Experimental Tuberculosis and Its Chemotherapy

The Experimental Evaluation of ANTI‐T.B. Drugs

“…in the case of sulphetrone which is active in guinea-pigs (Brownlee & Kennedy, 1948) and inactive in mice (Callow et al, 1947). This fact has been confirmed by Croshaw & Dickinson (1950).…”

“…Dubos' medium (Dubos & Davis, 1946) containing Tween 80, although giving results in a shorter time, is probably not ideal as it has been shown by Youmans & Youmans (1948) that Tween 80 may increase or reduce the bacteriostatic effect of some drugs. Croshaw & Dickinson (1950) found that results in a modified Dubos medium (Forrest, Hart & Walker, 1947) were usually considerably lower than with Long's medium and surface growth.…”

“…I n earlier work with mice, Croshaw & Dickinson (1950), using both the intraperitoneal and intravenous routes of infection, found there were few specific deaths and wide variation in lung involvement. This variation was so great with the strains of mice used that this test appeared useless for the assessment of drug activity unless very large groups of mice were used.…”

“…Of over 1,000 new compounds tested in vitro by Croshaw & Dickinson (1950) a number having high activity even in the presence of 10% of serum were tested in vivo and found inactive. I n most cases, however, the maximum tolerated amount of drug for animals was fairly low.…”

“…Although this method is quick and requires little drug, it is not widely used. Croshaw & Dickinson (1950) used the chick embryo method in which human tubercle bacilli were inoculated either on to the chorioallantoic membrane or into the yolk-sac but never obtained a uniform infection as judged by the macroscopic appearance of lesions.…”