Exploiting the Acidic Extracellular pH: Evaluation of Streptococcus salivarius M18 Postbiotics to Target Cancer Cells

Karaçam, Sevinç; Tunçer, Sinem

doi:10.1007/s12602-021-09806-3

Cited by 11 publications

(8 citation statements)

References 94 publications

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“…The range of specific probiotic applications of BLIS M18 have largely been prompted by idiosyncrasies of its BLIS spectrum ( Supplementary Table B ). Successful outcomes have been reported not only for its impact on Cariogram index ( Di Pierro et al, 2015b ; Tandelilin et al, 2018 ; Poorni et al, 2022 ), reduction of gingivitis/periodontitis ( Scariya et al, 2015 ) and halitosis ( Benic et al, 2019 ; Yoo et al, 2020 ) and modulation of black staining plaque ( Gobbi et al, 2020 ), but also for its unanticipated postbiotic activity against colon cancer cells ( Karaçam and Tunçer, 2022 ) and the important Gram-negative pathogens P. aeruginosa and Klebsiella pneumoniae ( Tunçer and Karaçam, 2020 ).…”

Section: Blis-producing Streptococcus Salivarius A...mentioning

confidence: 99%

Beneficial modulation of human health in the oral cavity and beyond using bacteriocin-like inhibitory substance-producing streptococcal probiotics

Tagg¹,

Harold²,

Jain³

et al. 2023

Front. Microbiol.

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The human oral cavity contains a diversity of microbial habitats that have been adopted and adapted to as homeland by an amazingly heterogeneous population of microorganisms collectively referred to as the oral microbiota. These microbes generally co-habit in harmonious homeostasis. However, under conditions of imposed stress, as with changes to the host’s physiology or nutritional status, or as a response to foreign microbial or antimicrobial incursions, some components of the oral “microbiome” (viz. the in situ microbiota) may enter a dysbiotic state. This microbiome dysbiosis can manifest in a variety of guises including streptococcal sore throats, dental caries, oral thrush, halitosis and periodontal disease. Most of the strategies currently available for the management or treatment of microbial diseases of the oral cavity focus on the repetitive “broad sweep” and short-term culling of oral microbe populations, hopefully including the perceived principal pathogens. Both physical and chemical techniques are used. However, the application of more focused approaches to the harnessing or elimination of key oral cavity pathogens is now feasible through the use of probiotic strains that are naturally adapted for oral cavity colonization and also are equipped to produce anti-competitor molecules such as the bacteriocins and bacteriocin-like inhibitory substances (viz BLIS). Some of these probiotics are capable of suppressing the proliferation of a variety of recognized microbial pathogens of the human mouth, thereby assisting with the restoration of oral microbiome homeostasis. BLIS K12 and BLIS M18, the progenitors of the BLIS-producing oral probiotics, are members of the human oral cavity commensal species Streptococcus salivarius. More recently however, a number of other streptococcal and some non-streptococcal candidate oral probiotics have also been promoted. What is becoming increasingly apparent is that the future for oral probiotic applications will probably extend well beyond the attempted limitation of the direct pathological consequences of oral microbiome dysbiosis to also encompass a plethora of systemic diseases and disorders of the human host. The background to and the evolving prospects for the beneficial modulation of the oral microbiome via the application of BLIS-producing S. salivarius probiotics comprises the principal focus of the present review.

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Section: Blis-producing Streptococcus Salivarius A...mentioning

confidence: 99%

Beneficial modulation of human health in the oral cavity and beyond using bacteriocin-like inhibitory substance-producing streptococcal probiotics

Tagg¹,

Harold²,

Jain³

et al. 2023

Front. Microbiol.

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“…Some PMs show anti-proliferative effects when added in specific microenvironments. For example, PM from the cell free supernatant of Streptococcus salivarius M18 cultured on prebiotic inulin displayed potent inhibitory effect on colon cancer cell proliferation through increasing extracellular acidity within the tumor ( 129 ). Postbiotics like SCFA and tryptophan have shown to downregulate both the adaptive and humoral immunity in inflammatory conditions such as inflammatory bowel diseases ( 130 ).…”

Section: The Effects Of Dietary Interventions On Colorectal Cancermentioning

confidence: 99%

Emerging Evidence on the Effects of Dietary Factors on the Gut Microbiome in Colorectal Cancer

et al. 2021

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Dietary factors have important role in modulating the gut microbiome, which in-turn regulates the molecular events in colonic mucosa. The composition and resulting metabolism of the gut microbiome are decisive factors in colorectal cancer (CRC) tumorigenesis. Altered gut microbiome is associated with impaired immune response, and the release of carcinogenic or genotoxic substances which are the major microbiome-induced mechanisms implicated in CRC pathogenesis. Diets low in dietary fibers and phytomolecules as well as high in red meat are important dietary changes which predispose to CRC. Dietary fibers which reach the colon in an undigested form are further metabolized by the gut microbiome into enterocyte friendly metabolites such as short chain fatty acid (SCFA) which provide anti-inflammatory and anti-proliferative effects. Healthy microbiome supported by dietary fibers and phytomolecules could decrease cell proliferation by regulating the epigenetic events which activate proto-oncogenes and oncogenic pathways. Emerging evidence show that predominance of microbes such as Fusobacterium nucleatum can predispose the colonic mucosa to malignant transformation. Dietary and lifestyle modifications have been demonstrated to restrict the growth of potentially harmful opportunistic organisms. Synbiotics can protect the intestinal mucosa by improving immune response and decreasing the production of toxic metabolites, oxidative stress and cell proliferation. In this narrative review, we aim to update the emerging evidence on how diet could modulate the gut microbial composition and revive colonic epithelium. This review highlights the importance of healthy plant-based diet and related supplements in CRC prevention by improving the gut microbiome.

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“…Studying cell growth, development patterns, and functions in vitro by culturing cells in a simulated vivo environment is an important tool in modern life sciences, and pH detection is of great value in the cellular microenvironment. , Hydrogen ion, which directly reflects the pH of the cellular microenvironment, plays an important role in cell growth, development, and division. , In general, the pH of the cellular environment is dynamically balanced within a range of 7–9, while the pH of cancer cells can reach 5.5 . Various methods have been developed to detect pH, ranging from traditional pH sensors to new pH detection devices including optical sensors, electrochemical sensors, visual sensors, and so forth.…”

Section: Introductionmentioning

confidence: 99%

“…3,4 In general, the pH of the cellular environment is dynamically balanced within a range of 7−9, while the pH of cancer cells can reach 5.5. 5 Various methods have been developed to detect pH, ranging from traditional pH sensors to new pH detection devices 6 including optical sensors, 7 electrochemical sensors, 8 visual sensors, 9 and so forth. Common commercial pH sensors rely on the electrochemical principle to detect the potential of the solution, which is dependent on the hydrogen ion concentration of the solution.…”

Section: ■ Introductionmentioning

confidence: 99%

Liquid Metal pH Morphology Sensor Used for Biological Microenvironment Detection

Wang

Liang

et al. 2022

Anal. Chem.

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pH is one of the important parameters of a biological microenvironment, which is closely related to cell growth, development, vitality, division, and differentiation. Monitoring the pH of a microenvironment is helpful to monitor the cell metabolism as well as to understand the cellular life cycle. The sensitivity of liquid metals (LMs) to hydrogen ions has aroused our interest. Here, we propose a novel but facile pH sensor using liquid gallium (LM for short) droplet morphological change as the readout. The pH sensing characteristics of the LM droplet were examined, especially the shape response. LM can form solid native oxide skin rapidly in oxygenated solution, and the oxide layer will be removed in acidic or alkaline solutions, which will cause a great change in surface tension. The phenomenon is the change of LM morphology from macroscopic observation. We explored the electrochemical characteristics of LM at different pH values, explained the mechanism of surface change, and calibrated the relationship curve between LM morphology and pH and the interference of impurity ions on the sensor. Finally, we proposed a detection algorithm for the LM pH morphology sensor and tried to automatically detect pH with a mobile app, which was applied to the pH detection of cell culture solution. We believe that the response characteristics of LM to hydrogen ions have great potential in microenvironment detection.

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Exploiting the Acidic Extracellular pH: Evaluation of Streptococcus salivarius M18 Postbiotics to Target Cancer Cells

Cited by 11 publications

References 94 publications

Beneficial modulation of human health in the oral cavity and beyond using bacteriocin-like inhibitory substance-producing streptococcal probiotics

Beneficial modulation of human health in the oral cavity and beyond using bacteriocin-like inhibitory substance-producing streptococcal probiotics

Emerging Evidence on the Effects of Dietary Factors on the Gut Microbiome in Colorectal Cancer

Liquid Metal pH Morphology Sensor Used for Biological Microenvironment Detection

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