Experimental tinea unguium model to assess topical antifungal agents using the infected human nail with dermatophyte in vitro

Nakashima, Takuji; Nozawa, Akira; Ito, Takao; Majima, Toshiro

doi:10.1007/s10156-002-0192-8

Cited by 14 publications

(8 citation statements)

References 24 publications

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“…Contributions have been made in the determination of in vivo effects of antifungal agents in combination with the previous medium has been suggested. Already a new method against onychomycosis was proposed by Nakashima et al [91]. This approach is expected to be useful for evaluating the preliminary antifungal effect.…”

Section: Approach To Accurately Evaluate the Antifungal Agent In Vivomentioning

confidence: 99%

Animal Model of Dermatophytosis

Shimamura

Kubota

Shibuya

2012

Journal of Biomedicine and Biotechnology

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Dermatophytosis is superficial fungal infection caused by dermatophytes that invade the keratinized tissue of humans and animals. Lesions from dermatophytosis exhibit an inflammatory reaction induced to eliminate the invading fungi by using the host's normal immune function. Many scientists have attempted to establish an experimental animal model to elucidate the pathogenesis of human dermatophytosis and evaluate drug efficacy. However, current animal models have several issues. In the present paper, we surveyed reports about the methodology of the dermatophytosis animal model for tinea corporis, tinea pedis, and tinea unguium and discussed future prospects.

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Section: Approach To Accurately Evaluate the Antifungal Agent In Vivomentioning

confidence: 99%

Animal Model of Dermatophytosis

Shimamura

Kubota

Shibuya

2012

Journal of Biomedicine and Biotechnology

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“…In addition, antifungal activity may be reduced when there is affinity of the drug for keratin . For these reasons, it is believed that a high concentration of the drug is required to fight onychomycosis …”

Section: Alternative Methods That Simulate Fungal Infectionsmentioning

confidence: 99%

Fungal infection models: Current progress ofex vivomethods

Quatrin

Lana

Kaminski

2019

Mycoses

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| INTRODUC TI ONThe principles developed by Russell and Burch in 1959, concerning the use of animals in research, have been updated in recent decades, and cover the use of tissues from dead animals as alternatives to in vivo methods. 1 In vivo tests generally involve a large number of specimens to obtain representative results, and the well-being of these animals has become an ethical issue to be respected. 2 The term ex vivo refers to the use of living tissues, which are removed from an organism in vivo, for the application in experiments that will be performed in an artificial environment. 3The murine models used to evaluate the therapeutic profile of new antifungal agents are addressed in several studies, such as induction of keratitis, onychomycosis, and systemic, vaginal, pulmonary and cutaneous infections. 4-9 However, it is important to maintain a balance between qualified science and animal well-being, using rational and ethical ways of research. In this scenario, ex vivo models that simulate fungal infections in nails, mucous membranes and skin are promising alternatives that have been showing considerable results. [10][11][12] In order to demonstrate new perspectives in the evaluation of antifungal therapy, we will approach a critical review of the main ex vivo techniques used in the simulation and treatment of mycoses, providing an adaptation of the widely used in vivo methods. | ME THODS | Data source SummaryExperimental alternative ex vivo models that simulate infectious processes in vivo are of fundamental importance for the evaluation of new drugs, since in some cases, their execution does not depend on the approval of an ethics committee in research.Although studies using alternative infectious models to evaluate the efficacy of antifungal molecules have been increasingly described and reported, there is no critical consensus that establishes the most appropriate ones regarding the type of infection. Numerous studies contemplate ex vivo protocols of fungal infections on nails, corneas, dentinal tubules and skin and reveal counterpoints and concordances not yet finely confronted. In this minireview, we propose a critical analysis of the main ex vivo models of fungal infections for the evaluation of new antifungal candidates for both topical and systemic use, as opposed to the advantages and disadvantages of the traditional in vivo models employed in preclinical research. K E Y W O R D S dentinal tubule infections, ex vivo models, mucosal infections, onychomycosis, skin infection, systemic infections | 861 MACIEL QUATRIN ET AL. How to cite this article: Maciel Quatrin P, Flores Dalla Lana D, Andrzejewski Kaminski TF, Meneghello Fuentefria A. Fungal infection models: Current progress of ex vivo methods.

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“…Infected human nail by [50] In this in vitro model, a nail plate is placed in a Sabouraud dextrose agar, which has been pre-inoculated with a T. mentagrophytes conidial suspension. An O-ring is attached with silicon bond on the dorsal surface of the nail.…”

Section: In Vitro Efficacy Evaluationmentioning

confidence: 99%