Explant Developmental State and Shoot Formation in Pinus radiata Cotyledons

Aitken-Christie, J.; Singh, Adya P.; Horgan, Kathryn; Thorpe, Trevor A.

doi:10.1086/337515

Cited by 45 publications

(25 citation statements)

References 13 publications

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“…4b, 5a). This basal shoot zone in contact with the culture medium is a sink for endogenous phytohormones and lesion-derived stress compounds that stimulate tissue dedifferentiation and formation of cell meristematic centers (Washer et al 1977;Aitken-Christie et al 1985). These centers with starch-rich cells continued to grow throughout the culture period surrounding primordial tracheary elements (Fig.…”

Section: P Pinaster Plantlets Shoots and Co-cultures Structurementioning

confidence: 99%

In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease

Faria

Sena

Silva

et al. 2015

Planta

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Main conclusion Co-cultures of Pinus pinaster with Bursaphelenchus xylophilus were established as a biotechnological tool to evaluate the effect of nematotoxics addition in a host/parasite culture system.The pinewood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), was detected for the first time in Europe in 1999 spreading throughout the pine forests in Portugal and recently in Spain. Plant in vitro cultures may be a useful experimental system to investigate the plant/nematode relationships in loco, thus avoiding the difficulties of field assays. In this study, Pinus pinaster in vitro cultures were established and compared to in vivo 1 year-old plantlets by analyzing shoot structure and volatiles production. In vitro co-cultures were established with the PWN and the effect of the phytoparasite on in vitro shoot structure, water content and volatiles production was evaluated. In vitro shoots showed similar structure and volatiles production to in vivo maritime pine plantlets. The first macroscopic symptoms of PWD were observed about 4 weeks after in vitro co-culture establishment. Nematode population in the culture medium increased and PWNs were detected in gaps of the callus tissue and in cavities developed from the degradation of cambial cells. In terms of volatiles main components, plantlets, P. pinaster cultures, and P. pinaster with B. xylophilus co-cultures were all b-and a-pinene rich. Cocultures may be an easy-to-handle biotechnological approach to study this pathology, envisioning the understanding of and finding ways to restrain this highly devastating nematode.

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Section: P Pinaster Plantlets Shoots and Co-cultures Structurementioning

confidence: 99%

In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease

Faria

Sena

Silva

et al. 2015

Planta

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“…4 Since the first experiment, there has been an indication that the percentage of embryos forming good meristematic tissue on LP5 medium increased from 20% (Tab. 2) to 31% (Tab.…”

Section: Multiplication Of Meristematic Tissue On Four Mediamentioning

confidence: 99%

“…Most studies with conifers have shown that shoots are formed from meristematic tissue, meristemoids, meristematic centers, or domes, and it has been suggested that Pinus radiata shoots can only be induced from this type of tissue (4). With radiata pine, formation of meristematic tissue is a prerequisite for first-generation adventitious shoots (1).…”

Section: Introductionmentioning

confidence: 99%

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Multiplication of Meristematic Tissue: A New Tissue Culture System for Radiata Pine

Aitken-Christie

Singh

Davies

1988

Genetic Manipulation of Woody Plants

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103

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“…menziesii (Cheah and Cheng 1978). A prerequisite for the location of this adventitious meristematic region on the embryonic tissue is that the cells must be competent for differentiation (Aitken-Christie et al 1985). In P/.…”

Section: Discussionmentioning

confidence: 99%

Adventitious bud development in embryonic tissue of Pinus caribaea and Pseudotsuga menziesii

Skidmore

1988

Trees

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Summary. Adventitious bud formation was induced on detached cotyledons and on cotyledons attached to excised embryos of Pinus caribaea and Pseudotsuga menziesii. The embryonic tissue was exposed to the cytokinin 6-benzyl amino purine contained within an agar medium. This exposure resulted in the formation of a meristematic zone, involving both epidermal and sub-epidermal cells, and then nodules on the tissue surface. Some of these nodules were induced to differentiate into bud primordia, and thence shoots, following exposure to a combination of auxin (IAA) and cytokinin. Shoots were produced over the entire surface of detached cotyledons of Ps. menziesii but predominantly on the adaxial surfaces of detached cotyledons of Pi. caribaea and the tips and adaxial surfaces of the cotyledons on the entire embryos of both species. Thus, inter-specific differences in the distribution of competent areas for adventitious bud production were detected in embryos.

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Explant Developmental State and Shoot Formation in Pinus radiata Cotyledons

Cited by 45 publications

References 13 publications

In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease

In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease

Multiplication of Meristematic Tissue: A New Tissue Culture System for Radiata Pine

Adventitious bud development in embryonic tissue of Pinus caribaea and Pseudotsuga menziesii

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