Exploration and practice on systematic identification strategy of traditional Chinese medicine prescriptions by high-performance thin-layer chromatography—with Daqinjiao decoction as an example

Chen, Ruibiao; Liang, Shengwang; Wang, Shumei; Xie, Yonghong

doi:10.1007/s00764-022-00166-4

Cited by 5 publications

(3 citation statements)

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“…Thin-layer chromatography and near-infrared spectroscopy are the qualitative identification methods for TCM due to its features being fast and simple. 25 According to thin-layer chromatography spots in Fig. 1b, the spot blots were parallel to chromatographic profiles of reference substances based on standard of Tibetan medicines in Sichuan Province (2020).…”

Section: Resultsmentioning

confidence: 99%

Brassica rapa L. (Tibetan turnip) prevents sleep-deprivation induced cognitive deficits via the inhibition of neuroinflammation and mitochondrial depolarization

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Brassica rapa L. (Tibetan turnip) prevents sleep-deprivation induced cognitive deficits via the inhibition of neuroinflammation and mitochondrial depolarization

et al. 2022

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“…Therefore, appropriate sample processing procedures were required. Given the complexity of chemical composition in MTZSFG, systematic liquid‐liquid extraction with n ‐hexane, EtoAc, and n ‐BuOH was applied, which had been proved to be an effective method to deal with complex samples [53]. The water extract of MTZSFG was separated into three fractions of different polarities, which were analyzed separately.…”

Section: Discussionmentioning

confidence: 99%

Chemical profiling and mechanistic studies of Zhi‐Shang‐Feng granules against influenza virus by high‐performance liquid chromatography coupled with Q exactive focus hybrid quadrupole orbitrap high‐resolution mass spectrometry in combination with network pharmacology analysis

Xie,

Luo,

et al. 2023

J of Separation Science

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Zhi‐Shang‐Feng Granules are used in the clinical treatment of influenza to relieve headaches, chills and fever, bronchitis, nasal congestion, neuralgia and other symptoms. To decipher the components responsible for therapeutic effects of Zhi‐Shang‐Feng g ranules against influenza virus, an analytical method based on high‐performance liquid chromatography coupled with Q exactive focus hybrid quadrupole orbitrap high resolution mass spectrometry was developed and the chemical profile of Zhi‐Shang‐Feng granules was characterized. Then, the identified components were used to conduct network pharmacological analysis and determine the potential mechanism of Zhi‐Shang‐Feng Granules. As a result, 177 compounds were putatively identified through comprehensive analysis by liquid chromatography coupled with high‐resolution mass spectrometry, of which 23 compounds were unambiguously confirmed with reference standards. Components in Zhi‐Shang‐Feng Granules were found to specifically act on different enzymes, G‐protein‐coupled receptors, ion channels and transporters in the immune, endocrine, nervous, and circulatory systems. The potential mechanism was related to several biological processes, including cell growth and death, pattern recognition receptor signalling, signalling by interleukins, and lipid metabolism. The combination of chemical profile characterization and network construction provided useful insight into the overall chemical composition of Zhi‐Shang‐Feng granules and revealed their potential anti‐infection, anti‐inflammatory and immunoregulatory mechanisms against influenza virus infected disease.

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“…Manipulation of the pH and ionic strength of the aqueous phase facilitates the selective removal of ionizable compounds from the organic phase. Chen et al [67] used sequential extraction with solvents of different polarity (n-hexane, ethyl acetate, and n-butanol) for the systematic identification of multiple herbs in traditional Chinese medicines by comparison of the fingerprints obtained for each extract with those of individual reference standards. Nine out of sixteen herbs in a Daqinjiao decoction were identified in this way.…”

Section: Liquid-liquid Extractionmentioning

confidence: 99%

Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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High‐performance thin‐layer chromatography has favorable properties for high‐throughput separations with a high matrix tolerance. Sample preparation, however, is sometimes required to control specific matrix interferences and to enhance the detectability of target compounds. Trends in contemporary applications have shifted from absorbance and fluorescence detection to methods employing bioassays and mass spectrometry. Traditional methods (shake‐flask, heat at reflux, Soxhlet, and hydrodistillation) are being challenged by automated instrumental approaches (ultrasound‐assisted and microwave‐assisted solvent extraction, pressurized liquid extraction, and supercritical fluid extraction) and the quick, easy cheap, efficient, rugged, and safe extraction method for faster and streamlined sample processing. Liquid‐liquid extraction remains the most widely used approach for sample clean‐up with increasing competition from solid‐phase extraction. On‐layer sample, clean‐up by planar solid‐phase extraction is increasingly used for complex samples and in combination with heart‐cut multimodal systems. The automated spray‐on sample applicator, the elution head interface, biological detection of target and non‐target compounds, and straightforward mass spectrometric detection are highlighted as the main factors directing current interest toward faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants requiring high concentration factors.

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Exploration and practice on systematic identification strategy of traditional Chinese medicine prescriptions by high-performance thin-layer chromatography—with Daqinjiao decoction as an example

Cited by 5 publications

References 1 publication

Brassica rapa L. (Tibetan turnip) prevents sleep-deprivation induced cognitive deficits via the inhibition of neuroinflammation and mitochondrial depolarization

Brassica rapa L. (Tibetan turnip) prevents sleep-deprivation induced cognitive deficits via the inhibition of neuroinflammation and mitochondrial depolarization

Chemical profiling and mechanistic studies of Zhi‐Shang‐Feng granules against influenza virus by high‐performance liquid chromatography coupled with Q exactive focus hybrid quadrupole orbitrap high‐resolution mass spectrometry in combination with network pharmacology analysis

Sample preparation for planar chromatography

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