Experimental Study on the Transmission of Sendai Virus in Specific Pathogen-free Mice

Iida, Tsuyoshi

doi:10.1099/0022-1317-14-1-69

Cited by 14 publications

(11 citation statements)

References 10 publications

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“…Thus, while i.m. vaccination may afford an attractive vehicle to reduce pathogenesis promoted by infection in the lungs after natural exposure, just as it does in influenza virus infection (54), it may not fully protect from reinfection and may allow limited transmission, which has been linked to virus shedding in the URT (24,55).…”

Section: Discussionmentioning

confidence: 99%

Relationships among Dissemination of Primary Parainfluenza Virus Infection in the Respiratory Tract, Mucosal and Peripheral Immune Responses, and Protection from Reinfection: a Noninvasive Bioluminescence-Imaging Study

Burke

Hurwitz

et al. 2015

J Virol

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Respiratory paramyxoviruses such as respiratory syncytial virus (RSV) and human parainfluenza virus type 1 (HPIV1) to HPIV4 infect virtually all children by the age of 2 to 5 years, leading to partial but incomplete protection from reinfection. Here, we used luciferase-expressing reporter Sendai viruses (the murine counterpart of HPIV1) to noninvasively measure primary infection, immune responses, and protection from reinfection by either a lethal challenge or natural transmission in living mice. Both nonattenuated and attenuated reporter Sendai viruses were used, and three inoculation strategies were employed: intramuscular (i.m.), intranasal (i.n.) at a low dose and low volume, and i.n. at a high dose and high volume. High-dose, high-volume i.n. inoculation resulted in the highest levels of antibody responses and protection from reinfection. Low-dose, low-volume i.n. inoculation afforded complete protection from contact transmission and protection from morbidity, mortality, and viral growth during lethal challenge. i.m. inoculation was inferior to i.n. inoculation at inducing antibody responses and protection from challenge. For individual mice and across groups, the levels of serum binding and neutralizing antibody responses correlated with primary infection and protection from reinfection in the lungs. Contact transmission, the predominant mode of parainfluenza virus transmission, was modeled accurately by direct i.n. inoculation of Sendai virus at a low dose and low volume and was completely preventable by i.n. vaccination of an attenuated virus at a low dose and low volume. The data highlight differences in infection and protection from challenge in the upper versus lower respiratory tract and bear upon live attenuated vaccine development. IMPORTANCEThere are currently no licensed vaccines against HPIVs and human RSV (HRSV), important respiratory pathogens of infants and children. Natural infection leads to partial but incomplete protective immunity, resulting in subsequent reinfections even in the absence of antigenic drift. Here, we used noninvasive bioluminescence imaging in a mouse model to dissect relationships among (i) the mode of inoculation, (ii) the dynamics of primary infection, (iii) consequent immune responses, and (iv) protection from high-dose, high-volume lethal challenge and contact transmission, which we find here to be similar to that of a mild low-dose, low-volume upper respiratory tract (URT)-biased infection. Our studies demonstrate the superiority of i.n. versus i.m. vaccination in protection against both lethal challenge and contact transmission. In addition to providing correlates of protection that will assist respiratory virus vaccine development, these studies extend the development of an increasingly used technique for the study of viral infection and immunity, noninvasive bioluminescence imaging.H uman respiratory syncytial virus (HRSV), human metapneumovirus (HMPV), and human parainfluenza virus type 1 (HPIV1) to HPIV4 are leading viral causes of pediatric hospitaliza...

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Section: Discussionmentioning

confidence: 99%

Relationships among Dissemination of Primary Parainfluenza Virus Infection in the Respiratory Tract, Mucosal and Peripheral Immune Responses, and Protection from Reinfection: a Noninvasive Bioluminescence-Imaging Study

Burke

Hurwitz

et al. 2015

J Virol

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“…In other experiments, RFVL mice from our own SPF colony were used. These mice were housed as described previously (Iida, 1972).…”

Section: Methodsmentioning

confidence: 99%

“…Four-week-old female SPF mice were infected with Sendai virus by contact (Iida, 1972) and housed for 8 weeks in isolation. Mating was then as above.…”

Section: Methodsmentioning

confidence: 99%

Transmission of Maternal Antibodies to Sendai Virus in Mice and its Significance in Enzootic Infection

Iida

Tajima

Murata

1973

Journal of General Virology

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SUMMARYMaternal antibodies to Sendai virus appeared to be transferred to newborn mice to a greater extent via colostrum and gastro-intestinal tract than via the placenta. Serum antibody reached a maximum within a week of birth and remained high for a few weeks before decreasing gradually. The immunoelectrophoretic analysis of serum globulins of suckling mice demonstrated IgG1 and IgG~ but no IgA, and suggested a selective transport of maternal globulins through the gastrointestinal epithelium of suckling mice. The maternal antibody effectively protected one-day-old newborn mice against intranasal challenge by a lethal dose of Sendal virus, but was less effective against the contact infection of 3-week-old mice. Thus, passive immunity appeared to be brief. Enzootic infections with Sendai virus are discussed on the basis of these experiments.

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“…Paramyxoviruses are responsible for several respiratory diseases in both animals and humans (particularly in infants). Airborne transmission of several paramyxoviruses, including Newcastle disease virus, has been demonstrated (Iida 1972;Li et al 2009;Lorenz and Albrecht 1980). Thus this family is an interesting model for evaluating the potential of neuraminidase activity for detection of airborne viruses of both human and animal importance.…”

Section: A Good Model For Studying the Neuraminidasementioning

confidence: 99%

Neuraminidase Activity as a Potential Enzymatic Marker for Rapid Detection of Airborne Viruses

Turgeon

McNicoll

Toulouse

et al. 2011

Aerosol Science and Technology

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Viruses offer a limited range of targets for their detection. To date, PCR and RT-PCR have been widely used for detection of viruses. In the case of environmental air sampling, the ability to detect a broad range of viruses would constitute a significant advantage for preventing outbreaks of airborne-transmitted viral infections. Given that neuraminidase is found on some respiratory virus Address correspondence to Caroline Duchaine, Ph.D., Hôpital Laval, 2725 Chemin Ste-Foy, Québec, Canada, G1V 4G5. E-mail: Caroline.Duchaine@bcm.ulaval.ca species of medical or agricultural importance, this enzyme could theoretically be used to detect several different airborne viruses in a single assay. The aim of the present study was to evaluate the potential of neuraminidase activity as a marker for rapid detection of airborne viruses. We first validated the use of a low-pathogenic strain of Newcastle disease virus (NDV) as a model airborne virus. Our findings revealed that neuraminidase activity-based assays are almost as sensitive as RT-PCR assays currently used for detection of NDV. We also validated the utilization of a neuraminidase substrate specific to viral neuraminidase. Experiments conducted in a controlled chamber demonstrated that the neuraminidase activity is preserved after aerosolization, air sampling using impingement and handling. Finally, we tested our method with swine barn air samples. Our results demonstrate that neuraminidase activitybased assays are suitable for detection of viruses in air samples.

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Experimental Study on the Transmission of Sendai Virus in Specific Pathogen-free Mice

Cited by 14 publications

References 10 publications

Relationships among Dissemination of Primary Parainfluenza Virus Infection in the Respiratory Tract, Mucosal and Peripheral Immune Responses, and Protection from Reinfection: a Noninvasive Bioluminescence-Imaging Study

Relationships among Dissemination of Primary Parainfluenza Virus Infection in the Respiratory Tract, Mucosal and Peripheral Immune Responses, and Protection from Reinfection: a Noninvasive Bioluminescence-Imaging Study

Transmission of Maternal Antibodies to Sendai Virus in Mice and its Significance in Enzootic Infection

Neuraminidase Activity as a Potential Enzymatic Marker for Rapid Detection of Airborne Viruses

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