake [37].…”

“…Percentage removal of (a) cadmium, (b) copper (c), chromium, (d) nickel, (e) lead, and (f) zinc by various waste materials 35–70.…”

“…The percentage removal of heavy metals using various waste materials is reported in Tab. 5 29, 35–70.…”