Exploring photodynamic inactivation to combat antimicrobial resistance

Abstract

The rise of antimicrobial-resistant microorganisms prompts the exploration of strategies to rejuvenate traditional antibiotics. Photodynamic Inactivation (PDI) offers an alternative by combining a photosensitizer (PS) with light, generating reactive oxygen species that efficiently eliminate undesirable cells. In this study, curcumin and 450 nm light are applied, resulting in reduced minimum inhibitory concentrations (MIC) for antibiotics. The application of PDI proves effective in diminishing the necessary antibiotic dosage for bacterial cell elimination, especially against resistant strains. Additionally, the interaction between the photosensitizer and antibiotics induces notable alterations in bacterial metabolism and biomolecules. These findings are crucial for advancing from in vitro studies to the clinical implementation of PDI as a promising adjunct to antibiotic therapy, offering potential solutions in the battle against antimicrobial resistance.