Exploring Novel Isocractic HPLC Method for Quantitative Determination of Cinnarizine and Piracetam in Their Capsule Preparations

Sm, El-Adl; Me, El-Sadek; Mh, Hasan

doi:10.21065/1920-4159.1000225

J App Pharm

2016

DOI: 10.21065/1920-4159.1000225

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Exploring Novel Isocractic HPLC Method for Quantitative Determination of Cinnarizine and Piracetam in Their Capsule Preparations

El-Adl Sm¹,

El-Sadek Me²,

Hasan Mh³

Abstract: A novel isocratic HPLC method had been developed for rapid simultaneous separation and determination of cinnarizine and Piracetam in pure form or in pharmaceutical formulations within less than 10 minutes. Separation was carried out on a Hypersil gold C 18 (10µm, 100x4.6mm) column. Effect of pH and composition of mobile phase in addition to flow rate was studied. Calibration was obeyed in the range of (10-80) µg/ml for cinnarizine or (160-960) µg/ ml for Piracetam. The method was applied for the simultaneous d… Show more

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Simple Eco-Friendly RP-LC Method for the Synchronous Separation of Six Widely Used Drugs in the Treatment of Cerebrovascular and Vestibular Disorders: Application for Analysis in Their Single and Combined Drug Products

El-Houssini

Mohammad

2022

Journal of AOAC INTERNATIONAL

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Background RP-LC is considered the most extensively used chromatographic technique in drug separation and quantification; yet its routine use shows increasing environmental challenges about energy consumption, solvent used and waste engendered. From this perception, efforts for Greening the developed RP-LC methods are focused on using of green solvents; reducing the amount of solvent used and waste produced. Objective The target of this work is to develop eco-friendly RP-LC method for the synchronous separation of six widely used drugs in the treatment of cerebrovascular and vestibular disorders namely; neurotropic Piracetam, antihistamines, Dimenhydrinate, Cinnarizine and Fluranizine, vasodilators Vincamine and Vinpocetine. Method Separation and quantification are accomplished at ambient temperature by isocratic elution mode using acetonitrile–0.2% triethylamine (85:15; by volume) as a mobile phase at a flow rate of 1.5 mL/min on Hypersil C18 column. UV detection and quantification are carried out at 225 nm. Results The method is fully validated per ICH guidelines, where all analytical parameters are within acceptable range, r > 0.9999, accuracy ≥99.55% and precision (0.02–0.97%). Conclusion The method is applied for the routine analysis of the cited drugs in their single and combined drug products and it is considered excellent in terms of greenness with respect to Eco-Scale assessment. Highlights As the routine use of RP-LC technique shows increasing environmental challenges, efforts for Greening are attempted focusing on the use of green solvents; reduce the amount of solvent used and waste produced. A simple eco-friendly RP-LC method for the synchronous separation of six widely used drugs in the treatment of cerebrovascular and vestibular disorders is developed. The method is fully validated per ICH guidelines, where all analytical parameter are within acceptable range. -The method is applied for the routine analysis of the cited drugs in their single and combined drug products.

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Simple Eco-Friendly RP-LC Method for the Synchronous Separation of Six Widely Used Drugs in the Treatment of Cerebrovascular and Vestibular Disorders: Application for Analysis in Their Single and Combined Drug Products

El-Houssini

Mohammad

2022

Journal of AOAC INTERNATIONAL

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Use Of Chemometrics For Development And Validation Of RP-HPLC Method For Simultaneous Determination Of Dimenhydrinate And Cinnarizine

Jayaseelan¹,

Kannappan

Ganesan³

2022

Int J Life Sci Pharm Res

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A novel simple, sensitive, rapid and accurate isocratic RP-HPLC method was developed and validated for simultaneous determination of Dimenhydrinate and Cinnarizine applying statistical experimental design. Design of experiments (DoE) was applied for multivariate optimization of the experimental conditions of the RP-HPLC method. Three independent factors like; mobile phase composition, phosphate buffer strength and flow rate were employed to design mathematical models. Central composite design (CCD) was applied to study the response surface methodology. This study was used to find the deepness effects of these independent factors. Desirability function was used to simultaneously optimize the retention time and resolution of the analytes. Hence, our present study was aimed in finding the optimized and predicted data from the contour diagram. Mobile phase consisted of acetonitrile and ammonium acetate buffer (pH 7.0, strength 0.3 mM) in the ratio of 55:45%v/v respectively, at a flow rate of 1.7 mL/min was used. The present study objectives were to apply these optimum conditions baseline partition of both drugs with good resolution. The run time of less than 12.0 min was accomplished. The optimized assay conditions were validated according to ICH guidelines. The correlation coefficient value was found to be r2 = 0.9990 and 0.9993 for Dimenhydrinate and Cinnarizine respectively. LOD and LOQ value was found to be least concentration for both drugs. It indicates the method was highly sensitive. The accuracy %RSD value was found to be less than 2%. Consequently the reports clearly showed that Quality by design approach could be triumphantly applied to optimize RP-HPLC method for simultaneous determination of Dimenhydrinate and Cinnarizine.

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Exploring Novel Isocractic HPLC Method for Quantitative Determination of Cinnarizine and Piracetam in Their Capsule Preparations

Cited by 2 publications

References 15 publications

Simple Eco-Friendly RP-LC Method for the Synchronous Separation of Six Widely Used Drugs in the Treatment of Cerebrovascular and Vestibular Disorders: Application for Analysis in Their Single and Combined Drug Products

Simple Eco-Friendly RP-LC Method for the Synchronous Separation of Six Widely Used Drugs in the Treatment of Cerebrovascular and Vestibular Disorders: Application for Analysis in Their Single and Combined Drug Products

Use Of Chemometrics For Development And Validation Of RP-HPLC Method For Simultaneous Determination Of Dimenhydrinate And Cinnarizine

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