Exploitation of the mycoparasitic fungus Pythium oligandrum in plant protection - A review

Brožová, Jana

doi:10.17221/4818-pps

Cited by 43 publications

(20 citation statements)

References 18 publications

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“…The expanding knowledge of the mechanisms underlying the mode of action of P. oligandrum will open new avenues of potential applications. Currently, only one P. oligandrum-based agricultural product, Polyversum (Brozova, 2002), is registered but it is likely that other P. oligandrumderived products will be commercialized in the near future.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Pythium oligandrum: an example of opportunistic success

et al. 2012

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Pythium oligandrum, a non-pathogenic soil-inhabiting oomycete, colonizes the root ecosystem of many crop species. Whereas most members in the genus Pythium are plant pathogens, P. oligandrum distinguishes itself from the pathogenic species by its ability to protect plants from biotic stresses in addition to promoting plant growth. The success of P. oligandrum at controlling soilborne pathogens is partly associated with direct antagonism mediated by mycoparasitism and antimicrobial compounds. Interestingly, P. oligandrum has evolved with specific mechanisms to attack its prey even when these belong to closely related species. Of particular relevance is the question of how P. oligandrum distinguishes between self-and non-self cell wall degradation during the mycoparasitic process of pathogenic oomycete species. The ability of P. oligandrum to enter and colonize the root system before rapidly degenerating is one of the most striking features that differentiate it from all other known biocontrol fungal agents. In spite of this atypical behaviour, P. oligandrum sensitizes the plant to defend itself through the production of at least two types of microbe-associated molecular patterns, including oligandrin and cell wall protein fractions, which appear to be closely involved in the early events preceding activation of the jasmonic acid-and ethylene-dependent signalling pathways and subsequent localized and systemic induced resistance. The aim of this review is to highlight the expanding knowledge of the mechanisms by which P. oligandrum provides beneficial effects to plants and to explore the potential use of this oomycete or its metabolites as new disease management strategies.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Pythium oligandrum: an example of opportunistic success

et al. 2012

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“…Presently, different species of Trichoderma are used successfully to control different plant pathogens (Vinalea et al 2008). Colony interaction between non-pathogenic and pathogenic fungi for determination of antagonism of fungi isolated from different habitats including rhizosphere are studied (Skidmore and Dickinson 1976, Bashar and Rai 1994, Brozova 2002, and Prince et al 2011. Different rhizodeposition of plant invites beneficial microbiota around the rhizosphere.…”

Section: Introductionmentioning

confidence: 99%

Antagonistic potential of rhizosphere fungi against leaf spot and fruit rot pathogens of brinjal

Aktar

Hossain²,

Bashar

2015

Bangladesh J. Bot.

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Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested in opposition to six pathogenic fungi viz., Colletotrichum sp., Curvularia lunata, Fusarium moniliforme, F. oxysporum, F. semitectum and Phomopsis sp. isolated from different leaf spots and fruit rots of brinjal. Out of seven soil fungi, Trichoderma harzianum was found most effective to control the growth of all the test fungi in the study of colony interactions and effects of volatile and non-volatile metabolites. This fungus may be exploited commercially to biocontrol the diseases.

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“…Pythium oligandrum was commercialised as a biocontrol agent for the protection of a wide variety of plant species under license as Polygardron in the Slovak Republic (Brozova 2002). It is also an endophyte capable of colonising the root rhizosphere of many crop plants (Martin & Hancock 1987).…”

Section: Introductionmentioning

confidence: 99%

The oomycete Pythium oligandrum expresses putative effectors during mycoparasitism of Phytophthora infestans and is amenable to transformation

2012

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The oomycete Pythium oligandrum is a mycoparasitic biocontrol agent that is able to antagonise several plant pathogens, and can promote plant growth. In order to test the potential usefulness of P. oligandrum as a biocontrol agent against late blight disease caused by the oomycete Phytophthora infestans, we investigated the interaction between P. oligandrum and Ph. infestans using the green fluorescent protein (GFP) as a reporter gene. A CaCl(2) and polyethylene-glycol-based DNA transformation protocol was developed for P. oligandrum and transformants constitutively expressing GFP were produced. Up to 56 % of P. oligandrum transformants showed both antibiotic resistance and fluorescence. Mycoparasitic interactions, including coiling of P. oligandrum hyphae around Ph. infestans hyphae, were observed with fluorescent microscopy. To gain further insights into the nature of P. oligandrum mycoparasitism, we sequenced 2376 clones from cDNA libraries of P. oligandrum mycelium grown in vitro, or on heat-killed Ph. infestans mycelium as the sole nutrient source. 1219 consensus sequences were obtained including transcripts encoding glucanases, proteases, protease inhibitors, putative effectors and elicitors, which may play a role in mycoparasitism. This represents the first published expressed sequence tag (EST) resource for P. oligandrum and provides a platform for further molecular studies and comparative analysis in the Pythiales.

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Exploitation of the mycoparasitic fungus Pythium oligandrum in plant protection - A review

Cited by 43 publications

References 18 publications

Pythium oligandrum: an example of opportunistic success

Pythium oligandrum: an example of opportunistic success

Antagonistic potential of rhizosphere fungi against leaf spot and fruit rot pathogens of brinjal

The oomycete Pythium oligandrum expresses putative effectors during mycoparasitism of Phytophthora infestans and is amenable to transformation

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