Exploring an Innovative Strategy for Suppressing Bacterial Plant Disease: Excavated Novel Isopropanolamine-Tailored Pterostilbene Derivatives as Potential Antibiofilm Agents

Qi, Pu-Ying; Zhang, Taihong; Yang, Feng; Wang, Mingwei; Shao, Wu-Bin; Zhang, Dan; Jin, Linhong; Wang, Peiyi; Zhou, Xiang; Yang, Song

doi:10.1021/acs.jafc.2c00590

Cited by 31 publications

(51 citation statements)

References 45 publications

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Section: Resultssupporting

confidence: 91%

“…The higher the concentration of ethylicin, the more inhibition EPS production and biofilm formation. 30,52 Determination of the Extracellular Enzymes (Cellulase, Amylase, Protease). The virulence of Xanthomonas campestris pv.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…It is clear that ethylicin inhibited the production of the three extracellular enzymes of Xoc, a result that is consistent with previous studies and which follows the same trend as the results of the EPS production assay and biofilm formation. 50,52 Analysis of Differential Proteins. A total of 2754 proteins were identified using a false discovery rate (FDR) ≤0.01 as the filtering criterion.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…At concentrations of 4.24 and 2.12 μg/mL, the inhibition rate of EPS production and biofilm formation were significantly higher than that of 50 μg/mL SC (comparable at low concentrations of 1.10 μg/mL). The higher the concentration of ethylicin, the more inhibition EPS production and biofilm formation. , …”

Section: Resultsmentioning

confidence: 99%

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Ethylicin Inhibition of Xanthomonas oryzae pv. oryzicola In Vitro and In Vivo

Huang

Zhao

et al. 2023

J. Agric. Food Chem.

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Infestation of rice with the bacterium Xanthomonas oryzae pv. oryzicola (Xoc) causes the serious disease bacterial leaf streak (BLS). We studied the effect of ethylicin, a broad-spectrum bactericide, on Xoc both in vivo and in vitro. Ethylicin increases the defensive enzyme activities and defensive genes expression of rice. Ethylicin also significantly inhibited Xoc activity in vitro compared with other commercial bactericides. The half-maximal effective concentration (EC50) of ethylicin was 2.12 μg/mL. It has been shown that ethylicin can inhibit Xoc quorum sensing through the production of extracellular polysaccharides and enzymes, which disrupt the Xoc cell membrane. We used proteomic analysis to identify two oxidative phosphorylation pathway proteins (ACU12_RS13405 and ACU12_RS13355) which affected the virulence of Xoc and validated them using quantitative real-time polymerase chain reaction (qRT-PCR). The results indicate that ethylicin can increase the defense responses of rice and control Xoc proliferation.

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Section: Resultssupporting

confidence: 91%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Ethylicin Inhibition of Xanthomonas oryzae pv. oryzicola In Vitro and In Vivo

Huang

Zhao

et al. 2023

J. Agric. Food Chem.

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“…In Vitro Antibacterial Activity Evaluation [35] The in vitro antibacterial activities of 2a-2l and 3a-3f against XOO, XAC and PSA were determined at 100 and 50 μg/mL using a turbidimetric method. Experimental details and EC 50 calculation methods are included in the Supporting Information.…”

Section: General Synthetic Procedures For Target Compounds 3a-3fmentioning

confidence: 99%

Design, Synthesis, and Bioactivity of Eighteen Novel 2H‐1,4‐Benzoxazin‐3(4H)‐one Containing a Propanolamine Moiety

Rao

Zhou

Zhang

et al. 2022

Chemistry & Biodiversity

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To develop a novel skeleton for broad-spectrum pesticides with high-efficiency against tea tree diseases, a series of aniline 2H-1,4-benzoxazin-3(4H)-one derivatives containing a propanolamine structure was synthesized and confirmed by 1 H-NMR, 13 C-NMR, 19 F-NMR, HRMS, and single-crystal diffraction analysis. Bioactivities were evaluated against tobacco mosaic virus (TMV, the model virus), three kinds of bacteria, and five typical plant fungi. Bioassay results showed that compound 2i (EC 50 = 395.05 μg/mL) had the best curative activity against TMV, 3d (EC 50 = 45.70 μg/mL) had the best inhibitory activity against Pseudomonas syringae pv. Actinidiae, and 3a (EC 50 = 13.53 μg/mL) had the best inhibitory activity against Pestalotiopsis trachicarpicola. Scanning electron microscope morphological observation of P. trachicarpicola treated with 0, 100, and 200 μg/mL 3a revealed dried, flattened and folded outer walls of the hyphae at higher concentrations, leading to inhibition of fungal growth. The broad-spectrum bioactivities (against viruses, bacteria and fungi) of this series of target compounds indicate that these 2H-1,4-benzoxazin-3(4H)-one derivatives containing a propanolamine moiety are potential skeletons for developing pesticides with wide-ranging activities against various tea tree diseases.

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Photo‐Stimuli Smart Supramolecular Self‐Assembly of Azobenzene/β‐Cyclodextrin Inclusion Complex for Controlling Plant Bacterial Diseases

Yang

Xiang

et al. 2023

Adv Funct Materials

Self Cite

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Controllable and on‐demand delivery of supramolecular systems have received considerable attention in modern agricultural management, especially for managing intractable plant diseases. Here, an intelligent photoresponsive pesticide delivery system is reported based on β‐cyclodextrin (β‐CD) and azobenzene, which overcomes the resistance of phytopathogens caused by the irrational use of conventional pesticides. Antibacterial bioassays illustrated that designed azobenzene derivative 3a possesses the most efficient bioactivity with EC50 values of 0.52–25.31 µg mL−1 toward three typical phytopathogens. Moreover, the assembly of the supramolecular binary complex 3a@β‐CD is successfully elucidated and displays exceptional inhibitory activity on biofilm formation. Of note, this supramolecular binary complex significantly improves the water solubility, foliar surface wettability, and shows marked light‐responsive properties. In vivo anti‐Xoo assays reveal that 3a@β‐CD has excellent control efficiency (protective activity: 51.22%, curative activity: 48.37%) against rice bacterial blight pathogens, and their control efficiency can be elevated to values of 55.84% (protective activity) and 52.05% (curative activity) by UV–vis exposure. In addition, the 3a@β‐CD are non‐toxic toward various non‐target organisms. This study therefore offers new insights into the potential of host‐guest complexes as a feasible pesticide discovery strategy characterized by a safe, biocompatible, light‐responsive release, and antibiofilm properties for overcoming intractable plant bacterial diseases.

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Exploring an Innovative Strategy for Suppressing Bacterial Plant Disease: Excavated Novel Isopropanolamine-Tailored Pterostilbene Derivatives as Potential Antibiofilm Agents

Cited by 31 publications

References 45 publications

Ethylicin Inhibition of Xanthomonas oryzae pv. oryzicola In Vitro and In Vivo

Ethylicin Inhibition of Xanthomonas oryzae pv. oryzicola In Vitro and In Vivo

Design, Synthesis, and Bioactivity of Eighteen Novel 2H‐1,4‐Benzoxazin‐3(4H)‐one Containing a Propanolamine Moiety

Photo‐Stimuli Smart Supramolecular Self‐Assembly of Azobenzene/β‐Cyclodextrin Inclusion Complex for Controlling Plant Bacterial Diseases

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