Exploration of rumen microbial and carbohydrate-active enzyme profiles in cattle fed coir a lignin-rich diet using a metagenomic approach

khatoon, Munni; Jakhesara, Subhash J.; Rank, D. N.; Joshi, Chaitanya; Kunjadiya, Anju

doi:10.1016/j.gene.2022.146868

Cited by 8 publications

(3 citation statements)

References 31 publications

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“…NEX enzymes displayed signi cant correlations with glycoside hydrolase genes at various time points, particularly within the Firmicutes, Actinomycetes, and some Bacteroidetes groups. Their heightened activity after the 24-hour mark underscores the signi cance of NEX enzymes in hemicellulose degradation [57].…”

Section: Discussionmentioning

confidence: 90%

Impact of Nutrient Composition on Rumen Microbiome Dynamics and Roughage Degradation

Ye,

Li,

et al. 2024

Preprint

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Background: Ruminant animals such as goats rely on rumen microbial communities to degrade fibrous nutrients from roughages, facilitating their growth and development. This study investigates dynamic shifts in surface-attached rumen microbes in representative roughages: rice straw (RS), bamboo shoot sheet (BSS), and alfalfa (ALF). Four 14-month-old Min Dong goats with rumen fistulas were used, and the roughages were assessed at 4 h, 12 h, 24 h, 36 h, 48 h, and 72 h intervals. Microbiome composition and function were revealed through 16S rRNA and metagenomics sequencing. Results: Prevotella and Treponema were the predominant genera in roughage degradation. Nutritional composition and tissue structure of roughages affected microbial attachment, causing variations in nutrient degradation rates. Microbials related to dry matter (DM) and crude protein (CP) degradation were abundant in early fermentation stages (4-12h) but decreased over time, while fiber-degrading microbials increased after 24 hours. Surface-attached microbials produced enzymes such as β-Glucosidase (BG), Endo-β-1,4-glucanase (C1), Exo-β-1,4-glucanase (Cx), and Neutral xylanase (NEX), with enzymatic activity correlating with the fiber content of the roughages. Conclusions: These findings advance our understanding of microbial roles in ruminant nutrition and digestion. The interaction between microbial communities and rumen fermentation is pivotal for understanding collaborative gene encoding by goat rumen microbiota, which is critical for fiber degradation.

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Section: Discussionmentioning

confidence: 90%

Impact of Nutrient Composition on Rumen Microbiome Dynamics and Roughage Degradation

Ye,

Li,

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…NEX enzymes displayed significant correlations with glycoside hydrolase genes at various time points, particularly within the Firmicutes, Actinomyceta, and some Bacteroidetes groups. Their heightened activity post the 24-hour mark underscores the significance of NEX enzymes in hemicellulose degradation [83].…”

Section: Discussionmentioning

confidence: 95%

Deciphering the Impact of Nutrient Composition and Tissue Structure on Rumen Microbiome Dynamics in Roughage Degradation

Ye,

Li,

et al. 2024

Preprint

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Background Ruminant animals, such as goats, depend on rumen microbial communities to degrade fibrous nutrients from roughages, facilitating their growth and development. This research delved into the dynamic shifts in surface-attached rumen microbes found in representative roughages (rice straw, bamboo shoot sheet, and alfalfa) and examined their degradation characteristics. Four 14-month-old Min Dong goats with rumen fistulas were used for the experiment, and the roughages were assessed at seven intervals (4 h, 12 h, 24 h, 36 h, 48 h, and 72 h). Using the 16S rRNA and metagenomics sequencing techniques to reveal the microbiome composition and their functions. Results Prevotella and Treponema were pinpointed as pivotal genera in roughage degradation. The nutritional composition and tissue structure of roughages affected microbial attachment, causing variations in nutrient degradation rates and the overall degradation process. Microbes related to dry matter (DM) and crude protein (CP) degradation were abundant in the early fermentation stages (4-12h) while decreased as time progressed. In contrast, microbes of fiber degradation increased gradually after 24-hour. Dominant in the goat rumen, Prevotella and Treponema are integral to roughage degradation, attributed to their multifaceted functional traits. Furthermore, the surface-attached microbes in the three roughages produced BG (β-Glucosidase), C1 (Endo-β-1,4-glucanase), Cx (Exo-β-1,4-glucanase), and NEX (Neutral xylanase) enzymes. The activity of these enzymes and their correlation with GHs (Glycoside Hydrolases) functional genes increased with the fiber content of the roughages. Conclusions These insights advance our understanding of microbial roles in ruminant nutrition and digestion. The interaction between microbial communities and rumen fermentation is pivotal to understanding the collaborative gene encoding by goat rumen microbiota being critical for fiber degradation.

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“…The use of rice husks in rations is very limited due to the presence of lignin compounds. The molecular structure of lignin complexes in lignocellulose material will prevent degradation by rumen microbial enzymes (Khatoon et al, 2022). These compounds are difficult to degrade by rumen microbes, so rice husks are difficult to digest by the digestive tract.…”

Section: Advances Inmentioning

confidence: 99%

The Relationship of Lignin and Crude Fiber in Rice Bran with Ultrasonic Wave Parameters

Rosani,

Hernaman,

Hidayat

et al. 2024

AAVS

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This study was designed to determine the ability of ultrasonic waves to detect rice husks added to rice bran based on physical properties associated with nutrient content. This research utilizes low-intensity ultrasonic waves of 50 kHz transmitted on rice bran mixed with rice husks with particle sizes passing 30 mesh (<0.595 mm). The purpose of this study was to determine the correlation between ultrasonic parameters (attenuation and velocity) and the nutrient content (crude fiber and lignin) of rice bran containing different rice husks. Based on the results of research, there is a strong relationship between ultrasonic parameters and the nutrient content of rice bran containing various rice husks. The positive correlation between attenuation with crude fibre is 0.975, and attenuation with lignin is 0.965. The attenuation coefficient can be used as a parameter for estimating the content of lignin and crude fibre in rice bran. This research proves the high nutritional value of rice bran can be detected with ultrasonic waves. These results still need further research to measure the factors that affect ultrasonic wave transmission (amplitude) when measuring samples, as well as the manufacture of a series of compact ultrasonic test systems so that they can be applied in industry or practically in the field.

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Exploration of rumen microbial and carbohydrate-active enzyme profiles in cattle fed coir a lignin-rich diet using a metagenomic approach

Cited by 8 publications

References 31 publications

Impact of Nutrient Composition on Rumen Microbiome Dynamics and Roughage Degradation

Impact of Nutrient Composition on Rumen Microbiome Dynamics and Roughage Degradation

Deciphering the Impact of Nutrient Composition and Tissue Structure on Rumen Microbiome Dynamics in Roughage Degradation

The Relationship of Lignin and Crude Fiber in Rice Bran with Ultrasonic Wave Parameters

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