Exploring indigenously produced celite-immobilized Rhizopus oryzae NRRL 3562-lipase for biodiesel production

Dash, Abhisek; Banerjee, Rintu

doi:10.1016/j.energy.2021.119950

Cited by 12 publications

(7 citation statements)

References 83 publications

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“…Moreover, the proposed process for obtaining the biocatalyst is much simpler. The efficiency of biodiesel synthesis using R. oryzae NRLL 3613 and R. oryzae DSM 63539 was similar to that obtained by other authors [19,30]. Our research has confirmed that the mesophilic strains of Rhizopus oryzae and Aspergillus oryzae are characterized by high biodiesel synthetic activity.…”

Section: Discussionsupporting

confidence: 90%

Mycelium-bound lipases of psychrophilic and mesophilic fungi as biocatalysts for biodiesel synthesis

Kutyła,

Jaszek,

Jędrys

et al. 2023

Preprint

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In this study, biocatalysts in the form of mycelium-bound lipases of psychrotrophic and mesophilic fungi were screened for their ability to synthesize fatty acid methyl (FAME) and ethyl esters (FAEE). It has been shown that the hydrolytic activity of biocatalysts is not accompanied by their biodiesel synthetic activity. Preliminary studies showed for the first time, that the FTIR technique can be used to predict synthetic activity. The highest correlation between the intensity of the bands and synthetic activity, negative and positive, respectively, was observed for two neighboring bands at 1745 cm-1(R= −0.73) and 1699 cm-1(R= 0.68). The psychrotrophic strainPenicilliumsp. 59, isolated from the soil of the Arctic tundra (Spitsbergen), has been shown for the first time as an efficient whole-cell biocatalyst for fatty acid ethyl ester synthesis and is stable in organic solvents. Mathematical optimization of biosynthesis was also performed using RSM. A molar canola oil conversion value of 83.3% into FAEE was obtained at 24.2 °C in n-hexane using 12.5% of used canola oil and 5.3% of the fungal biocatalyst within 6 h. Dry mycelium ofPenicilliumsp. 59 is a promising new biocatalyst for large-scale biosynthesis of biodiesel, given its low-cost production, high activity at room temperature, no need to mix reactions, and reusability in a minimum of six cycles.

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Section: Discussionsupporting

confidence: 90%

Mycelium-bound lipases of psychrophilic and mesophilic fungi as biocatalysts for biodiesel synthesis

Kutyła,

Jaszek,

Jędrys

et al. 2023

Preprint

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“…Multipoint imine bonds increase the stiffness of immobilized AOL particles and better resist various denaturing conditions (high temperature, pressure, or additives). Instead of physical forces, the formed covalent bonds between the AOL and MNC/PES fibers, instead of physical forces, thus prevent lipase leakage during reusability and leaching tests [2,4,10]. Our findings confirmed a report by Jacob et al [10] on the higher availability of free enzymatic active sites on the immobilized lipase, following the optimal use of enzyme loading during immobilization, concerning improvements in EV conversions.…”

Section: Regression Of Model Equation and Analysis Of Experimental Datasupporting

confidence: 88%

“…Electrospun nanofibrous materials exhibit high porosity and large specific surface area with controllable pore size. These materials have a very high flux, which makes them ideal for supporting enzymes [2][3][4]. Moreover, electrospun materials' customizable nature enables tuning their biocompatibility, i.e., nanoparticles [5][6][7], for different applications.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Magnetic Nanocellulose–Polyethersulfone-Conjugated Aspergillus oryzae Lipase for Synthesis of Ethyl Valerate

et al. 2021

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A novel greener MNC/PES membrane was developed through an electrospinning technique for lipase immobilization to catalyze the synthesis of ethyl valerate (EV). In this study, the covalent immobilization of Aspergillus oryzae lipase (AOL) onto an electrospun nanofibrous membrane consisting of magnetic nanocellulose (MNC) and polyethersulfone (PES) to produce EV was statistically optimized. Raman spectroscopy, Fourier-transform infrared spectroscopy: attenuated total reflection, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, thermal gravimetric analysis (TGA), and differential thermal gravimetric (DTG) of MNC/PES-AOL demonstrated that AOL was successfully immobilized onto the fibers. The Taguchi design-assisted immobilization of AOL onto MNC/PES fibers identified that 1.10 mg/mL protein loading, 4 mL reaction volume, 250 rpm stirring rate, and 50 °C were optimal to yield 72.09% of EV in 24 h. The thermal stability of MNC/PES-AOL was improved by ≈20% over the free AOL, with reusability for up to five consecutive esterification cycles while demonstrating an exceptional half-life of 120 h. Briefly, the electrospun MNC/PES fibers that immobilized AOL showed promising applicability in yielding relatively good EV levels. This study suggests that using MNC as fillers in a PES to improve AOL activity and durability for a longer catalytic process could be a viable option.

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“…The effect of the cultivation conditions on the fungal biomass transesterification of vegetable oils has been studied by several members of Mucorales [ 20 , 21 ]. Physical factors such as fermentation temperature, initial pH, and agitation rate were critical for producing whole-cell lipase catalysts.…”

Section: Introductionmentioning

confidence: 99%

Rhizopus stolonifer biomass catalytic transesterification capability: optimization of cultivation conditions

et al. 2023

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Background Using fungal biomass for biocatalysis is a potential solution for the expensive cost of the use o enzymes. Production of fungal biomass with effective activity requires optimizing the cultivation conditions. Results Rhizopus stolonifer biomass was optimized for transesterification and hydrolysis of waste frying oil (WFO). Growth and biomass lipolytic activities of R. stolonifer improved under shaking conditions compared to static conditions, and 200 rpm was optimum. As biomass lipase and transesterification activities inducer, olive oil was superior to soybean, rapeseed, and waste frying oils. Biomass produced in culture media containing fishmeal as an N-source feedstock had higher lipolytic capabilities than corn-steep liquor and urea. Plackett Burman screening of 9 factors showed that pH (5–9), fishmeal (0.25–1.7%, w/v), and KH2PO4 (0.1–0.9%, w/v) were significant factors with the highest main effect estimates 11.46, 10.42, 14.90, respectively. These factors were selected for response surface methodology (RSM) optimization using central composite design (CCD). CCD models for growth, biomass lipase activity, and transesterification capability were significant. The optimum conditions for growth and lipid modification catalytic activities were pH 7.4, fishmeal (2.62%, w/v), and KH2PO4 (2.99%, w/v). Conclusion Optimized culture conditions improved the whole cell transesterification capability of Rhizopus stolonifer biomass in terms of fatty acid methyl ester (FAME) concentration by 67.65% to a final FAME concentration of 85.5%, w/w.

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Exploring indigenously produced celite-immobilized Rhizopus oryzae NRRL 3562-lipase for biodiesel production

Cited by 12 publications

References 83 publications

Mycelium-bound lipases of psychrophilic and mesophilic fungi as biocatalysts for biodiesel synthesis

Mycelium-bound lipases of psychrophilic and mesophilic fungi as biocatalysts for biodiesel synthesis

Electrospun Magnetic Nanocellulose–Polyethersulfone-Conjugated Aspergillus oryzae Lipase for Synthesis of Ethyl Valerate

Rhizopus stolonifer biomass catalytic transesterification capability: optimization of cultivation conditions

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